Cytochrome C

{
  "code": "SASDCS8",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions",
    "publication": null,
    "status": "released",
    "submitted_date": "2017-11-02",
    "released_date": "2021-09-20"
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  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    "sample": {
      "molecule": [
        {
          "long_name": "Cytochrome C",
          "short_name": "CytC",
          "sequence": "MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITW\r\nGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE",
          "organism": "Bos taurus",
          "uniprot_code": "P62894",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITW\nGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE",
          "mw": 11.703,
          "total_mw": 11.703,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "TRIS 50mM",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Cytochrome C",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
        ],
        "contributor_name": "Clement",
        "contributor_surname": "Blanchet",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-12-21",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.001,
    "number_of_frames": 1,
    "wavelength": 0.124,
    "sample_detector_distance": 1.6,
    "concentration_min": null,
    "concentration_max": 2.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.035,
    "s_max": 5.57,
    "total_exposure_time": null,
    "seccolumn": null
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  "estimated_volume_method": null,
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  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of cytochrome C in 50 mM Tris, pH 7.4 were collected at the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using an Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. A single 1.3 millisecond frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled to the protein concentration.",
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    "benchmark"
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  "guinier_rg": 1.34,
  "guinier_rg_error": 0.07,
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  "estimated_volume_error": null,
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  "guinier_point_last": 401,
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  "symmetry": null,
  "last_modified": "2023-06-21T07:55:58.257585+02:00",
  "bragg_peak": []
}

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