SASDDA6

Class I chitinase 2 from Agave tequilana

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Class I chitinase 2 from Agave tequilana

Concentration0.46 – 7.5 Buffer / pHMES 50 mM / 6.0
Experimental temperature15.0 Facility / beamlineStanford Synchrotron Radiation Lightsource (SSRL) / BL4-2
Wavelength0.1127 nmExposure1.0 s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Chitinase 2
View sequence
>CHIAT2A.TEQUILANASNAQQCGSQAGGAVCPNGLCCSQFGYCGSTSPYCGNGCQSQCGGGSSPTPNPPSGGGGGGGGGGSGVGSIISSSLFDQMLLHRNDAACPANGFYTYDAFVAAANAFSGFATTGDADTQKREIAAFLAQTSHETTGGWPTAPDGPYSWGYCFLQEQGNPGDYCVPNDQWPCAPGKKYYGRGPIQISYNYNYGPCGNAIRSDLLNNPDLVASDPTVSFKTALWFWMTPQSPKPSCHDVITRAWTPSAADQAAGRVPGFGVITNIINGGVECGHGSDSRDEDRVGFYKRYCDILGVSFGDNLDCGNQSHF
proteinAgave tequilana——–—monomerMolecule count 131.9 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1597[3]0.00666787–0.322595 1/AHas uncertainty columnMissing 00sasbdb/entries/a6/sasdda6/source/SASDDA6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.75—Å
i0Guinier107.280.13—
i0P(r)108.4——
mwExperimental31.9—kDa
porod_volumePorod49.0—ų
rgGuinier2.380.047Å
rgP(r)2.51—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer nameMES 50 mMBuffer concentration—
pH6.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2017-04-19Storage / measurement temperature4.0 / 15.0
Exposure time1.0Frames—
Wavelength0.1127Sample-to-detector distance1.8
SourceX-ray synchrotronDetectorRayonix MX225-HE
Facility / beamlineStanford Synchrotron Radiation Lightsource (SSRL) / BL4-2 · Menlo Park, CA, USA
q range0.067 – 3.226Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDDA6
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleA biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
project.publication.titleA biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
project.publication.author_listSierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A
project.publication.journalFEBS J
project.publication.doi10.1111/febs.14993
project.publication.pmid31291689
project.publication.published_date2019 Jul 10
project.statusreleased
project.submitted_date2018-05-28
project.released_date2019-07-16
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experiment.instrument.detector.resolutionnull
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experiment.instrument.cityMenlo Park, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBL4-2
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experiment.sample.molecule[0].long_nameChitinase 2
experiment.sample.molecule[0].short_nameChiAt2
experiment.sample.molecule[0].sequence>ChiAt2 A.tequilana SNAQQCGSQAGGAVCPNGLCCSQFGYCGSTSPYCGNGCQSQCGGGSSPTPNPPSGGGGGG GGGGSGVGSIISSSLFDQMLLHRNDAACPANGFYTYDAFVAAANAFSGFATTGDADTQKR EIAAFLAQTSHETTGGWPTAPDGPYSWGYCFLQEQGNPGDYCVPNDQWPCAPGKKYYGRG PIQISYNYNYGPCGNAIRSDLLNNPDLVASDPTVSFKTALWFWMTPQSPKPSCHDVITRA WTPSAADQAAGRVPGFGVITNIINGGVECGHGSDSRDEDRVGFYKRYCDILGVSFGDNLD CGNQSHF
experiment.sample.molecule[0].organismAgave tequilana
experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw31.9
experiment.sample.molecule[0].total_mw31.9
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experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.nameMES 50 mM
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
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experiment.sample.buffer.pkanull
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experiment.sample.purity_methodnull
experiment.sample.nameClass I chitinase 2 from Agave tequilana
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMexico
experiment.contributor[0].affiliation[0].full_nameIQ, UNAM
experiment.contributor[0].affiliation[0].webpagehttps://www.iquimica.unam.mx/
experiment.contributor[0].contributor_nameYusvel
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experiment.storage_temperature4.0
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experiment_descriptionSynchrotron SAXS data from solutions of Chitinase 2 from Agave tequilana in MES 50 mM, pH 6, were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 7.5 mg/ml were measured at 15°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
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last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDDA6
statussuccess
started_at2026-08-11T16:31:33.238111+00:00
finished_at2026-08-11T16:31:41.074681+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
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  "status": "Published",
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  "angular_unit": "1/A",
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    "title": "A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.",
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      "author_list": "Sierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A",
      "journal": "FEBS J",
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View the complete manifest.json source
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