SASDDB8

Periplasmic domain of inner membrane protein GspL, dimer

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Periplasmic domain of inner membrane protein GspL, dimer

浓度2.0 – 13.7 缓冲液 / pH50 mM TRIS, 100 mM NaCl / 7.5
实验温度15.0 设备 / 束线SOLEIL / SWING
波长0.1033 nm曝光— s × 19

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Type II secretion system protein L, periplasmic domain
查看序列
WSHPQFEKGGGDRYAAQSAELYRQLFPEDRKLINLRAQFDQHLADSASSGGEGQLLGLLGQAATVIGGEPTVSVEQLDFSAARGDVALQVRAPGFDVLERLRSRLSESGLAVQLGSASRDGSTVSARLVIGG
proteinPseudomonas aeruginosaP25060261–382dimer分子数 213.966 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11086[4]0.00795134474354–0.621731749979 1/A含误差列缺失 04sasbdb/entries/b8/sasddb8/source/SASDDB8.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.5—Å
i0Guinier0.03913392.5e-05arbitrary
i0P(r)0.03865—arbitrary
mwExperimental22.8—kDa
mwGuinier I(0)36.4—kDa
rgGuinier2.2430.07Å
rgP(r)2.21—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM TRIS, 100 mM NaCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明pH=7.5 at 25°C
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-04-08储存 / 测量温度4.0 / 15.0
曝光时间—帧数19
波长0.1033样品-探测器距离—
光源X-ray synchrotron探测器AVIEX PCCD170170
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q 范围0.08 – 6.139样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(9 项)
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pddf:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:221 个字段值
字段路径原始值
codeSASDDB8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
project.publication.titleStructure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
project.publication.author_listFulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN
project.publication.journalSci Rep
project.publication.doi10.1038/s41598-018-34956-w
project.publication.pmid30425318
project.publication.published_date2018 Nov 13
project.statusreleased
project.submitted_date2018-06-11
project.released_date2018-11-13
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameType II secretion system protein L, periplasmic domain
experiment.sample.molecule[0].short_nameGspLperi
experiment.sample.molecule[0].sequenceWSHPQFEKGG GDRYAAQSAE LYRQLFPEDR KLINLRAQFD QHLADSASSG GEGQLLGLLG QAATVIGGEP TVSVEQLDFS AARGDVALQV RAPGFDVLER LRSRLSESGL AVQLGSASRD GSTVSARLVI GG
experiment.sample.molecule[0].organismPseudomonas aeruginosa
experiment.sample.molecule[0].uniprot_codeP25060
experiment.sample.molecule[0].uniprot_range_first261
experiment.sample.molecule[0].uniprot_range_last382
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].total_mw27.932
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
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experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
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experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
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experiment.sample.purity_methodnull
experiment.sample.namePeriplasmic domain of inner membrane protein GspL, dimer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
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experiment.contributor[0].affiliation[0].full_nameGhent University
experiment.contributor[0].affiliation[0].webpagehttps://www.ugent.be/
experiment.contributor[0].contributor_nameAleksandra
experiment.contributor[0].contributor_surnameFulara
experiment.contributor[0].orcid
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tags[]
intensity_unitarbitrary
experimental_mw22.8
experimental_mw_errornull
guinier_i0_mw36.4
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porod_mwnull
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last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDB8
statussuccess
started_at2026-08-11T15:49:35.592361+00:00
finished_at2026-08-11T15:49:45.213751+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDDB8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.",
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      "title": "Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.",
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查看完整 manifest.json 原文
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