SASDCY6

T2 in b-DDM micelle (SEC-SAXS)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · T2 in b-DDM micelle (SEC-SAXS)

浓度— – — 缓冲液 / pH20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM / 7.5
实验温度10.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光1.0 s × 3600

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Mechanosensitive channel T2
查看序列
MDRAKGKSIAVYVGSMIVLIIVLLALLYVLSLFHIVPIKFSRILYAVVIGLVIYAIVRIVTKYVERFISLHSEFKHLKYIVFIISLLGYFIISLAVLAALGIDVSSVILGSAFLSAIIGLAAQSVLANVFGGLFLSIVRPFKLGDHIVVNTWQFPVSFPSYPPKYFSRDFIEGSIYRGIVSDISINYTSIALESGDVVKIPNNIMIQAAITLRHGSVTVQARYEVPKYINFNDIYNKIVDEVGTLPGARNIGVMIDETTVNTYIILVRGDFQGEDADLVRSQILQKVIGIVEPLKKQ
proteinThermoplasma volcaniumQ97AH51–197heptamer分子数 732.887 kDa
n-Dodecyl-β-D-Maltopyranosideothersynthetic construct——–—other分子数 —0.511 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11662[3]0.0778114–4.60696 1/nm含误差列缺失 0164sasbdb/entries/y6/sasdcy6/source/SASDCY6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)16.50.5nm
i0Guinier251.05.0arbitrary
i0P(r)250.05.0arbitrary
mwExperimental355.040.0kDa
mwPorod560.060.0kDa
porod_volumePorod896.090.0nm³
rgGuinier5.60.1nm
rgP(r)5.60.2nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-05-29储存 / 测量温度10.0 / 10.0
曝光时间1.0帧数3600
波长0.124样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.078 – 4.607样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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summarysasbdb/entries/y6/sasdcy6/source/summary.jsondownloaded75776b9ea4f828730bc4335097935fb3040a51357ddf1202431b029df3ce58c57fed下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:173 个字段值
字段路径原始值
codeSASDCY6
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleSaposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
project.publication.titleSaposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
project.publication.author_listFlayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
project.publication.journalStructure
project.publication.doi10.1016/j.str.2018.01.007
project.publication.pmid29413323
project.publication.published_date2018 Feb 6
project.statusreleased
project.submitted_date2017-08-09
project.released_date2018-02-07
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMechanosensitive channel T2
experiment.sample.molecule[0].short_nameT2
experiment.sample.molecule[0].sequenceMDRAKGKSIAVYVGSMIVLIIVLLALLYVLSLFHIVPIKFSRILYAVVIGLVIYAIV RIVTKYVERFISLHSEFKHLKYIVFIISLLGYFIISLAVLAALGIDVSSVILGSAFL SAIIGLAAQSVLANVFGGLFLSIVRPFKLGDHIVVNTWQFPVSFPSYPPKYFSRDFI EGSIYRGIVSDISINYTSIALESGDVVKIPNNIMIQAAITLRHGSVTVQARYEVPKY INFNDIYNKIVDEVGTLPGARNIGVMIDETTVNTYIILVRGDFQGEDADLVRSQILQ KVIGIVEPLKKQ
experiment.sample.molecule[0].organismThermoplasma volcanium
experiment.sample.molecule[0].uniprot_codeQ97AH5
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last197
experiment.sample.molecule[0].oligomerizationheptamer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].total_mw230.209
experiment.sample.molecule[0].number_molecules7
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_namen-Dodecyl-β-D-Maltopyranoside
experiment.sample.molecule[1].short_nameb-DDM
experiment.sample.molecule[1].sequencenull
experiment.sample.molecule[1].organismsynthetic construct
experiment.sample.molecule[1].uniprot_codenull
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experiment.sample.molecule[1].uniprot_range_lastnull
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experiment.sample.molecule[1].molecular_typeother
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw0.511
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experiment.sample.molecule[1].number_molecules0
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
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experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameT2 in b-DDM micelle (SEC-SAXS)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-05-29
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time1.0
experiment.number_of_frames3600
experiment.wavelength0.124
experiment.sample_detector_distance3.1
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experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.078
experiment.s_max4.607
experiment.total_exposure_time3600.0
experiment.seccolumnnull
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fits[0].chi_square_value3.4
fits[0].p_value0.0
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pddf_softwareATSAS GNOM
pddf_software_version2.9.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw355.0
experimental_mw_error40.0
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw560.0
porod_mw_error60.0
pddf_i0250.0
pddf_i0_error5.0
guinier_i0251.0
guinier_i0_error5.0
pddf_rg5.6
pddf_rg_error0.2
guinier_rg5.6
guinier_rg_error0.1
pddf_dmax16.5
pddf_dmax_error0.5
porod_volume896.0
porod_volume_error90.0
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last30
pddf_point_first1
pddf_point_last1000
i0_calibration_standard_datanull
intensities_log_log_plotSASDCY6_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCY6
statussuccess
started_at2026-08-11T15:18:38.816379+00:00
finished_at2026-08-11T15:18:48.452038+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDCY6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.",
    "publication": {
      "title": "Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.",
      "author_list": "Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C",
      "journal": "Structure",
      "doi": "10.1016/j.str.2018.01.007",
      "pmid": "29413323",
      "published_date": "2018 Feb 6"
    },
    "status": "released",
    "submitted_date": "2017-08-09",
    "released_date": "2018-02-07"
  },
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          "long_name": "Mechanosensitive channel T2",
          "short_name": "T2",
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查看完整 manifest.json 原文
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