SASDDA7

Synthetic FRET biosensor Twitch 2B Ca-free

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2021-12-20T11:10:57.864084+01:00

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Synthetic FRET biosensor Twitch 2B Ca-free

Concentration	— – 5.0	Buffer / pH	20 mM MOPS 100 mM NaCl 10 mM EDTA / 7.0
Experimental temperature	20.0	Facility / beamline	ESRF / BM29
Wavelength	0.0992 nm	Exposure	1.0 s × 12

Molecular Components

Component	Type / organism	UniProt and Construct	Oligomeric State	Molecular weight
Synthetic FRET biosensor Twitch 2B View sequence MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAARMQVADASEEELSECFRIFDFDGNGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMVENVQPIYPELMGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIED	proteinsynthetic construct	——–—	monomerMolecule count 1	62.399 kDa

Experimental Curves

Curve	Points / columns	q range	Uncertainty quality	Negative-intensity points	Source file
1	1043[3]	0.03369227–4.975169 1/nm	Has uncertainty columnMissing 0	3	sasbdb/entries/a7/sasdda7/source/SASDDA7.dat

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2. Measurements Reported by SASBDB Reported Results

Metric	Method	Value	Uncertainty	Unit
i0	Guinier	44.38	—	arbitrary
mw	Experimental	52.05	—	kDa
rg	Guinier	4.11	—	nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

Category	Path	Download status	Size
curve	sasbdb/entries/a7/sasdda7/source/SASDDA7.dat	downloaded	57959
full_entry_zip	sasbdb/entries/a7/sasdda7/source/SASDDA7.zip	downloaded	17855
pddf	sasbdb/entries/a7/sasdda7/source/SASDDA7.out	not_listed	—
sascif	sasbdb/entries/a7/sasdda7/source/SASDDA7.sascif	downloaded	61474
summary	sasbdb/entries/a7/sasdda7/source/summary.json	downloaded	5914

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name	20 mM MOPS 100 mM NaCl 10 mM EDTA	Buffer concentration	—
pH	7.0	Additives	—
Buffer description	Buffer B
Purity measurement method	—	Extinction coefficient	—
Absorbance	—	Scattering contrast	—
Specific volume / dry volume	— / —	Mixture / deuteration	— / —

Acquisition Conditions and Instrument

Measurement date	2015-03-17	Storage / measurement temperature	20.0 / 20.0
Exposure time	1.0	Frames	12
Wavelength	0.0992	Sample-to-detector distance	2.864
Source	X-ray synchrotron	Detector	Pilatus 1M
Facility / beamline	ESRF / BM29 · Grenoble, France
q range	0.034 – 4.975	Sample volume / flow rate	— / —

Original SASBDB Plots

Downloadable Files

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pddf	sasbdb/entries/a7/sasdda7/source/SASDDA7.out	not_listed	—	—	DownloadView source file
sascif	sasbdb/entries/a7/sasdda7/source/SASDDA7.sascif	downloaded	61474	38b339f61255d31f954488bd542bdaf68683c835f1ea0dcc5ed99e23f6881e38	DownloadView source fileSource site
summary	sasbdb/entries/a7/sasdda7/source/summary.json	downloaded	5914	e7d415fb75ff030ca4b33787763ff912adc0106dec04872c3daea19e063890d7	DownloadView source fileSource site

curve：source record

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full_entry_zip：source record and ZIP contents (1 items)

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pddf：source record

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sascif：source record

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summary：source record

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：129 field values

Field path	Raw value
code	SASDDA7
status	Published
type_of_curve	Single concentration
angular_unit	1/nm
project.title	Synthetic FRET biosensor Twitch 2B
project.publication	null
project.status	released
project.submitted_date	2018-05-25
project.released_date	2021-12-20
pddf_data	null
intensities_data	https://www.sasbdb.org/media/intensities_files/SASDDA7.dat
intensities_log_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA7_dat_img.png
intensities_kratky_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA7_kratky_img.png
pddf_plot	null
intensities_guinier_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA7_guinier_img.png
sascif_data	https://www.sasbdb.org/media/sascif/sascif_files/SASDDA7.sascif
experiment.instrument.detector.type	Dectris
experiment.instrument.detector.name	Pilatus 1M
experiment.instrument.detector.resolution	172.0
experiment.instrument.name	ESRF
experiment.instrument.city	Grenoble
experiment.instrument.country	France
experiment.instrument.beamline_name	BM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_source	X-ray synchrotron
experiment.instrument.point_source	null
experiment.instrument.line_collimation	null
experiment.instrument.sample_path_length	null
experiment.instrument.line_collimation_slitlength	null
experiment.instrument.line_collimation_integrationwidth	null
experiment.instrument.xray_energy	null
experiment.instrument.beam_profile_ah	null
experiment.instrument.beam_profile_al	null
experiment.sample.molecule[0].long_name	Synthetic FRET biosensor Twitch 2B
experiment.sample.molecule[0].short_name	TW2B
experiment.sample.molecule[0].sequence	MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT TGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGN VYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSTQSKLSKDPNEKRDHMVLLEFVTAARMQVADASEEELSECFRIFDFD GNGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMV ENVQPIYPELMGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPN EKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVE LDGDVNGHKFSVRGEGEGDATNGKLTLKLICTTGKLPVPWPTLVTTLGYG LMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKI RHNIED
experiment.sample.molecule[0].organism	synthetic construct
experiment.sample.molecule[0].uniprot_code	null
experiment.sample.molecule[0].uniprot_range_first	null
experiment.sample.molecule[0].uniprot_range_last	null
experiment.sample.molecule[0].oligomerization	monomer
experiment.sample.molecule[0].molecular_type	protein
experiment.sample.molecule[0].uniprot_sequence	null
experiment.sample.molecule[0].mw	62.399
experiment.sample.molecule[0].total_mw	62.399
experiment.sample.molecule[0].number_molecules	1
experiment.sample.molecule[0].complex_state	False
experiment.sample.molecule[0].deuteration	null
experiment.sample.molecule[0].molecule_source	biological
experiment.sample.molecule[0].molecule_description	null
experiment.sample.buffer.name	20 mM MOPS 100 mM NaCl 10 mM EDTA
experiment.sample.buffer.concentration_unit	null
experiment.sample.buffer.comment	Buffer B
experiment.sample.buffer.additive	null
experiment.sample.buffer.concentration	null
experiment.sample.buffer.pka	null
experiment.sample.buffer.ph	7.0
experiment.sample.buffer.deuteration	null
experiment.sample.purity_method	null
experiment.sample.name	Synthetic FRET biosensor Twitch 2B Ca-free
experiment.sample.ext_coefficient	null
experiment.sample.contrast	null
experiment.sample.specific_vol	null
experiment.sample.dry_vol	null
experiment.sample.absorbption	null
experiment.sample.deuteration	null
experiment.sample.mixture	null
experiment.contributor[0].affiliation[0].short_name	null
experiment.contributor[0].affiliation[0].address	Germany
experiment.contributor[0].affiliation[0].full_name	Max Planck Institute for Biophysical Chemistry
experiment.contributor[0].affiliation[0].webpage	https://www.mpibpc.mpg.de
experiment.contributor[0].contributor_name	Pablo
experiment.contributor[0].contributor_surname	Trigo Mourino
experiment.contributor[0].orcid	null
experiment.concentration_method	null
experiment.concentration_unit	null
experiment.date	2015-03-17
experiment.storage_temperature	20.0
experiment.cell_temperature	20.0
experiment.exposure_time	1.0
experiment.number_of_frames	12
experiment.wavelength	0.0992
experiment.sample_detector_distance	2.864
experiment.concentration_min	null
experiment.concentration_max	5.0
experiment.sample_volume	null
experiment.flow_rate	null
experiment.s_min	0.034
experiment.s_max	4.975
experiment.total_exposure_time	null
experiment.seccolumn	null
fits	[]
estimated_volume_method	null
pddf_software	null
pddf_software_version	null
i0_calibration_standard	null
description	null
experiment_description	Synchrotron SAXS data from solutions of the synthetic FRET biosensor, Twitch 2B, in the absence of calcium, in 20 mM MOPS 100 mM NaCl 10 mM EDTA, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 12 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags	[]
intensity_unit	arbitrary
experimental_mw	52.05
experimental_mw_error	null
guinier_i0_mw	null
guinier_i0_mw_error	null
porod_mw	null
porod_mw_error	null
pddf_i0	null
pddf_i0_error	null
guinier_i0	44.38
guinier_i0_error	null
pddf_rg	null
pddf_rg_error	null
guinier_rg	4.11
guinier_rg_error	null
pddf_dmax	null
pddf_dmax_error	null
porod_volume	null
porod_volume_error	null
estimated_volume	null
estimated_volume_error	null
guinier_point_first	7
guinier_point_last	32
pddf_point_first	null
pddf_point_last	null
i0_calibration_standard_data	null
intensities_log_log_plot	SASDDA7_datloglog_img.png
symmetry	null
last_modified	2021-12-20T11:10:57.864084+01:00
bragg_peak	[]

manifest.json：33 field values

Field path	Raw value
code	SASDDA7
status	success
started_at	2026-08-11T15:25:11.786893+00:00
finished_at	2026-08-11T15:25:18.695733+00:00
source_last_modified	2021-12-20T11:10:57.864084+01:00
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files[0].size	5914
files[0].sha256	e7d415fb75ff030ca4b33787763ff912adc0106dec04872c3daea19e063890d7
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files[1].size	57959
files[1].sha256	dd16ac908267464251c5c5cb7ae52cf225f50d95b72a0a83e202ee45e3ac8a32
files[1].role	curve
files[2].role	pddf
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View the complete summary.json source

{
  "code": "SASDDA7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Synthetic FRET biosensor Twitch 2B",
    "publication": null,
    "status": "released",
    "submitted_date": "2018-05-25",
    "released_date": "2021-12-20"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDA7.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA7_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA7_kratky_img.png",
  "pddf_plot": null,
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  "experiment": {
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        "name": "Pilatus 1M",
        "resolution": 172.0
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      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Synthetic FRET biosensor Twitch 2B",
          "short_name": "TW2B",
          "sequence": "MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT\r\nTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF\r\nFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGN\r\nVYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNH\r\nYLSTQSKLSKDPNEKRDHMVLLEFVTAARMQVADASEEELSECFRIFDFD\r\nGNGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMV\r\nENVQPIYPELMGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPN\r\nEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVE\r\nLDGDVNGHKFSVRGEGEGDATNGKLTLKLICTTGKLPVPWPTLVTTLGYG\r\nLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD\r\nTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKI\r\nRHNIED",
          "organism": "synthetic construct",
          "uniprot_code": null,
          "uniprot_range_first": null,
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          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
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        }
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        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
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      "ext_coefficient": null,
      "contrast": null,
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    },
    "contributor": [
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            "short_name": null,
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            "full_name": "Max Planck Institute for Biophysical Chemistry",
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        "orcid": null
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    "concentration_method": null,
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    "concentration_min": null,
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  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 52.05,
  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
  "porod_mw": null,
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  "pddf_i0": null,
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  "guinier_i0": 44.38,
  "guinier_i0_error": null,
  "pddf_rg": null,
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  "guinier_rg": 4.11,
  "guinier_rg_error": null,
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  "guinier_point_first": 7,
  "guinier_point_last": 32,
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  "symmetry": null,
  "last_modified": "2021-12-20T11:10:57.864084+01:00",
  "bragg_peak": []
}

View the complete manifest.json source

{
  "code": "SASDDA7",
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