SASDDE5

NBD-MsbA (apo)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · NBD-MsbA (apo)

浓度— – 18.0 缓冲液 / pH30 mM Tris, 150 mM NaCl, 0.5 mM TCEP / 7.5
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA
查看序列
RATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQIEEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLLEHRGVYAQLHKMQFGQ
proteinEscherichia coliP60752—–—monomer分子数 127.263 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11186[3]0.0611364–3.3196 1/nm含误差列缺失 00sasbdb/entries/e5/sasdde5/source/SASDDE5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.30.5nm
i0Guinier0.0220.0011/cm
i0P(r)0.0220.0011/cm
mwExperimental30.125.0kDa
mwPorod29.629.6kDa
porod_volumePorod47.395.0nm³
rgGuinier2.150.1nm
rgP(r)2.170.1nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称30 mM Tris, 150 mM NaCl, 0.5 mM TCEP缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度2.842
比体积 / 干体积0.738 / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-05-30储存 / 测量温度20.0 / 20.0
曝光时间0.05帧数20
波长0.124样品-探测器距离3.3
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.061 – 3.32样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDDE5
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleConformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs.
project.publication.titleConformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs.
project.publication.author_listJosts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
project.publication.journalStructure
project.publication.doi10.1016/j.str.2018.05.007
project.publication.pmid29937358
project.publication.published_date2018 Aug 7
project.statusreleased
project.submitted_datenull
project.released_date2018-06-27
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA
experiment.sample.molecule[0].short_nameNBD-MsbA
experiment.sample.molecule[0].sequenceRATGDVEFRNVTFT YPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGEILMDGHDLREYTLASLRNQVA LVSQNVHLFNDTVANNIAYARTEQYSREQIEEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIA IARALLRDSPILILDEATSALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERG THNDLLEHRGVYAQLHKMQFGQ
experiment.sample.molecule[0].organismEscherichia coli
experiment.sample.molecule[0].uniprot_codeP60752
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFMLSLLKPLLDDGFGKTD RSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRRRLFGHMMGMPVSFFDKQS TGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMMFYYSWQLSIILIVLAPIVSI AIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVLIFGGQEVETKRFDKVSNRMRLQ GMKMVSASSISDPIIQLIASLALAFVLYAASFPSVMDSLTAGTITVVFSSMIALMRPLKS LTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRVIERATGDVEFRNVTFTYPGRDVPALR NINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGEILMDGHDLREYTLASLRNQVA LVSQNVHLFNDTVANNIAYARTEQYSREQIEEAARMAYAMDFINKMDNGLDTVIGENGVL LSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQAALDELQKNRTSLVIAHRLS TIEKADEIVVVEDGVIVERGTHNDLLEHRGVYAQLHKMQFGQ
experiment.sample.molecule[0].mw27.263
experiment.sample.molecule[0].total_mw27.263
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name30 mM Tris, 150 mM NaCl, 0.5 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNBD-MsbA (apo)
experiment.sample.ext_coefficientnull
experiment.sample.contrast2.842
experiment.sample.specific_vol0.738
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodA280
experiment.concentration_unitnull
experiment.date2017-05-30
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance3.3
experiment.concentration_minnull
experiment.concentration_max18.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.061
experiment.s_max3.32
experiment.total_exposure_time1.0
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version2.8.3
i0_calibration_standardwater
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw30.12
experimental_mw_error5.0
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw29.6
porod_mw_error29.6
pddf_i00.022
pddf_i0_error0.001
guinier_i00.022
guinier_i0_error0.001
pddf_rg2.17
pddf_rg_error0.1
guinier_rg2.15
guinier_rg_error0.1
pddf_dmax7.3
pddf_dmax_error0.5
porod_volume47.39
porod_volume_error5.0
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last198
pddf_point_first12
pddf_point_last1186
i0_calibration_standard_datanull
intensities_log_log_plotSASDDE5_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDE5
statussuccess
started_at2026-08-11T14:46:15.692570+00:00
finished_at2026-08-11T14:46:24.240971+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDDE5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs.",
    "publication": {
      "title": "Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs.",
      "author_list": "Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H",
      "journal": "Structure",
      "doi": "10.1016/j.str.2018.05.007",
      "pmid": "29937358",
      "published_date": "2018 Aug 7"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2018-06-27"
  },
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        "resolution": null
      },
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      "country": "Germany",
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          "mw": 27.263,
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          "deuteration": null,
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        "deuteration": null
      },
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}
查看完整 manifest.json 原文
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