SASDDG8

Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-06-21T07:56:39.492710+02:00

Synchrotron SAXS data from solutions of a fragment of the cytoplasmic region of the human integrin Beta4 subunit. This fragment includes the final part of a region named the "connecting segment" the third and fourth fibronectin type III domains (FnIII-3,4). The protein contains the point mutation R1463A. Samples at different solute concentration in the range 0.9 – 7.1 mg/ml were obtained by 2-fold serial dilution and were measured.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A

Concentration0.9 – 7.1 Buffer / pH20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT / 7.5
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.05 s × 30

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Integrin beta-4 (1436-1666) R1463A
View sequence
GSHMLPRDYSTLTSVSSHDSRLTAGVPDTPTALVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIES
proteinHomo sapiensP161441436–1666monomerMolecule count 125.52 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11671[3]0.0564852–4.45043 1/nmHas uncertainty columnMissing 0130sasbdb/entries/g8/sasddg8/source/SASDDG8.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.5—nm
i0Guinier0.0164—1/cm
i0P(r)0.01637—1/cm
mwExperimental22.71—kDa
mwPorod24.1—kDa
porod_volumePorod36.1—nm³
rgGuinier2.417—nm
rgP(r)2.444—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTTBuffer concentration20.0 mM
pH7.5Additives150 mM NaCl, 5% glycerol, 3 mM DTT
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-11-26Storage / measurement temperature— / 10.0
Exposure time0.05Frames30
Wavelength0.124Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.056 – 4.45Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:167 field values
Field pathRaw value
codeSASDDG8
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleIntegrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes.
project.publication.titleIntegrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes.
project.publication.author_listManso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
project.publication.journalStructure
project.publication.doi10.1016/j.str.2019.03.016
project.publication.pmid31006587
project.publication.published_date2019 Jun 4
project.statusreleased
project.submitted_date2018-06-07
project.released_date2019-03-14
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDDG8.dat
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIntegrin beta-4 (1436-1666) R1463A
experiment.sample.molecule[0].short_nameB4 CS-FnIII-3,4
experiment.sample.molecule[0].sequenceGSHM LPRDY STLTSVSSHDSRLTAGVPDTPTALVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGG ELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCP LPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAF RVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIES
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP16144
experiment.sample.molecule[0].uniprot_range_first1436
experiment.sample.molecule[0].uniprot_range_last1666
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMAGPRPSPWARLLLAALISVSLSGTLANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRR CNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFEL EVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSV PQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAIL QTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYR TQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSNIVELLE EAFNRIRSNLDIRALDSPRGLRTEVTSKMFQKTRTGSFHIRRGEVGIYQVQLRALEHVDG THVCQLPEDQKGNIHLKPSFSDGLKMDAGIICDVCTCELQKEVRSARCSFNGDFVCGQCV CSEGWSGQTCNCSTGSLSDIQPCLREGEDKPCSGRGECQCGHCVCYGEGRYEGQFCEYDN FQCPRTSGFLCNDRGRCSMGQCVCEPGWTGPSCDCPLSNATCIDSNGGICNGRGHCECGR CHCHQQSLYTDTICEINYSAIHPGLCEDLRSCVQCQAWGTGEKKGRTCEECNFKVKMVDE LKRAEEVVVRCSFRDEDDDCTYSYTMEGDGAPGPNSTVLVHKKKDCPPGSFWWLIPLLLL LLPLLALLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLR SGNLKGRDVVRWKVTNNMQRPGFATHAASINPTELVPYGLSLRLARLCTENLLKPDTREC AQLRQEVEENLNEVYRQISGVHKLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKPALLK LTEKQVEQRAFHDLKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQL LVEAIDVPAGTATLGRRLVNITIIKEQARDVVSFEQPEFSVSRGDQVARIPVIRRVLDGG KSQVSYRTQDGTAQGNRDYIPVEGELLFQPGEAWKELQVKLLELQEVDSLLRGRQVRRFH VQLSNPKFGAHLGQPHSTTIIIRDPDELDRSFTSQMLSSQPPPHGDLGAPQNPNAKAAGS RKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYG AQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLV NDDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQP KRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQRPSVSDDTGCGWKFEPLLG EELDLRRVTWRLPPELIPRLSASSGRSSDAEAPHGPPDDGGAGGKGGSLPRSATPGPPGE HLVNGRMDFAFPGSTNSLHRMTTTSAAAYGTHLSPHVPHRVLSTSSTLTRDYNSLTRSEH SHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGY SVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIES QVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEM AQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGG PFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEF VSRTLTTSGTLSTHMDQQFFQT
experiment.sample.molecule[0].mw25.52
experiment.sample.molecule[0].total_mw25.52
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl, 5% glycerol, 3 mM DTT
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameIntegrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameUSAL
experiment.contributor[0].affiliation[0].addressSalamanca, Spain
experiment.contributor[0].affiliation[0].full_nameLa Universidad de Salamanca
experiment.contributor[0].affiliation[0].webpagehttp://www.usal.es
experiment.contributor[0].contributor_nameJose M
experiment.contributor[0].contributor_surnamede Pereda
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-11-26
experiment.storage_temperaturenull
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames30
experiment.wavelength0.124
experiment.sample_detector_distance3.1
experiment.concentration_min0.9
experiment.concentration_max7.1
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.056
experiment.s_max4.45
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius1.5
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fits[0].p_value0.665832
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fits[1].softwareEOM/GAJOE
fits[1].chi_square_value1.032
fits[1].p_value0.665832
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fits[1].descriptionnull
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSynchrotron SAXS data from solutions of a fragment of the cytoplasmic region of the human integrin Beta4 subunit. This fragment includes the final part of a region named the "connecting segment" the third and fourth fibronectin type III domains (FnIII-3,4). The protein contains the point mutation R1463A. Samples at different solute concentration in the range 0.9 – 7.1 mg/ml were obtained by 2-fold serial dilution and were measured.
experiment_descriptionSynchrotron SAXS data from solutions of the integrin beta4 fragment (point mutant R1463A) in 20 mM sodium phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 7.1 mg/ml were measured at 10°C. 30 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unit1/cm
experimental_mw22.71
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw24.1
porod_mw_errornull
pddf_i00.01637
pddf_i0_errornull
guinier_i00.0164
guinier_i0_errornull
pddf_rg2.444
pddf_rg_errornull
guinier_rg2.417
guinier_rg_errornull
pddf_dmax9.5
pddf_dmax_errornull
porod_volume36.1
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last184
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDG8_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDDG8
statussuccess
started_at2026-08-11T15:52:54.147763+00:00
finished_at2026-08-11T15:53:03.090250+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDDG8",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes.",
    "publication": {
      "title": "Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes.",
      "author_list": "Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM",
      "journal": "Structure",
      "doi": "10.1016/j.str.2019.03.016",
      "pmid": "31006587",
      "published_date": "2019 Jun 4"
    },
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    "submitted_date": "2018-06-07",
    "released_date": "2019-03-14"
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      "ext_coefficient": null,
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          {
            "short_name": "USAL",
            "address": "Salamanca, Spain",
            "full_name": "La Universidad de Salamanca",
            "webpage": "http://www.usal.es"
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    "concentration_unit": null,
    "date": "2013-11-26",
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  "last_modified": "2023-06-21T07:56:39.492710+02:00",
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}
View the complete manifest.json source
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