SASDDA9

Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M KBr

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

Individual (top) and spatially averaged (DAMFILT, bottom) ab initio models are displayed.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M KBr

浓度— – 3.1 缓冲液 / pH50 mM HEPES, 2 M KBr, 0.02% NaN3 / 7.0
实验温度25.0 设备 / 束线ESRF / BM29
波长0.09919 nm曝光2.0 s × 10

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
VNG0258H/RosR
查看序列
>VNG0258H/ROSRMSEAQPDARSDARDLTAFQKNILTVLGEEARYGLAIKRELEEYYGEEVNHGRLYPNLDDLVNKGLVEKSELDKRTNEYALTNEGFDAVVDDLEWTLSKFVADADRRERVETIVADDAAALEHHHHHH
proteinHalobacterium salinarum NRC-1Q9HSF4—–—dimer分子数 214.533 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.03369227–4.975169 1/nm含误差列缺失 06sasbdb/entries/a9/sasdda9/source/SASDDA9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.7—nm
i0Guinier5.870.033—
i0P(r)5.6870.05—
mwExperimental24.4—kDa
mwPorod29.0—kDa
porod_volumePorod46.0—nm³
rgGuinier2.330.11nm
rgP(r)2.230.05nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES, 2 M KBr, 0.02% NaN3缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-03-07储存 / 测量温度25.0 / 25.0
曝光时间2.0帧数10
波长0.09919样品-探测器距离2.864
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.034 – 4.975样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

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project.publication.titleThe 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.
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project.publication.doi10.1016/j.jsb.2018.08.008
project.publication.pmid30110657
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project.statusreleased
project.submitted_date2018-06-29
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descriptionIndividual (top) and spatially averaged (DAMFILT, bottom) ab initio models are displayed.
experiment_descriptionSynchrotron SAXS data from solutions Halobacterium salinarum VNG0258H/RosR in 50 mM HEPES, 2 M KBr, 0.02% NaN3, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.10 mg/ml was measured at 25°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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statussuccess
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查看完整 summary.json 原文
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  "status": "Published",
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    "title": "The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.",
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      "title": "The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.",
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      "doi": "10.1016/j.jsb.2018.08.008",
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}
查看完整 manifest.json 原文
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