SAXS data of Legionella pneumophila phosphocholine hydrolase Lem3(19-570)

{
  "code": "SASDCN8",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "The structural analysis of dephosphocholinase Legionella pneumophila Lem3",
    "publication": null,
    "status": "released",
    "submitted_date": "2017-10-25",
    "released_date": "2024-10-25"
  },
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "CCD",
        "name": "AVIEX PCCD170170",
        "resolution": null
      },
      "name": "SOLEIL",
      "city": "Saint-Aubin",
      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Phosphocholine hydrolase Lem3",
          "short_name": "Lem3",
          "sequence": "MRDKIITGKKIIFSQSVAKDQTKNLSSFLSERFYSVNQSHNHSIIIGSSLSH\r\nQENDIEHDTILDTSGVLVTTDTNGIVNGARVAITDGLGGGNGDQEEDDEIYRVSHSSCENFLNCDQNIDT\r\nTLSLITQPKASDKKQTAPKTLQHTEASMAAFIYQNHPGKGYIGEFANIGDGLIIILDKRFKIKHMVSACH\r\nIYRGFGTWTPPSLQALATTANKDALLVRQTLKLAEGDIIISMTDGVWGELKTSLIAQTNDRRDIGVDKEY\r\nFKTLFDELTDAPYPSSFDIARIITQRAMSRSLERRKTLIKLINEIEQQHFHEKSVKTINEVLEYFIKTGH\r\nVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTVGDCSTINVTRIPYHLDELIRGFINYPEK\r\nHQILAPLFKARVKSEADLEEAFHRLSLEMVQPEIECPISETHFERAFKKETLDKTQAVLTHYFRISTGLD\r\nSKKNYQERLNDLSAYLSKESSLEKNDIKLLLSMLDSEIKPKTGVFQTLFGENQNKLYKAFHKKIELQLLD\r\nSEIENKNELKHHHHHH",
          "organism": "Legionella pneumophila subsp. pneumophila",
          "uniprot_code": "Q5ZXN5",
          "uniprot_range_first": 19,
          "uniprot_range_last": 570,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MKLRYIINENKLVFTSCNMRDKIITGKKIIFSQSVAKDQTKNLSSFLSERFYSVNQSHNH\nSIIIGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNGARVAITDGLGGGNGDQEEDDEI\nYRVSHSSCENFLNCDQNIDTTLSLITQPKASDKKQTAPKTLQHTEASMAAFIYQNHPGKG\nYIGEFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTWTPPSLQALATTANKDALLVRQT\nLKLAEGDIIISMTDGVWGELKTSLIAQTNDRRDIGVDKEYFKTLFDELTDAPYPSSFDIA\nRIITQRAMSRSLERRKTLIKLINEIEQQHFHEKSVKTINEVLEYFIKTGHVETAQTLKAI\nLFEDGLSDGITYFENIEIPLEMVMHDLKSRTVGDCSTINVTRIPYHLDELIRGFINYPEK\nHQILAPLFKARVKSEADLEEAFHRLSLEMVQPEIECPISETHFERAFKKETLDKTQAVLT\nHYFRISTGLDSKKNYQERLNDLSAYLSKESSLEKNDIKLLLSMLDSEIKPKTGVFQTLFG\nENQNKLYKAFHKKIELQLLDSEIENKNELK",
          "mw": 63.391,
          "total_mw": 63.391,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "SAXS data of Legionella pneumophila phosphocholine hydrolase Lem3(19-570)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Cachan, France",
            "full_name": "CNRS UMR8113, ENS Paris-Saclay",
            "webpage": "http://lbpa.ens-paris-saclay.fr/"
          }
        ],
        "contributor_name": "Wenhua",
        "contributor_surname": "Zhang",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-09-20",
    "storage_temperature": null,
    "cell_temperature": 15.0,
    "exposure_time": 1.5,
    "number_of_frames": 1,
    "wavelength": 0.102,
    "sample_detector_distance": 1.2,
    "concentration_min": 4.0,
    "concentration_max": 6.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.062,
    "s_max": 5.294,
    "total_exposure_time": null,
    "seccolumn": null
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  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "2.8.2",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of SAXS data of Legionella pneumophila phosphocholine hydrolase (19-570) in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.102 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle).  Solute concentrations ranging between 4 and 6 mg/ml were measured at 15°C for a total exposure time of 1.5 s. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled for protein concentration.",
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 66.7,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 67.0,
  "porod_mw_error": null,
  "pddf_i0": 0.05143,
  "pddf_i0_error": 2e-05,
  "guinier_i0": 0.05142,
  "guinier_i0_error": 6.1e-05,
  "pddf_rg": 3.52,
  "pddf_rg_error": 0.002,
  "guinier_rg": 3.46,
  "guinier_rg_error": 0.08,
  "pddf_dmax": 12.2,
  "pddf_dmax_error": null,
  "porod_volume": 95.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 19,
  "guinier_point_last": 46,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCN8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2024-10-31T15:53:27.134585+01:00",
  "bragg_peak": []
}

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