SASDCP5

Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Other 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)

浓度— – — 缓冲液 / pH25 mM Tris-HCl, 300 mM NaCl / 7.5
实验温度20.0 设备 / 束线ESRF / BM29
波长0.0931 nm曝光1.0 s × 3000

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
DNA polymerase processivity factor component A20 C-ter fragment
查看序列
GNGKYFSKVGSAGLKQLTNKLDINECATVDELVDEINKSGTVKRKIKNQSAFDLSRECLGYPEADFITLVNNMRFKIENCKVVNFNIENTNCLNNPSIETIYRNFNQFVSIFNVVTDVKKRLFENASGNGSGGGLNDIFEAQKIEWHE
proteinVaccinia virusP20995304–426monomer分子数 116.65 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.03286978–4.944755 1/nm含误差列缺失 026sasbdb/entries/p5/sasdcp5/source/SASDCP5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.4—nm
i0Guinier5.590.01—
i0P(r)5.580.01—
mwExperimental19.0—kDa
mwPorod19.0—kDa
porod_volumePorod31.0—nm³
rgGuinier2.150.01nm
rgP(r)2.150.01nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Tris-HCl, 300 mM NaCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-02-05储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数3000
波长0.0931样品-探测器距离2.34
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.033 – 4.945样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/p5/sasdcp5/source/SASDCP5.zipdownloaded553903f9f45fe2a2d22266f4dbc3b6dbfd3d9f2e2bbd8bb1c6a354c5ff20c5d731485下载查看原文件源站
pddfsasbdb/entries/p5/sasdcp5/source/SASDCP5.outdownloaded612033e97355438d91eb979b7c1a4aef4194e8e289fa6c9f0bebd4a43ae722434fa09下载查看原文件源站
sascifsasbdb/entries/p5/sasdcp5/source/SASDCP5.sascifdownloaded3137092a91ed024193433080b1767f65ad8793f89c58d39b41f553be367c4c549153ed下载查看原文件源站
summarysasbdb/entries/p5/sasdcp5/source/summary.jsondownloaded77094f57715441bc0d1c72c04e5d88fdac9bc636977da3aa8523fbac532e93d7dd9e下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDCP5
statusPublished
type_of_curveOther
angular_unit1/nm
project.titleThe vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.
project.publication.titleThe vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.
project.publication.author_listTarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-017-01542-z
project.publication.pmid29129932
project.publication.published_date2017 Nov 13
project.statusreleased
project.submitted_date2017-06-27
project.released_date2017-11-20
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experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
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experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDNA polymerase processivity factor component A20 C-ter fragment
experiment.sample.molecule[0].short_nameA20 C-ter
experiment.sample.molecule[0].sequenceGNGKYFSKVGSAGLKQLTNKLDINECATVDELVDEINKSGTVKRKIKNQSAFDLSRECLGYPEADFITLVNNMRFKIENC KVVNFNIENTNCLNNPSIETIYRNFNQFVSIFNVVTDVKKRLFENASGNGSGGGLNDIFEAQKIEWHE
experiment.sample.molecule[0].organismVaccinia virus
experiment.sample.molecule[0].uniprot_codeP20995
experiment.sample.molecule[0].uniprot_range_first304
experiment.sample.molecule[0].uniprot_range_last426
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw16.65
experiment.sample.molecule[0].total_mw16.65
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM Tris-HCl, 300 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameVaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameIBS
experiment.contributor[0].affiliation[0].addressGrenoble, France
experiment.contributor[0].affiliation[0].full_nameInstitut de Biologie Structurale
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameWim
experiment.contributor[0].contributor_surnameBurmeister
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-02-05
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames3000
experiment.wavelength0.0931
experiment.sample_detector_distance2.34
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.033
experiment.s_max4.945
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_version2.7
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fits[0].models[0].model_mw21.8
fits[0].models[0].bead_radius1.6
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fits[0].models[0].symmetrynone
fits[0].models[0].commentbest model of 40
fits[0].models[0].user226
fits[0].fit_unit1/A
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fits[0].chi_square_value0.254
fits[0].p_value0.000148
fits[0].fit_residual_plotSASDCP5_fit1_fitresiduals_img.png
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fits[0].software_version
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of A20 C-ter fragment in 25 mM Tris-HCl, 300 mM NaCl, pH 7.5 were collected using size exclusion chromatography coupled SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.3 m and at a wavelength of λ = 0.0931 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were collected at at 20°C during the course of elution (3000 successive 1 second frames). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the resulting subtracted curves were scaled and averaged.
tags[]
intensity_unitnull
experimental_mw19.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw19.0
porod_mw_errornull
pddf_i05.58
pddf_i0_error0.01
guinier_i05.59
guinier_i0_error0.01
pddf_rg2.15
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guinier_rg2.15
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pddf_dmax_errornull
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porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first18
guinier_point_last88
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCP5_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCP5
statussuccess
started_at2026-08-11T14:52:24.616676+00:00
finished_at2026-08-11T14:52:32.969307+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDCP5",
  "status": "Published",
  "type_of_curve": "Other",
  "angular_unit": "1/nm",
  "project": {
    "title": "The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.",
    "publication": {
      "title": "The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.",
      "author_list": "Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-017-01542-z",
      "pmid": "29129932",
      "published_date": "2017 Nov 13"
    },
    "status": "released",
    "submitted_date": "2017-06-27",
    "released_date": "2017-11-20"
  },
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      "country": "France",
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    },
    "sample": {
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        {
          "long_name": "DNA polymerase processivity factor component A20 C-ter fragment",
          "short_name": "A20 C-ter",
          "sequence": "GNGKYFSKVGSAGLKQLTNKLDINECATVDELVDEINKSGTVKRKIKNQSAFDLSRECLGYPEADFITLVNNMRFKIENC\r\nKVVNFNIENTNCLNNPSIETIYRNFNQFVSIFNVVTDVKKRLFENASGNGSGGGLNDIFEAQKIEWHE",
          "organism": "Vaccinia virus",
          "uniprot_code": "P20995",
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          "total_mw": 16.65,
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
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}
查看完整 manifest.json 原文
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