SASDCV7

TET12(2.3)SN-f5b

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-06-21T07:56:18.120449+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · TET12(2.3)SN-f5b

浓度1.1 – 4.1 缓冲液 / pH20 mM Tris 150 mM NaCl 10% glycerol / 7.5
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.045 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
TET12(2.3)SN-f5b
查看序列
MSPEDENSQLEEKISQLKQKNSELKEEIQQLEYGSGPGSPEDEIQQLEEKNSQLKQEISQLEEKNQELKYGSGPGLEEELKQLEEELQAIEEQLAQLQWKAQARKEKLAQLKEKLGSGPGLEQIEERLEQIEERLQAKEWEKAQLREELQALREKLAQLGSGPGSPEDKNSELKEEIQQLEEENQQLEEKISELKYGSGPGDIEQELERAKESIRRLEQEVNQERSRMQYLQTLLEKGSGPGLEEELKQLEEELQAIEEQLAQLQWKAQARKEKLAQLKEKLGSGPGSPEDEIQQLEEEISQLEQKNSELKEKNQELKYGSGPGLEQIEERLEQIEERLQAKEWEKAQLREELQALREKLAQLGSGPGDIEQELERAKESIRRLEQEVNQERSRMQYLQTLLEKGSGPGSPEDKISELKEENQQLEQKIQQLKEENSQLEYGSGPGSPEDKISQLKEKIQQLKQENQQLEEENSQLEYGLEHHHHHHHH
proteinsynthetic construct——–—monomer分子数 156.682 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11205[3]0.154492–6.715565 1/nm含误差列缺失 01sasbdb/entries/v7/sasdcv7/source/SASDCV7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)11.471.0nm
i0Guinier6577.518.0—
i0P(r)6716.022.0—
mwExperimental50.0—kDa
mwGuinier I(0)50.01.5kDa
mwPorod61.7—kDa
porod_volumePorod105.0—nm³
rgGuinier3.450.2nm
rgP(r)3.530.01nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris 150 mM NaCl 10% glycerol缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-06-10储存 / 测量温度10.0 / 20.0
曝光时间0.045帧数20
波长0.124样品-探测器距离2.0
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.154 – 6.716样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/v7/sasdcv7/source/SASDCV7.zipdownloaded1264949b3a2ce095bcde8ed748225a23611b5f645d54a66b8f3943c849804cb3862245下载查看原文件源站
pddfsasbdb/entries/v7/sasdcv7/source/SASDCV7.outdownloaded44609a812301aacc0e901af6aaffe88e4248b25881b1d880b378afdfdda1c9c783d51下载查看原文件源站
sascifsasbdb/entries/v7/sasdcv7/source/SASDCV7.sascifdownloaded576225da10a19a6a00c7e289a586ff03f370eede8318a7e0d46853f9af0b37feabc834下载查看原文件源站
summarysasbdb/entries/v7/sasdcv7/source/summary.jsondownloaded8125e657194dd3e00f5c65019410d6cbd5a9f3d477983e8dfbfeb387c6c3a1751619下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDCV7
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleDesign of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.
project.publication.titleDesign of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.
project.publication.author_listLjubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
project.publication.journalNat Biotechnol
project.publication.doi10.1038/nbt.3994
project.publication.pmid29035374
project.publication.published_date2017 Nov
project.statusreleased
project.submitted_date2017-09-28
project.released_date2017-10-09
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experiment.instrument.detector.typeCustom-built
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experiment.instrument.detector.resolution0.172
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometryPoint
experiment.instrument.type_of_sourceX-ray synchrotron
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experiment.sample.molecule[0].organismsynthetic construct
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw56.682
experiment.sample.molecule[0].total_mw56.682
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 150 mM NaCl 10% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
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experiment.sample.purity_methodnull
experiment.sample.nameTET12(2.3)SN-f5b
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameNational Research Council
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameJana
experiment.contributor[0].contributor_surnameAupič
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-06-10
experiment.storage_temperature10.0
experiment.cell_temperature20.0
experiment.exposure_time0.045
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance2.0
experiment.concentration_min1.1
experiment.concentration_max4.1
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.154
experiment.s_max6.716
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDCV7_fit1_model1_img.png
fits[0].models[0].softwareMODELLER
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_version9.16
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fits[0].models[0].model_mw56.63
fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
fits[0].models[0].symmetry
fits[0].models[0].comment
fits[0].models[0].user201
fits[0].fit_unit1/A
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fits[0].softwareOther
fits[0].chi_square_value1.3
fits[0].p_value0.0
fits[0].fit_residual_plotSASDCV7_fit1_fitresiduals_img.png
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fits[0].software_versionPepsiSAXS
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of TET12(2.3)SN-f5b in 20 mM Tris 150 mM NaCl 10% glycerol, pH 7.5 were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.1 and 4.1 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. To detect any potential concentration dependent effects a series consisting of 3 concentrations was measured. The highest concentration was 4.1 mg/ml, while the lowest concentrations was 1.1 mg/ml. Frames were carefully inspected for radiation damage and those not showing any radiation damage were then averaged. Before and after each sample scattering, matching buffer was measured and subtracted from sample scattering. Scattering curves were merged and analyzed using PRIMUS software.
tags[]
intensity_unitnull
experimental_mw50.0
experimental_mw_errornull
guinier_i0_mw50.0
guinier_i0_mw_error1.5
porod_mw61.7
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pddf_i06716.0
pddf_i0_error22.0
guinier_i06577.5
guinier_i0_error18.0
pddf_rg3.53
pddf_rg_error0.01
guinier_rg3.45
guinier_rg_error0.2
pddf_dmax11.47
pddf_dmax_error1.0
porod_volume105.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first17
guinier_point_last41
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCV7_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:18.120449+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCV7
statussuccess
started_at2026-08-11T16:45:48.448410+00:00
finished_at2026-08-11T16:46:01.753915+00:00
source_last_modified2023-06-21T07:56:18.120449+02:00
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查看完整 summary.json 原文
{
  "code": "SASDCV7",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.",
    "publication": {
      "title": "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.",
      "author_list": "Ljubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R",
      "journal": "Nat Biotechnol",
      "doi": "10.1038/nbt.3994",
      "pmid": "29035374",
      "published_date": "2017 Nov"
    },
    "status": "released",
    "submitted_date": "2017-09-28",
    "released_date": "2017-10-09"
  },
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      "beamline_name": "EMBL P12",
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查看完整 manifest.json 原文
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