查看完整 summary.json 原文
{
"code": "SASDD45",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA.",
"publication": {
"title": "The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA.",
"author_list": "Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP",
"journal": "J Biol Chem",
"doi": "10.1074/jbc.RA118.005173",
"pmid": "30337369",
"published_date": "2018 Oct 18"
},
"status": "released",
"submitted_date": "2018-03-17",
"released_date": "2018-11-03"
},
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"name": "Diamond Light Source",
"city": "Didcot",
"country": "UK",
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{
"long_name": "Type II toxin-antitoxin system HicB family antitoxin",
"short_name": "HicB",
"sequence": "MMEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEF\r\nTCSTVEELVAKPEYAGAVWALVSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRS\r\nGFLARAALEALNEGKKHHHHHH",
"organism": "Burkholderia pseudomallei",
"uniprot_code": "Q63NA5",
"uniprot_range_first": 1,
"uniprot_range_last": 134,
"oligomerization": "tetramer",
"molecular_type": "protein",
"uniprot_sequence": "MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEFT\nCSTVEELVAKPEYAGAVWALVSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSG\nFLARAALEALNEGKVRHA",
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"number_molecules": 4,
"complex_state": false,
"deuteration": null,
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}
],
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"pka": null,
"ph": 7.5,
"deuteration": null
},
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"name": "Type II toxin-antitoxin system HicB family antitoxin - HicB protein",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Bristol, UK",
"full_name": "University of Bristol",
"webpage": "http://www.bristol.ac.uk"
}
],
"contributor_name": "Ash",
"contributor_surname": "Winter",
"orcid": null
}
],
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"concentration_unit": null,
"date": "2016-07-28",
"storage_temperature": 4.0,
"cell_temperature": 25.0,
"exposure_time": 3.0,
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"pdb_code": "6g1n",
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}
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"description": "Analysis of the data involved ScÅtter (www.bioisis.net/). The resultant curve was fit with the crystal structure of HicB, PDB Code: 6G1N.",
"experiment_description": "Synchrotron SAXS data from solutions of HicB protein in 25 mM Tris 150 mM NaCl, pH 7.5, were collected using in-line SEC-SAXS at the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In line SEC-SAXS was performed using an Agilent 1200 HPLC system equipped with a 2.4 mL Superdex S200 column (GE Healthcare). A sample concentration of 5mg/ml was loaded onto the column at a flow rate of 0.04 mL/min. Data frames were collected at 25°C for the entire column elution with an exposure time of 3 seconds per frame through an angular s-range of 0.04-4 nm-1. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted using data frames measured from the flow-through column buffer with no macromolecular sample present.",
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"guinier_point_first": 35,
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"pddf_point_first": null,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDD45_datloglog_img.png",
"symmetry": null,
"last_modified": "2020-05-08T08:19:46.838764+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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