SASDCZ3

Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 2.3 mg/mL

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-05-18T10:06:39.622484+02:00

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 2.3 mg/mL

Concentration	— – 2.3	Buffer / pH	50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol / 7.8
Experimental temperature	—	Facility / beamline	Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength	— nm	Exposure	— s × —

Molecular Components

Component	Type / organism	UniProt and Construct	Oligomeric State	Molecular weight
Proline dehydrogenase View sequence GHMPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKEDDRLGGVEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQALERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKLSALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPSLKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQRLHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPVPALLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLKPIADATPDQAHAAVAAARAGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGKTLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTGESNALTMRGRGVFVAISPWNFPLAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGAALTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDVVTSAFRSAGQRCSALRLLFVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAKQRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEAGQLTEEVFGPILHVVRYRPENLERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNGLSGTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE	proteinBradyrhizobium diazoefficiens	Q89E26—–—	dimerMolecule count 2	107.531 kDa

Experimental Curves

Curve	Points / columns	q range	Uncertainty quality	Negative-intensity points	Source file
1	513[3]	0.013075–0.324398 1/A	Has uncertainty columnMissing 0	0	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.dat

▼

2. Measurements Reported by SASBDB Reported Results

Metric	Method	Value	Uncertainty	Unit
dmax	P(r)	14.5	—	Å
i0	Guinier	118.9	0.43	—
i0	P(r)	116.0	0.5	—
mw	Experimental	216.0	—	kDa
porod_volume	Porod	281.0	—	Å³
rg	Guinier	4.49	0.07	Å
rg	P(r)	4.55	0.01	Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

Category	Path	Download status	Size
curve	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.dat	downloaded	23085
full_entry_zip	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.zip	downloaded	19766
pddf	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.out	downloaded	47904
sascif	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.sascif	downloaded	61913
summary	sasbdb/entries/z3/sasdcz3/source/summary.json	downloaded	8167

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name	50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol	Buffer concentration	—
pH	7.8	Additives	—
Buffer description	BjPutA buffer
Purity measurement method	—	Extinction coefficient	—
Absorbance	—	Scattering contrast	—
Specific volume / dry volume	— / —	Mixture / deuteration	— / —

Acquisition Conditions and Instrument

Measurement date	2016-12-16	Storage / measurement temperature	— / —
Exposure time	—	Frames	—
Wavelength	—	Sample-to-detector distance	—
Source	X-ray synchrotron	Detector	Pilatus3 X 2M
Facility / beamline	Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range	0.131 – 3.244	Sample volume / flow rate	— / —

Original SASBDB Plots

Downloadable Files

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full_entry_zip	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.zip	downloaded	19766	72be538fb0efcfa42d4848bbdba5e008f85af88430c21c9bfa5a8025338c88dc	DownloadView source fileSource site
pddf	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.out	downloaded	47904	10852a3bf92a98d457f1ade90c6dcf666f9ce87bd549abe1781c6caeae3787b4	DownloadView source fileSource site
sascif	sasbdb/entries/z3/sasdcz3/source/SASDCZ3.sascif	downloaded	61913	2067935f5b017ea6ff3357211d1f8c3463298d6106a123afd6d5832348e38474	DownloadView source fileSource site
summary	sasbdb/entries/z3/sasdcz3/source/summary.json	downloaded	8167	8d18da654407d0a2a73a521f32d0c475c8815e63c436bc4f373515c9fba9763d	DownloadView source fileSource site

curve：source record

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full_entry_zip：source record and ZIP contents (2 items)

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pddf：source record

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sascif：source record

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summary：source record

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：134 field values

Field path	Raw value
code	SASDCZ3
status	Published
type_of_curve	Single concentration
angular_unit	1/A
project.title	Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.
project.publication.title	Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.
project.publication.author_list	Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
project.publication.journal	FEBS J
project.publication.doi	10.1111/febs.14165
project.publication.pmid	28710792
project.publication.published_date	2017 Sep
project.status	released
project.submitted_date	2017-05-19
project.released_date	2017-07-17
pddf_data	https://www.sasbdb.org/media/p_of_R_files/SASDCZ3.out
intensities_data	https://www.sasbdb.org/media/intensities_files/SASDCZ3.dat
intensities_log_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ3_dat_img.png
intensities_kratky_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ3_kratky_img.png
pddf_plot	https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCZ3_pofr_img.png
intensities_guinier_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ3_guinier_img.png
sascif_data	https://www.sasbdb.org/media/sascif/sascif_files/SASDCZ3.sascif
experiment.instrument.detector.type	null
experiment.instrument.detector.name	Pilatus3 X 2M
experiment.instrument.detector.resolution	172.0
experiment.instrument.name	Advanced Light Source (ALS)
experiment.instrument.city	Berkeley, CA
experiment.instrument.country	USA
experiment.instrument.beamline_name	12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_source	X-ray synchrotron
experiment.instrument.point_source	null
experiment.instrument.line_collimation	null
experiment.instrument.sample_path_length	null
experiment.instrument.line_collimation_slitlength	null
experiment.instrument.line_collimation_integrationwidth	null
experiment.instrument.xray_energy	null
experiment.instrument.beam_profile_ah	null
experiment.instrument.beam_profile_al	null
experiment.sample.molecule[0].long_name	Proline dehydrogenase
experiment.sample.molecule[0].short_name	BjPutA R51E
experiment.sample.molecule[0].sequence	GHMPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKEDDRLGGVEDMLREFALSTKEGLALMVL AEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQ AMRLMGNHFVLGETIEQALERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKL SALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPSLKGWDGFGLAIQAYQKRA SAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNAL TVATVLEMAEGSSGFEFQRLHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPV PALLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLKPIADATPDQAHAAVAAAR AGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGKTLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTG ESNALTMRGRGVFVAISPWNFPLAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGA ALTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDVVTSAFRSAGQRCSALRLL FVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAKQRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEA GQLTEEVFGPILHVVRYRPENLERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNG LSGTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE
experiment.sample.molecule[0].organism	Bradyrhizobium diazoefficiens
experiment.sample.molecule[0].uniprot_code	Q89E26
experiment.sample.molecule[0].uniprot_range_first	null
experiment.sample.molecule[0].uniprot_range_last	null
experiment.sample.molecule[0].oligomerization	dimer
experiment.sample.molecule[0].molecular_type	protein
experiment.sample.molecule[0].uniprot_sequence	MPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRDDRLGGVED MLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASA WALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQALERG KPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKLSA LHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPS LKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDG YPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQRLHG MGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPVPA LLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLK PIADATPDQAHAAVAAARAGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGK TLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTGESNALTMRGRGVFVAISPWNFP LAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGAAL TAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDV VTSAFRSAGQRCSALRLLFVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAK QRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEAGQLTEEVFGPILHVVRYRPENL ERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNGLS GTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE
experiment.sample.molecule[0].mw	107.531
experiment.sample.molecule[0].total_mw	215.062
experiment.sample.molecule[0].number_molecules	2
experiment.sample.molecule[0].complex_state	False
experiment.sample.molecule[0].deuteration	null
experiment.sample.molecule[0].molecule_source	biological
experiment.sample.molecule[0].molecule_description	null
experiment.sample.buffer.name	50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol
experiment.sample.buffer.concentration_unit	null
experiment.sample.buffer.comment	BjPutA buffer
experiment.sample.buffer.additive	null
experiment.sample.buffer.concentration	null
experiment.sample.buffer.pka	null
experiment.sample.buffer.ph	7.8
experiment.sample.buffer.deuteration	null
experiment.sample.purity_method	null
experiment.sample.name	Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 2.3 mg/mL
experiment.sample.ext_coefficient	null
experiment.sample.contrast	null
experiment.sample.specific_vol	null
experiment.sample.dry_vol	null
experiment.sample.absorbption	null
experiment.sample.deuteration	null
experiment.sample.mixture	null
experiment.contributor[0].affiliation[0].short_name	Mizzou
experiment.contributor[0].affiliation[0].address	Columbia, MO, USA
experiment.contributor[0].affiliation[0].full_name	University of Missouri-Columbia
experiment.contributor[0].affiliation[0].webpage	http://missouri.edu/
experiment.contributor[0].contributor_name	John
experiment.contributor[0].contributor_surname	Tanner
experiment.contributor[0].orcid	null
experiment.concentration_method	null
experiment.concentration_unit	null
experiment.date	2016-12-16
experiment.storage_temperature	null
experiment.cell_temperature	null
experiment.exposure_time	null
experiment.number_of_frames	null
experiment.wavelength	null
experiment.sample_detector_distance	null
experiment.concentration_min	null
experiment.concentration_max	2.3
experiment.sample_volume	null
experiment.flow_rate	null
experiment.s_min	0.131
experiment.s_max	3.244
experiment.total_exposure_time	null
experiment.seccolumn	null
fits	[]
estimated_volume_method	null
pddf_software	ATSAS GNOM
pddf_software_version	null
i0_calibration_standard	null
description	null
experiment_description	Synchrotron SAXS data from solutions of Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant in 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH 7.8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.3 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged. The radially averaged scattering of the solvent-blank was subtracted. X-ray wavelength, unknown; Exposure time, unknown; Sample-To-Detector distance, unknown.
tags	[]
intensity_unit	null
experimental_mw	216.0
experimental_mw_error	null
guinier_i0_mw	null
guinier_i0_mw_error	null
porod_mw	null
porod_mw_error	null
pddf_i0	116.0
pddf_i0_error	0.5
guinier_i0	118.9
guinier_i0_error	0.43
pddf_rg	4.55
pddf_rg_error	0.01
guinier_rg	4.49
guinier_rg_error	0.07
pddf_dmax	14.5
pddf_dmax_error	null
porod_volume	281.0
porod_volume_error	null
estimated_volume	null
estimated_volume_error	null
guinier_point_first	1
guinier_point_last	27
pddf_point_first	null
pddf_point_last	null
i0_calibration_standard_data	null
intensities_log_log_plot	SASDCZ3_datloglog_img.png
symmetry	null
last_modified	2026-05-18T10:06:39.622484+02:00
bragg_peak	[]

manifest.json：36 field values

Field path	Raw value
code	SASDCZ3
status	success
started_at	2026-08-11T14:11:29.550752+00:00
finished_at	2026-08-11T14:11:38.106373+00:00
source_last_modified	2026-05-18T10:06:39.622484+02:00
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View the complete summary.json source

{
  "code": "SASDCZ3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.",
    "publication": {
      "title": "Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.",
      "author_list": "Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ",
      "journal": "FEBS J",
      "doi": "10.1111/febs.14165",
      "pmid": "28710792",
      "published_date": "2017 Sep"
    },
    "status": "released",
    "submitted_date": "2017-05-19",
    "released_date": "2017-07-17"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCZ3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCZ3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCZ3_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ3_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCZ3.sascif",
  "experiment": {
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      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
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        {
          "long_name": "Proline dehydrogenase",
          "short_name": "BjPutA R51E",
          "sequence": "GHMPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKEDDRLGGVEDMLREFALSTKEGLALMVL\r\nAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQ\r\nAMRLMGNHFVLGETIEQALERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKL\r\nSALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPSLKGWDGFGLAIQAYQKRA\r\nSAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNAL\r\nTVATVLEMAEGSSGFEFQRLHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPV\r\nPALLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLKPIADATPDQAHAAVAAAR\r\nAGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGKTLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTG\r\nESNALTMRGRGVFVAISPWNFPLAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGA\r\nALTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDVVTSAFRSAGQRCSALRLL\r\nFVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAKQRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEA\r\nGQLTEEVFGPILHVVRYRPENLERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNG\r\nLSGTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE",
          "organism": "Bradyrhizobium diazoefficiens",
          "uniprot_code": "Q89E26",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRDDRLGGVED\nMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASA\nWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQALERG\nKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKLSA\nLHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPS\nLKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDG\nYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQRLHG\nMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPVPA\nLLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLK\nPIADATPDQAHAAVAAARAGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGK\nTLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTGESNALTMRGRGVFVAISPWNFP\nLAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGAAL\nTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDV\nVTSAFRSAGQRCSALRLLFVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAK\nQRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEAGQLTEEVFGPILHVVRYRPENL\nERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNGLS\nGTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE",
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          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
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        "name": "50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol",
        "concentration_unit": null,
        "comment": "BjPutA buffer",
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        "deuteration": null
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      "name": "Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 2.3 mg/mL",
      "ext_coefficient": null,
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      "absorbption": null,
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    "contributor": [
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        "affiliation": [
          {
            "short_name": "Mizzou",
            "address": "Columbia, MO, USA",
            "full_name": "University of Missouri-Columbia",
            "webpage": "http://missouri.edu/"
          }
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        "contributor_name": "John",
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        "orcid": null
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    "date": "2016-12-16",
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  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant in 50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH 7.8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.3 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged. The  radially averaged scattering of the solvent-blank was subtracted.  X-ray wavelength, unknown; Exposure time, unknown; Sample-To-Detector distance, unknown.",
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  "intensities_log_log_plot": "SASDCZ3_datloglog_img.png",
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  "last_modified": "2026-05-18T10:06:39.622484+02:00",
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View the complete manifest.json source

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Title	Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.
Authors	Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
DOI / PMID	10.1111/febs.14165 / 28710792