SASDCP8

Ras-related protein Rab-1A

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2024-10-31T15:53:47.550615+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Ras-related protein Rab-1A

浓度2.0 – 6.0 缓冲液 / pH300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl / 7.5
实验温度15.0 设备 / 束线SOLEIL / SWING
波长0.103 nm曝光1.2 s × 240

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Ras-related protein Rab-1A
查看序列
MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCCHHHHHH
proteinHomo sapiensP62820—–—monomer分子数 123.5 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11062[3]0.01690595–0.6124736 1/A含误差列缺失 013sasbdb/entries/p8/sasdcp8/source/SASDCP8.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.2—Å
i0Guinier0.013661.3e-051/cm
i0P(r)0.013851.4e-051/cm
mwExperimental24.0—kDa
mwPorod24.0—kDa
porod_volumePorod36.1—ų
rgGuinier2.380.004Å
rgP(r)2.540.006Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-11-17储存 / 测量温度— / 15.0
曝光时间1.2帧数240
波长0.103样品-探测器距离1.2
光源X-ray synchrotron探测器AVIEX PCCD170170
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q 范围0.169 – 6.125样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/p8/sasdcp8/source/SASDCP8.zipdownloaded5329885a61c8f73153161e1b068ab525a1ac1e2ac6c9a7c0e10080b7939eec3b0c4e7下载查看原文件源站
pddfsasbdb/entries/p8/sasdcp8/source/SASDCP8.outdownloaded3803908b4f132360510ef2ec9fbdf56a9a1f38f9946b9b7a8607ca7af31b1c6aaa5f3下载查看原文件源站
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summarysasbdb/entries/p8/sasdcp8/source/summary.jsondownloaded708137da1e30e51751769e20221df5814ea39b0695a23f1fcfbfd687ac7edf0a9e64下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDCP8
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleThe SAXS Analysis of the human GTPase Rab1a
project.publicationnull
project.statusreleased
project.submitted_date2017-10-29
project.released_date2024-10-29
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experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameRas-related protein Rab-1A
experiment.sample.molecule[0].short_nameRab1a
experiment.sample.molecule[0].sequenceMSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQ ERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAK EFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCCHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP62820
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTI KLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKL LVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGA TAGGAEKSNVKIQSTPVKQSGGGCC
experiment.sample.molecule[0].mw23.5
experiment.sample.molecule[0].total_mw23.5
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameRas-related protein Rab-1A
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressCachan, France
experiment.contributor[0].affiliation[0].full_nameCNRS UMR8113, ENS Paris-Saclay
experiment.contributor[0].affiliation[0].webpagehttp://lbpa.ens-paris-saclay.fr/
experiment.contributor[0].contributor_nameWenhua
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-17
experiment.storage_temperaturenull
experiment.cell_temperature15.0
experiment.exposure_time1.2
experiment.number_of_frames240
experiment.wavelength0.103
experiment.sample_detector_distance1.2
experiment.concentration_min2.0
experiment.concentration_max6.0
experiment.sample_volumenull
experiment.flow_ratenull
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experiment.s_max6.125
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].model_mw24.2
fits[0].models[0].bead_radius1.4
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fits[0].models[0].symmetryP1
fits[0].models[0].comment
fits[0].models[0].user84
fits[0].fit_unit1/A
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fits[0].softwareDAMMIF
fits[0].chi_square_value1.745
fits[0].p_value5e-06
fits[0].fit_residual_plotSASDCP8_fit1_fitresiduals_img.png
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fits[0].software_version2.8.2
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version2.8.2
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Ras-related protein Rab-1A in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5, were collected at the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 2 and 6 mg/ml were measured at 15°C. 240 successive 1.2second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.
tags[]
intensity_unit1/cm
experimental_mw24.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw24.0
porod_mw_errornull
pddf_i00.01385
pddf_i0_error1.4e-05
guinier_i00.01366
guinier_i0_error1.3e-05
pddf_rg2.54
pddf_rg_error0.006
guinier_rg2.38
guinier_rg_error0.004
pddf_dmax10.2
pddf_dmax_errornull
porod_volume36.1
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first11
guinier_point_last59
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCP8_datloglog_img.png
symmetrynull
last_modified2024-10-31T15:53:47.550615+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCP8
statussuccess
started_at2026-08-11T15:57:42.652391+00:00
finished_at2026-08-11T15:57:51.249364+00:00
source_last_modified2024-10-31T15:53:47.550615+01:00
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查看完整 summary.json 原文
{
  "code": "SASDCP8",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "The SAXS Analysis of the human GTPase Rab1a",
    "publication": null,
    "status": "released",
    "submitted_date": "2017-10-29",
    "released_date": "2024-10-29"
  },
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          "molecular_type": "protein",
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      },
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}
查看完整 manifest.json 原文
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