SASDCT5

SAXS on intact Doryteuthis squid lens 40% layer

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2022-01-27T08:12:18.914214+01:00

The space-filling model ultimately represents the spatial density fluctuation of the material, not the presence of material. Since we measured a nearly water-free density of material at the core of the lens, we show the void density of the model prediction here.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · SAXS on intact Doryteuthis squid lens 40% layer

浓度— – — 缓冲液 / pHTissue fluid / 7.0
实验温度10.0 设备 / 束线National Synchrotron Light Source (NSLS) / X9A
波长0.1 nm曝光300.0 s × 1

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
S-crystallin, Q25367_DOROP
查看序列
MPTYTLYYFNGRGRAEVLRMMFAAANVKYMDKRFEFNEWDKYRKDMPSMCVPVLEMDGGNKMPETMAIPRYLAREFGFYGKSNMDMMRCDYIADCFYEIMHDYMRYFHEKNGRFRSSMEGSGNGMNSPTGASGDMNSNSNFDTYLQWRFMNTCNRVLPFLERTLEMQNGGKAFFMGDQMMWCDMMCHCALEGPFMENQSMLSKHPKLMALRTRIASHPKISGYLKSRSNTNW
proteinDoryteuthis pealeiiQ253671–232dimer分子数 227.289 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11993[2]0.004–1.0 1/A无误差列缺失 19930sasbdb/entries/t5/sasdct5/source/SASDCT5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
i0P(r)180.0——
mwExperimental50.0—kDa
rgP(r)2.4—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称Tissue fluid缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2014-07-17储存 / 测量温度10.0 / 10.0
曝光时间300.0帧数1
波长0.1样品-探测器距离—
光源X-ray synchrotron探测器Pilatus 300K
机构 / 束线National Synchrotron Light Source (NSLS) / X9A · Brookhaven, NY, USA
q 范围0.04 – 10.0样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Kratky 图
Kratky 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/t5/sasdct5/source/SASDCT5.outnot_listed——下载查看原文件
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summarysasbdb/entries/t5/sasdct5/source/summary.jsondownloaded8309d37ccb457b6bbb2f543bd9abd3dad048ad41b2060df9806866bffde00ce99239下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(5 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDCT5
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleEye patches: Protein assembly of index-gradient squid lenses.
project.publication.titleEye patches: Protein assembly of index-gradient squid lenses.
project.publication.author_listCai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM
project.publication.journalScience
project.publication.doi10.1126/science.aal2674
project.publication.pmid28798124
project.publication.published_date2017 Aug 11
project.statusreleased
project.submitted_date2017-06-29
project.released_date2017-08-18
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descriptionThe space-filling model ultimately represents the spatial density fluctuation of the material, not the presence of material. Since we measured a nearly water-free density of material at the core of the lens, we show the void density of the model prediction here.
experiment_descriptionThis dataset is SAXS characterization of the core region (tissue from radial positions of 40%–0% of the total lens radius) of the intact Doryteuthis squid eye lens tissue. The lens contains about 40 unique S-crystallins isoforms that vary in molecular weight due to length variation in a disordered region encoded in the central exon of the coding sequence such that loops form protruding from the folded protein. S-crystallins are dimeric and globular, such that each dimer has two of these disordered loops, which form linking structures between particles in the system. Because the proteins link to form extended materials, even at very low concentration, the parameter Rg does not apply here. Similarly, because this is a measurement of intact biological tissue, there is no experimental buffer per se to subtract, and accordingly, raw data without buffer subtraction are reported here. The pair-distance distribution function (p(r)) was calculated based on unsubtracted data (refer to the full entry zip archive for the p(r) profile). The full set of sequences describing the protein polydispersity in this system is available at GenBank BioProject PRJNA386124. Synchrotron SAXS data were collected on the X9A beam line of the National Synchrotron Light Source (NSLS; Brookhaven, NY, USA) using a 20Hz Pilatus 300K detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). The sample measurement (1 x 300 s exposure) was performed at at 10°C.
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last_modified2022-01-27T08:12:18.914214+01:00
bragg_peak[]
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字段路径原始值
codeSASDCT5
statussuccess
started_at2026-08-11T14:55:33.187479+00:00
finished_at2026-08-11T14:55:43.380327+00:00
source_last_modified2022-01-27T08:12:18.914214+01:00
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查看完整 summary.json 原文
{
  "code": "SASDCT5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Eye patches: Protein assembly of index-gradient squid lenses.",
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      "title": "Eye patches: Protein assembly of index-gradient squid lenses.",
      "author_list": "Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM",
      "journal": "Science",
      "doi": "10.1126/science.aal2674",
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  "description": "The space-filling model ultimately represents the spatial density fluctuation of the material, not the presence of material.  Since we measured a nearly water-free density of material at the core of the lens, we show the void density of the model prediction here.",
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  "last_modified": "2022-01-27T08:12:18.914214+01:00",
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}
查看完整 manifest.json 原文
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