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SASDCN9

Ks-Amt5-HK (apo)

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Ks-Amt5-HK (apo)

Concentration1.0 – 5.0 Buffer / pH20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol / 8.0
Experimental temperature20.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Ammonium transporter histidine kinase domain
View sequence
FIRITRLQDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSAELRTPLNAIIGFAEVLRDEIAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTIINASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNSLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAESTFGKGSTFFFILPINPVNK
proteinCandidatus Kuenenia stuttgartiensisQ1Q357—–—monomerMolecule count 130.031 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11613[3]0.144989–4.42296 1/nmHas uncertainty columnMissing 014sasbdb/entries/n9/sasdcn9/source/SASDCN9.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.00.5nm
i0Guinier2046.09.0arbitrary
i0P(r)2065.07.0arbitrary
mwExperimental32.6—kDa
mwGuinier I(0)32.63.0kDa
mwPorod42.0—kDa
porod_volumePorod71.0—nm³
rgGuinier2.70.1nm
rgP(r)2.81.0nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerolBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-03-02Storage / measurement temperature20.0 / 20.0
Exposure time0.05Frames20
Wavelength0.124Sample-to-detector distance2.7
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.145 – 4.423Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zip：source record and ZIP contents (2 items)
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pddf：source record
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sascif：source record
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summary：source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：134 field values
Field pathRaw value
codeSASDCN9
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleSignaling ammonium across membranes through an ammonium sensor histidine kinase.
project.publication.titleSignaling ammonium across membranes through an ammonium sensor histidine kinase.
project.publication.author_listPflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-017-02637-3
project.publication.pmid29323112
project.publication.published_date2018 Jan 11
project.statusreleased
project.submitted_date2017-11-29
project.released_date2018-01-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDCN9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDCN9.dat
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCN9_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCN9_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCN9_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDCN9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAmmonium transporter histidine kinase domain
experiment.sample.molecule[0].short_nameKs-Amt5-HK
experiment.sample.molecule[0].sequenceFIRITRLQDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSAELRTPLNAIIGFAEVLRDEIAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTIINASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNSLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAESTFGKGSTFFFILPINPVNK
experiment.sample.molecule[0].organismCandidatus Kuenenia stuttgartiensis
experiment.sample.molecule[0].uniprot_codeQ1Q357
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMENIQININHLWVIMAACMVFLMQLGFTSYETGFSQSKNAISIALRNLVDTLISSLVFFS VGFGFMFGKSYMGLIGIDLFFANDLALHPNTLSYSFFFFQMVFASTAATILTGAIAERSG FIPNIAGTAFIVAIIYPIFGHWAWGNLFSPDQTGWLKELGFIDFAGATVVHSIGGWFAMA AAIMVGPRIDKYNPDGSSNRIGLHNVPLATLGTFFLWFGWFGFNGGSLLRVSVNIGLVIL NTNMAAASAGVSALIFIYATRKRIEAGSLFTAILAGLVAITASSNMVTPVSAVAIGLITG ILAIIAEGFIEKTLKIDDPVSAIAVHGVGGVIGTLCVAIFAQKSYLLAENGSRMHQLGIQ ALGVIVAFSWSFGLGMLFFLCLKKVKRLRVTPEEEKRGLNVAEYEDVASWLDFIRITRLQ DINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSHELRTPLNAIIGFAEVLRDEI AGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTII NASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNS LQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAES TFGKGSTFFFILPINPVNK
experiment.sample.molecule[0].mw30.031
experiment.sample.molecule[0].total_mw30.031
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameKs-Amt5-HK (apo)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-03-02
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance2.7
experiment.concentration_min1.0
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.145
experiment.s_max4.423
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw32.6
experimental_mw_errornull
guinier_i0_mw32.6
guinier_i0_mw_error3.0
porod_mw42.0
porod_mw_errornull
pddf_i02065.0
pddf_i0_error7.0
guinier_i02046.0
guinier_i0_error9.0
pddf_rg2.8
pddf_rg_error1.0
guinier_rg2.7
guinier_rg_error0.1
pddf_dmax10.0
pddf_dmax_error0.5
porod_volume71.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last127
pddf_point_first2
pddf_point_last1200
i0_calibration_standard_datanull
intensities_log_log_plotSASDCN9_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json：36 field values
Field pathRaw value
codeSASDCN9
statussuccess
started_at2026-08-11T16:18:16.841786+00:00
finished_at2026-08-11T16:18:28.583685+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDCN9",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Signaling ammonium across membranes through an ammonium sensor histidine kinase.",
    "publication": {
      "title": "Signaling ammonium across membranes through an ammonium sensor histidine kinase.",
      "author_list": "Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-017-02637-3",
      "pmid": "29323112",
      "published_date": "2018 Jan 11"
    },
    "status": "released",
    "submitted_date": "2017-11-29",
    "released_date": "2018-01-16"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCN9.out",
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  "experiment": {
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        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Ammonium transporter histidine kinase domain",
          "short_name": "Ks-Amt5-HK",
          "sequence": "FIRITRLQDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSAELRTPLNAIIGFAEVLRDEIAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTIINASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNSLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAESTFGKGSTFFFILPINPVNK",
          "organism": "Candidatus Kuenenia stuttgartiensis",
          "uniprot_code": "Q1Q357",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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          "mw": 30.031,
          "total_mw": 30.031,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ks-Amt5-HK (apo)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
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            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
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        "contributor_name": "Haydyn",
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        "orcid": null
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    "concentration_unit": null,
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    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.05,
    "number_of_frames": 20,
    "wavelength": 0.124,
    "sample_detector_distance": 2.7,
    "concentration_min": 1.0,
    "concentration_max": 5.0,
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    "s_min": 0.145,
    "s_max": 4.423,
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    "seccolumn": null
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  "tags": [],
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View the complete manifest.json source
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