SASDD32

DNA-(adenine N6)-methyltransferase from Acinetobacter baumannii ATCC 17978

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00

The individual DAMMIN models used to generate that averaged spatial representation of the protein (DAMFILT model) are included in the full entry zip archive as well as the refinement of the averaged structure using DAMSTART.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · DNA-(adenine N6)-methyltransferase from Acinetobacter baumannii ATCC 17978

Concentration— – 2.4 Buffer / pH150mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerol / 7.4
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
DNA-(adenine N6)-methyltransferase
View sequence
MNSEPSVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYRVIANDWEPYSHALNHAILACVDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDVYDPSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQGWGGRTQTALERIESLLWLTPSRFCEIGDRKRPAAEMWCVDAQHLANQMSGFEVDVAYLDPPYNQHAYSSNYHVLNALTLWDQVDLPPPDTKGYKSGIDRAWRKERPSPYNSSKHAKDAYEKLLSTINARYILTSYSTDGNIEPKDLLMANLERGKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKPGPPLRQLLGQLYHFAELGGVDTSGNSTQLALW
proteinAcinetobacter baumannii—2–430monomerMolecule count 148.902 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11809[3]0.0133796–3.7925 1/nmHas uncertainty columnMissing 015sasbdb/entries/32/sasdd32/source/SASDD32.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.11—nm
i0Guinier3765.766.6arbitrary
i0P(r)3773.08.0arbitrary
mwExperimental52.0—kDa
mwGuinier I(0)52.0—kDa
porod_volumePorod96.0—nm³
rgGuinier2.910.31nm
rgP(r)2.970.01nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name150mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerolBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-09-30Storage / measurement temperature10.0 / 10.0
Exposure time0.05Frames20
Wavelength0.124Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.013 – 3.792Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (7 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:169 field values
Field pathRaw value
codeSASDD32
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleRecombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity.
project.publication.titleRecombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity.
project.publication.author_listBlaschke U, Suwono B, Zafari S, Ebersberger I, Skiebe E, Jeffries CM, Svergun DI, Wilharm G
project.publication.journalProtein Expr Purif
project.publication.doi10.1016/j.pep.2018.06.009
project.publication.pmid29908915
project.publication.published_date2018 Nov
project.statusreleased
project.submitted_datenull
project.released_date2018-06-22
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDD32.out
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pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD32_pofr_img.png
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDD32.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDNA-(adenine N6)-methyltransferase
experiment.sample.molecule[0].short_nameA1S_0222
experiment.sample.molecule[0].sequenceMNSEPSVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVV SRLARQNGYRVIANDWEPYSHALNHAILACVDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDV YDPSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQGWGGR TQTALERIESLLWLTPSRFCEIGDRKRPAAEMWCVDAQHLANQMSGFEVDVAYLDPPYNQHAYSSNYHVL NALTLWDQVDLPPPDTKGYKSGIDRAWRKERPSPYNSSKHAKDAYEKLLSTINARYILTSYSTDGNIEPK DLLMANLERGKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKPGPPLRQLLGQLYHFAELGGVDT SGNSTQLALW
experiment.sample.molecule[0].organismAcinetobacter baumannii
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last430
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw48.902
experiment.sample.molecule[0].total_mw48.902
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name150mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDNA-(adenine N6)-methyltransferase from Acinetobacter baumannii ATCC 17978
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameCy M
experiment.contributor[0].contributor_surnameJeffries
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-09-30
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max2.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.013
experiment.s_max3.792
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIN
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fits[0].models[0].bead_radius2.75
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fits[0].models[0].commentnull
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fits[0].softwareDAMMIN
fits[0].chi_square_value1.062
fits[0].p_value0.132008
fits[0].fit_residual_plotSASDD32_fit1_fitresiduals_img.png
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe individual DAMMIN models used to generate that averaged spatial representation of the protein (DAMFILT model) are included in the full entry zip archive as well as the refinement of the averaged structure using DAMSTART.
experiment_descriptionSynchrotron SAXS data from solutions of DNA-(adenine N6)-methyltransferase in 150mM NaCl, 10mM Tris, 1mM DTT, 5% v/v glycerol, pH 7.4 were collected at the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled to protein concentration.
tags[]
intensity_unitarbitrary
experimental_mw52.0
experimental_mw_errornull
guinier_i0_mw52.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i03773.0
pddf_i0_error8.0
guinier_i03765.76
guinier_i0_error6.6
pddf_rg2.97
pddf_rg_error0.01
guinier_rg2.91
guinier_rg_error0.31
pddf_dmax11.11
pddf_dmax_errornull
porod_volume96.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first30
guinier_point_last208
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDD32_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDD32
statussuccess
started_at2026-08-11T13:29:23.629285+00:00
finished_at2026-08-11T13:29:33.397953+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDD32",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity.",
    "publication": {
      "title": "Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity.",
      "author_list": "Blaschke U, Suwono B, Zafari S, Ebersberger I, Skiebe E, Jeffries CM, Svergun DI, Wilharm G",
      "journal": "Protein Expr Purif",
      "doi": "10.1016/j.pep.2018.06.009",
      "pmid": "29908915",
      "published_date": "2018 Nov"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2018-06-22"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDD32.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDD32.dat",
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  "experiment": {
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        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "DNA-(adenine N6)-methyltransferase",
          "short_name": "A1S_0222",
          "sequence": "MNSEPSVYHKRRHAARTTDEYLFHQLVPYLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVV\r\nSRLARQNGYRVIANDWEPYSHALNHAILACVDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDV\r\nYDPSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQGWGGR\r\nTQTALERIESLLWLTPSRFCEIGDRKRPAAEMWCVDAQHLANQMSGFEVDVAYLDPPYNQHAYSSNYHVL\r\nNALTLWDQVDLPPPDTKGYKSGIDRAWRKERPSPYNSSKHAKDAYEKLLSTINARYILTSYSTDGNIEPK\r\nDLLMANLERGKVTLLTQDVPRYRVSKQRQSERARVLEFIVITDTHAKPGPPLRQLLGQLYHFAELGGVDT\r\nSGNSTQLALW",
          "organism": "Acinetobacter baumannii",
          "uniprot_code": null,
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            "short_name": "EMBL-Hamburg",
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}
View the complete manifest.json source
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