SASDD95

Neurexin 1a L5L6

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2021-08-18T17:28:22.895829+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Neurexin 1a L5L6

Concentration2.0 – 4.0 Buffer / pH20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2 / 8.0
Experimental temperature10.0 Facility / beamlineSealy Center For Structural Biology, UTMB-G / Rigaku BioSAXS-1000
Wavelength0.15418 nmExposure10.0 s × 3

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Neurexin 1a L5L6
View sequence
ADPVTFKTKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGANLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGGVAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSASTSHHHHHH
protein—Q9ULB1904–1307monomerMolecule count 144.499 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1470[3]0.016548–0.68539 1/AHas uncertainty columnMissing 031sasbdb/entries/95/sasdd95/source/SASDD95.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.00.5Å
i0Guinier39647.648.5arbitrary
i0P(r)39110.089.0arbitrary
mwExperimental39.8—kDa
mwGuinier I(0)39.60.1kDa
mwPorod43.0—kDa
porod_volumePorod69.0—ų
rgGuinier3.020.01Å
rgP(r)3.00.01Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2Buffer concentration—
pH8.0Additives—
Buffer descriptionN1a Buffer
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-09-06Storage / measurement temperature10.0 / 10.0
Exposure time10.0Frames3
Wavelength0.15418Sample-to-detector distance0.4838
SourceX-ray in houseDetectorPilatus 100K
Facility / beamlineSealy Center For Structural Biology, UTMB-G / Rigaku BioSAXS-1000 · Galveston, TX, USA
q range0.165 – 6.854Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:167 field values
Field pathRaw value
codeSASDD95
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleStructural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.
project.publication.titleStructural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.
project.publication.author_listLiu J, Misra A, Reddy MVVVS, White MA, Ren G, Rudenko G
project.publication.journalJ Mol Biol
project.publication.doi10.1016/j.jmb.2018.08.026
project.publication.pmid30193986
project.publication.published_date2018 Oct 19
project.statusreleased
project.submitted_date2018-03-22
project.released_date2018-09-10
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 100K
experiment.instrument.detector.resolution172.0
experiment.instrument.nameSealy Center For Structural Biology, UTMB-G
experiment.instrument.cityGalveston, TX
experiment.instrument.countryUSA
experiment.instrument.beamline_nameRigaku BioSAXS-1000
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNeurexin 1a L5L6
experiment.sample.molecule[0].short_nameL5L6
experiment.sample.molecule[0].sequenceADPVTFKTKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLH YVFDLGNGANLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLK SDLYIGGVAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERG CEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWPPNDRP STRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESNA IINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQGQPFQG QLSGLYYNGLKVLNMAAENDANIAIVGNVRLVG EVPSASTSHHHHHH
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeQ9ULB1
experiment.sample.molecule[0].uniprot_range_first904
experiment.sample.molecule[0].uniprot_range_last1307
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGTALLQRGGCFLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPKWNACCESEMSFQLKT RSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPATLLADTPVNDGAWHSVRIR RQFRNTTLFIDQVEAKWVEVKSKRRDMTVFSGLFVGGLPPELRAAALKLTLASVREREPF KGWIRDVRVNSSQVLPVDSGEVKLDDEPPNSGGGSPCEAGEEGEGGVCLNGGVCSVVDDQ AVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKGKEEYIATFKGSEYFCYDLSQNP IQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNG KFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSP STADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQGDPKMKIHGVVAFKCENVATLD PITFETPESFISLPKWNAKKTGSISFDFRTTEPNGLILFSHGKPRHQKDAKHPQMIKVDF FAIEMLDGHLYLLLDMGSGTIKIKALLKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTA PGESEILDLDDELYLGGLPENKAGLVFPTEVWTALLNYGYVGCIRDLFIDGQSKDIRQMA EVQSTAGVKPSCSKETAKPCLSNPCKNNGMCRDGWNRYVCDCSGTGYLGRSCEREATVLS YDGSMFMKIQLPVVMHTEAEDVSLRFRSQRAYGILMATTSRDSADTLRLELDAGRVKLTV NLDCIRINCNSSKGPETLFAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHT RLEFHNIETGIITERRYLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFR NIIADPVTFKTKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKG YLHYVFDLGNGANLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNL DLKSDLYIGGVAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGC EGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWP PNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAI EESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQG QPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSSMTTESTATAMQSEMSTS IMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEPSSGGLANPTR AGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRN YISNSAQSNGAVVKEKQPSSAKSSNKNKKNKDKEYYV
experiment.sample.molecule[0].mw44.499
experiment.sample.molecule[0].total_mw44.499
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentN1a Buffer
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNeurexin 1a L5L6
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressTexas, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of Texas Medical Branch
experiment.contributor[0].affiliation[0].webpagehttp://www.bmb.utmb.edu/
experiment.contributor[0].contributor_nameMark
experiment.contributor[0].contributor_surnameWhite
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-09-06
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time10.0
experiment.number_of_frames3
experiment.wavelength0.15418
experiment.sample_detector_distance0.4838
experiment.concentration_min2.0
experiment.concentration_max4.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.165
experiment.s_max6.854
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
fits[0].models[0].symmetryP1
fits[0].models[0].commentnull
fits[0].models[0].user85
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fits[0].softwareCORAL
fits[0].chi_square_value1.3
fits[0].p_value0.000154
fits[0].fit_residual_plotSASDD95_fit1_fitresiduals_img.png
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fits[0].descriptionnull
fits[1].models[]
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fits[1].softwareEOM/GAJOE
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fits[1].p_value0.0
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fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSAXS data from solutions of Neurexin 1a L5L6 in 20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2, pH 8 were collected on a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) using a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 4 mg/ml were measured at 10°C. Three successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unitarbitrary
experimental_mw39.8
experimental_mw_errornull
guinier_i0_mw39.6
guinier_i0_mw_error0.1
porod_mw43.0
porod_mw_errornull
pddf_i039110.0
pddf_i0_error89.0
guinier_i039647.6
guinier_i0_error48.5
pddf_rg3.0
pddf_rg_error0.01
guinier_rg3.02
guinier_rg_error0.01
pddf_dmax10.0
pddf_dmax_error0.5
porod_volume69.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last17
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDD95_datloglog_img.png
symmetrynull
last_modified2021-08-18T17:28:22.895829+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDD95
statussuccess
started_at2026-08-11T14:42:21.747905+00:00
finished_at2026-08-11T14:42:30.368786+00:00
source_last_modified2021-08-18T17:28:22.895829+02:00
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View the complete summary.json source
{
  "code": "SASDD95",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.",
    "publication": {
      "title": "Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.",
      "author_list": "Liu J, Misra A, Reddy MVVVS, White MA, Ren G, Rudenko G",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2018.08.026",
      "pmid": "30193986",
      "published_date": "2018 Oct 19"
    },
    "status": "released",
    "submitted_date": "2018-03-22",
    "released_date": "2018-09-10"
  },
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View the complete manifest.json source
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