SASDDG5

Mammalian prion protein mRNA (PrP mRNA wild type)

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Mammalian prion protein mRNA (PrP mRNA wild type)

Concentration0.5 – 4.0 Buffer / pH10 mM Tris buffer / 7.5
Experimental temperature20.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
octo-repeat PrP mRNA
View sequence
AACACUGGGGGCAGCCGAUACCCGGGGCAGGGCAGCCCUGGAGGCAACCGCUACCCACCUCAGGGCGGUGGUGGCUGGGGGCAGCCUCAUGGUGGUGGCUGGGGGCAGCCUCAUGGUGGUGGCUGGGGGCAGCCCCAUGGUGGUGGCUGGGGACAGCCUCAUGGUGGUGGCUGGGGUCAAGGAGGUGGCACCCACCUCAGGGCGGUGGUGGCUGGGGGCC
RNAhuman PrP ORF——–—dimerMolecule count 271.89 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11761[3]0.119365–3.78783 1/nmHas uncertainty columnMissing 00sasbdb/entries/g5/sasddg5/source/SASDDG5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)25.01.0nm
i0Guinier24740.0775.0arbitrary
i0P(r)24050.0500.0arbitrary
mwExperimental130.0—kDa
mwGuinier I(0)130.020.0kDa
mwPorod150.0—kDa
porod_volumePorod165.0—nm³
rgGuinier7.350.28nm
rgP(r)7.350.05nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10 mM Tris bufferBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-11-04Storage / measurement temperature20.0 / 20.0
Exposure time0.05Frames20
Wavelength0.124Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.119 – 3.788Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDDG5
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleOcta-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.
project.publication.titleOcta-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.
project.publication.author_listCzech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
project.publication.journalSci Rep
project.publication.doi10.1038/s41598-019-39213-2
project.publication.pmid30792490
project.publication.published_date2019 Feb 21
project.statusreleased
project.submitted_date2018-03-29
project.released_date2019-02-25
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameocto-repeat PrP mRNA
experiment.sample.molecule[0].short_namePrPmRNA
experiment.sample.molecule[0].sequenceAACACUGGGGGCAGCCGAUACCCGGGGCAGGGCAGCCCUGGAGGCAACCGCUACCCACCUCAGGGCGGUGGUGGCUGGGGGCAGCCUCAUGGUGGUGGCUGGGGGCAGCCUCAUGGUGGUGGCUGGGGGCAGCCCCAUGGUGGUGGCUGGGGACAGCCUCAUGGUGGUGGCUGGGGUCAAGGAGGUGGCACCCAC CUCAGGGCGGUGGUGGCUGGGGGCC
experiment.sample.molecule[0].organismhuman PrP ORF
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw71.89
experiment.sample.molecule[0].total_mw143.78
experiment.sample.molecule[0].number_molecules2
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experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10 mM Tris buffer
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
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experiment.sample.nameMammalian prion protein mRNA (PrP mRNA wild type)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_namePetr
experiment.contributor[0].contributor_surnameKonarev
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-11-04
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.05
experiment.number_of_frames20
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experiment_descriptionSynchrotron SAXS data from solutions of mammalian prion protein mRNA (PrP mRNA wild type) in 10 mM Tris buffer, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 4 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
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experimental_mw_errornull
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porod_mw150.0
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guinier_i024740.0
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symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDDG5
statussuccess
started_at2026-08-11T14:47:36.428078+00:00
finished_at2026-08-11T14:47:46.798705+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
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  "status": "Published",
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    "title": "Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.",
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      "title": "Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.",
      "author_list": "Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z",
      "journal": "Sci Rep",
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      "pmid": "30792490",
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View the complete manifest.json source
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