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SASDDC8

Periplasmic domain of inner membrane protein GspL, tetramer

数据类型：SASBDB 实验数据 状态：Published 曲线类型：SEC-SAS 最后更新：2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Periplasmic domain of inner membrane protein GspL, tetramer

浓度— – — 缓冲液 / pH50 mM TRIS, 100 mM NaCl / 7.5
实验温度15.0 设备 / 束线SOLEIL / SWING
波长0.1033 nm曝光— s × 15

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Type II secretion system protein L, periplasmic domain
查看序列
WSHPQFEKGGGDRYAAQSAELYRQLFPEDRKLINLRAQFDQHLADSASSGGEGQLLGLLGQAATVIGGEPTVSVEQLDFSAARGDVALQVRAPGFDVLERLRSRLSESGLAVQLGSASRDGSTVSARLVIGG
proteinPseudomonas aeruginosaP25060261–382dimer分子数 213.966 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11100[4]0.0–0.621731749979 1/A含误差列缺失 017sasbdb/entries/c8/sasddc8/source/SASDDC8.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.5—Å
i0Guinier0.03796222.3e-05arbitrary
i0P(r)0.03726—arbitrary
mwExperimental56.0—kDa
mwGuinier I(0)57.0—kDa
rgGuinier3.1750.37Å
rgP(r)3.098—Å

这些数值是 SASBDB 来源记录，不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”；未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM TRIS, 100 mM NaCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明pH=7.5 at 25°C
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-04-08储存 / 测量温度— / 15.0
曝光时间—帧数15
波长0.1033样品-探测器距离—
光源X-ray synchrotron探测器AVIEX PCCD170170
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q 范围0.068 – 6.139样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/c8/sasddc8/source/SASDDC8.outdownloaded38075d485250eb891f2e9802610ca66b62a6e96f71d77907e6537e4beda65a7116012下载查看原文件源站
sascifsasbdb/entries/c8/sasddc8/source/SASDDC8.sascifdownloaded90208c85c7af8d8d99de94daecc4fce54a403ac7bee19e82ac07e17a3d1bf667653dc下载查看原文件源站
summarysasbdb/entries/c8/sasddc8/source/summary.jsondownloaded58268cbc8a19cc74c8b0ac3c68dbde8fe391dbe24075c826aaa521f8e95df64c78ae下载查看原文件源站
curve：来源记录
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full_entry_zip：来源记录与 ZIP 内部目录（2 项）
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pddf：来源记录
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sascif：来源记录
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summary：来源记录
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全部来源字段（无筛选）

这里自动展开来源记录中的每一个字段，包括空值、列表成员和页面上方已展示过的字段。

summary.json：134 个字段值
字段路径原始值
codeSASDDC8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
project.publication.titleStructure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
project.publication.author_listFulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN
project.publication.journalSci Rep
project.publication.doi10.1038/s41598-018-34956-w
project.publication.pmid30425318
project.publication.published_date2018 Nov 13
project.statusreleased
project.submitted_date2018-06-11
project.released_date2018-11-13
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDDC8.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDDC8.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDC8_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDC8_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDC8_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDC8_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDDC8.sascif
experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameType II secretion system protein L, periplasmic domain
experiment.sample.molecule[0].short_nameGspLperi
experiment.sample.molecule[0].sequenceWSHPQFEKGG GDRYAAQSAE LYRQLFPEDR KLINLRAQFD QHLADSASSG GEGQLLGLLG QAATVIGGEP TVSVEQLDFS AARGDVALQV RAPGFDVLER LRSRLSESGL AVQLGSASRD GSTVSARLVI GG
experiment.sample.molecule[0].organismPseudomonas aeruginosa
experiment.sample.molecule[0].uniprot_codeP25060
experiment.sample.molecule[0].uniprot_range_first261
experiment.sample.molecule[0].uniprot_range_last382
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSGVSALFLPPASTAGADGELAVWWVQDGECRRAPFAQALAEIRAPWRLYLPVEAVTACA VNLPTQKARWLRQSLPFAVEEQLADDVEQMHLALGPALADGRHRVFAVQRTWLAAWLALA EGAGKAPASLHVDADCLPGEGSCLFWLEERWLLGGSGAVRLACGSEDWPVLRDSCPPPQR AFAAQEVAPLEGVEVQALAGNPHVWLSEQPLGTDLAQAEFAARQQSSQWRRWRPLLGLVG LWLVLQWGFTLVQAWQLQREGDRYAAQSAELYRQLFPEDRKLINLRAQFDQHLADSASSG GEGQLLGLLGQAATVIGGEPTVSVEQLDFSAARGDVALQVRAPGFDVLERLRSRLSESGL AVQLGSASRDGSTVSARLVIGG
experiment.sample.molecule[0].mw13.966
experiment.sample.molecule[0].total_mw27.932
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM TRIS, 100 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentpH=7.5 at 25°C
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePeriplasmic domain of inner membrane protein GspL, tetramer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressGent, Belgium
experiment.contributor[0].affiliation[0].full_nameGhent University
experiment.contributor[0].affiliation[0].webpagehttps://www.ugent.be/
experiment.contributor[0].contributor_nameAleksandra
experiment.contributor[0].contributor_surnameFulara
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-04-08
experiment.storage_temperaturenull
experiment.cell_temperature15.0
experiment.exposure_timenull
experiment.number_of_frames15
experiment.wavelength0.1033
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.068
experiment.s_max6.139
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.6
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw56.0
experimental_mw_errornull
guinier_i0_mw57.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.03726
pddf_i0_errornull
guinier_i00.0379622
guinier_i0_error2.3e-05
pddf_rg3.098
pddf_rg_errornull
guinier_rg3.175
guinier_rg_error0.37
pddf_dmax10.5
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDC8_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json：36 个字段值
字段路径原始值
codeSASDDC8
statussuccess
started_at2026-08-11T15:50:14.891561+00:00
finished_at2026-08-11T15:50:23.297720+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
files[0].statusdownloaded
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查看完整 summary.json 原文
{
  "code": "SASDDC8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.",
    "publication": {
      "title": "Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.",
      "author_list": "Fulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN",
      "journal": "Sci Rep",
      "doi": "10.1038/s41598-018-34956-w",
      "pmid": "30425318",
      "published_date": "2018 Nov 13"
    },
    "status": "released",
    "submitted_date": "2018-06-11",
    "released_date": "2018-11-13"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDC8.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDC8_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDC8_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDC8_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDC8.sascif",
  "experiment": {
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        "name": "AVIEX PCCD170170",
        "resolution": null
      },
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      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
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          "long_name": "Type II secretion system protein L, periplasmic domain",
          "short_name": "GspLperi",
          "sequence": "WSHPQFEKGG GDRYAAQSAE LYRQLFPEDR KLINLRAQFD\r\nQHLADSASSG GEGQLLGLLG QAATVIGGEP TVSVEQLDFS\r\nAARGDVALQV RAPGFDVLER LRSRLSESGL AVQLGSASRD\r\nGSTVSARLVI GG",
          "organism": "Pseudomonas aeruginosa",
          "uniprot_code": "P25060",
          "uniprot_range_first": 261,
          "uniprot_range_last": 382,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Periplasmic domain of inner membrane protein GspL, tetramer",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
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    },
    "contributor": [
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            "short_name": null,
            "address": "Gent, Belgium",
            "full_name": "Ghent University",
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          }
        ],
        "contributor_name": "Aleksandra",
        "contributor_surname": "Fulara",
        "orcid": ""
      }
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    "date": "2016-04-08",
    "storage_temperature": null,
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  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 56.0,
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  "guinier_i0_mw": 57.0,
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  "porod_mw": null,
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  "pddf_i0": 0.03726,
  "pddf_i0_error": null,
  "guinier_i0": 0.0379622,
  "guinier_i0_error": 2.3e-05,
  "pddf_rg": 3.098,
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  "intensities_log_log_plot": "SASDDC8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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