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SASDCQ9

Ks-Amt5-HK (+10uM ATP)

数据类型：SASBDB 实验数据 状态：Published 曲线类型：Merged 最后更新：2023-06-21T07:56:39.492710+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Ks-Amt5-HK (+10uM ATP)

浓度1.0 – 5.0 缓冲液 / pH20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol / 8.0
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Ammonium transporter histidine kinase domain
查看序列
FIRITRLQDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSAELRTPLNAIIGFAEVLRDEIAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTIINASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNSLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAESTFGKGSTFFFILPINPVNK
proteinCandidatus Kuenenia stuttgartiensisQ1Q357—–—monomer分子数 130.031 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11604[3]0.168858–4.42296 1/nm含误差列缺失 013sasbdb/entries/q9/sasdcq9/source/SASDCQ9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.70.5nm
i0Guinier1795.07.0arbitrary
i0P(r)1801.06.0arbitrary
mwExperimental28.6—kDa
mwGuinier I(0)28.63.0kDa
mwPorod31.0—kDa
porod_volumePorod53.0—nm³
rgGuinier2.20.1nm
rgP(r)2.20.1nm

这些数值是 SASBDB 来源记录，不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”；未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-03-02储存 / 测量温度20.0 / 20.0
曝光时间0.05帧数20
波长0.124样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.169 – 4.423样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve：来源记录
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full_entry_zip：来源记录与 ZIP 内部目录（2 项）
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pddf：来源记录
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sascif：来源记录
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summary：来源记录
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全部来源字段（无筛选）

这里自动展开来源记录中的每一个字段，包括空值、列表成员和页面上方已展示过的字段。

summary.json：134 个字段值
字段路径原始值
codeSASDCQ9
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleSignaling ammonium across membranes through an ammonium sensor histidine kinase.
project.publication.titleSignaling ammonium across membranes through an ammonium sensor histidine kinase.
project.publication.author_listPflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-017-02637-3
project.publication.pmid29323112
project.publication.published_date2018 Jan 11
project.statusreleased
project.submitted_date2017-11-29
project.released_date2018-01-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDCQ9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDCQ9.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ9_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ9_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCQ9_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ9_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDCQ9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAmmonium transporter histidine kinase domain
experiment.sample.molecule[0].short_nameKs-Amt5-HK
experiment.sample.molecule[0].sequenceFIRITRLQDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSAELRTPLNAIIGFAEVLRDEIAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTIINASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNSLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAESTFGKGSTFFFILPINPVNK
experiment.sample.molecule[0].organismCandidatus Kuenenia stuttgartiensis
experiment.sample.molecule[0].uniprot_codeQ1Q357
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMENIQININHLWVIMAACMVFLMQLGFTSYETGFSQSKNAISIALRNLVDTLISSLVFFS VGFGFMFGKSYMGLIGIDLFFANDLALHPNTLSYSFFFFQMVFASTAATILTGAIAERSG FIPNIAGTAFIVAIIYPIFGHWAWGNLFSPDQTGWLKELGFIDFAGATVVHSIGGWFAMA AAIMVGPRIDKYNPDGSSNRIGLHNVPLATLGTFFLWFGWFGFNGGSLLRVSVNIGLVIL NTNMAAASAGVSALIFIYATRKRIEAGSLFTAILAGLVAITASSNMVTPVSAVAIGLITG ILAIIAEGFIEKTLKIDDPVSAIAVHGVGGVIGTLCVAIFAQKSYLLAENGSRMHQLGIQ ALGVIVAFSWSFGLGMLFFLCLKKVKRLRVTPEEEKRGLNVAEYEDVASWLDFIRITRLQ DINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSHELRTPLNAIIGFAEVLRDEI AGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTII NASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNS LQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAES TFGKGSTFFFILPINPVNK
experiment.sample.molecule[0].mw30.031
experiment.sample.molecule[0].total_mw30.031
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameKs-Amt5-HK (+10uM ATP)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-03-02
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance2.7
experiment.concentration_min1.0
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.169
experiment.s_max4.423
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw28.6
experimental_mw_errornull
guinier_i0_mw28.6
guinier_i0_mw_error3.0
porod_mw31.0
porod_mw_errornull
pddf_i01801.0
pddf_i0_error6.0
guinier_i01795.0
guinier_i0_error7.0
pddf_rg2.2
pddf_rg_error0.1
guinier_rg2.2
guinier_rg_error0.1
pddf_dmax7.7
pddf_dmax_error0.5
porod_volume53.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last161
pddf_point_first3
pddf_point_last1200
i0_calibration_standard_datanull
intensities_log_log_plotSASDCQ9_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json：36 个字段值
字段路径原始值
codeSASDCQ9
statussuccess
started_at2026-08-11T16:19:48.919627+00:00
finished_at2026-08-11T16:19:57.822556+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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files[0].size6292
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDCQ9",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Signaling ammonium across membranes through an ammonium sensor histidine kinase.",
    "publication": {
      "title": "Signaling ammonium across membranes through an ammonium sensor histidine kinase.",
      "author_list": "Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-017-02637-3",
      "pmid": "29323112",
      "published_date": "2018 Jan 11"
    },
    "status": "released",
    "submitted_date": "2017-11-29",
    "released_date": "2018-01-16"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCQ9.dat",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCQ9.sascif",
  "experiment": {
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        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
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          "long_name": "Ammonium transporter histidine kinase domain",
          "short_name": "Ks-Amt5-HK",
          "sequence": "FIRITRLQDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSAELRTPLNAIIGFAEVLRDEIAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTIINASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNSLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAESTFGKGSTFFFILPINPVNK",
          "organism": "Candidatus Kuenenia stuttgartiensis",
          "uniprot_code": "Q1Q357",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MENIQININHLWVIMAACMVFLMQLGFTSYETGFSQSKNAISIALRNLVDTLISSLVFFS\nVGFGFMFGKSYMGLIGIDLFFANDLALHPNTLSYSFFFFQMVFASTAATILTGAIAERSG\nFIPNIAGTAFIVAIIYPIFGHWAWGNLFSPDQTGWLKELGFIDFAGATVVHSIGGWFAMA\nAAIMVGPRIDKYNPDGSSNRIGLHNVPLATLGTFFLWFGWFGFNGGSLLRVSVNIGLVIL\nNTNMAAASAGVSALIFIYATRKRIEAGSLFTAILAGLVAITASSNMVTPVSAVAIGLITG\nILAIIAEGFIEKTLKIDDPVSAIAVHGVGGVIGTLCVAIFAQKSYLLAENGSRMHQLGIQ\nALGVIVAFSWSFGLGMLFFLCLKKVKRLRVTPEEEKRGLNVAEYEDVASWLDFIRITRLQ\nDINTILEKRVQEKTADLQMANVALEKANRLKSEFLTTMSHELRTPLNAIIGFAEVLRDEI\nAGSLSKDQKEYVTDIHSSGHHLLDMINNILDLSKIETGKMHLQYEEFCIEDAINDTLTII\nNASANNKGISVHTNIQDNTPLLSADKTKFRQILYNLLSNAVKFTPENGKITINVFQKDNS\nLQFEIVDTGIGIKPEDKEKLFEAFHQADASLTREYEGTGLGLHLTKRLVELHGGKIWAES\nTFGKGSTFFFILPINPVNK",
          "mw": 30.031,
          "total_mw": 30.031,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ks-Amt5-HK (+10uM ATP)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
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        "affiliation": [
          {
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            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
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}
查看完整 manifest.json 原文
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