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{
"code": "SASDDG9",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/nm",
"project": {
"title": "OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.",
"publication": {
"title": "OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.",
"author_list": "Sluchanko NN, Slonimskiy YB, Shirshin EA, Moldenhauer M, Friedrich T, Maksimov EG",
"journal": "Nat Commun",
"doi": "10.1038/s41467-018-06195-0",
"pmid": "30250028",
"published_date": "2018 Sep 24"
},
"status": "released",
"submitted_date": "2018-07-04",
"released_date": "2018-08-07"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDG9.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDG9.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDG9_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDG9_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDG9_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDG9_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDG9.sascif",
"experiment": {
"instrument": {
"detector": {
"type": null,
"name": "Pilatus 2M",
"resolution": null
},
"name": "PETRA III",
"city": "DESY; Hamburg",
"country": "Germany",
"beamline_name": "EMBL P12",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Fluorescence recovery protein",
"short_name": null,
"sequence": ">FRPcc\r\nMRGSHHHHHHTDPATMLQTAEAPWSQAETQSAHALFRKAYQRELDGLCATVQAQASQCTQIDDLWKLHDFLSAKRHEIDGKYDDRQSVIIFVFAQLLKEGLVQAEELTFLAADKQSKIKALARL",
"organism": "Synechocystis sp. PCC 6803",
"uniprot_code": "P74103",
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MLQTAEAPWSQAETQSAHALFRKAYQRELDGLLATVQAQASQITQIDDLWKLHDFLSAKR\nHEIDGKYDDRQSVIIFVFAQLLKEGLVQAEELTFLAADKQSKIKALARL",
"mw": 14.09,
"total_mw": 28.18,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Orange carotenoid-binding protein",
"short_name": "OCP",
"sequence": ">dNTE\r\nMRGSHHHHHHTDPATVPATIARFSQLNAEDQLALIWFAYLEMGKTLTIAAPGAASMQLAENALKEIQAMGPLQQTQAMCDLANRADTPLCRTYASWSPNIKLGFWYRLGELMEQG FVAPIPAGYQLSANANAVLATIQGLESGQQITVLRNAVVDMGFTAGKDGKRIAEPVVPPQ DTASRTKVSIEGVTNATVLNYMDNLNANDFDTLIELFTSDGALQPPFQRPIVGKENVLRF FREECQNLKLIPERGVTEPAEDGFTQIKVTGKVQTPWFGGNVGMNIAWRFLLNPEGKIFF VAIDLLASPKELLNFAR",
"organism": "Synechocystis sp. PCC 6803",
"uniprot_code": "P74102",
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MPFTIDSARGIFPNTLAADVVPATIARFSQLNAEDQLALIWFAYLEMGKTLTIAAPGAAS\nMQLAENALKEIQAMGPLQQTQAMCDLANRADTPLCRTYASWSPNIKLGFWYRLGELMEQG\nFVAPIPAGYQLSANANAVLATIQGLESGQQITVLRNAVVDMGFTAGKDGKRIAEPVVPPQ\nDTASRTKVSIEGVTNATVLNYMDNLNANDFDTLIELFTSDGALQPPFQRPIVGKENVLRF\nFREECQNLKLIPERGVTEPAEDGFTQIKVTGKVQTPWFGGNVGMNIAWRFLLNPEGKIFF\nVAIDLLASPKELLNFAR",
"mw": 34.28,
"total_mw": 34.28,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "20 mM Tris, 150 mM NaCl, 3% glycerol",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.6,
"deuteration": null
},
"purity_method": null,
"name": "The 2:1 complex of Synechocystis disulphide-trapped Fluorescence recovery protein dimer (CC mutant) and orange carotenoid-binding protein-ΔNTE (orange form)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Moscow, Russia",
"full_name": "A.N. Bach Institute of Biochemistry",
"webpage": "http://www.fbras.ru/en/"
}
],
"contributor_name": "Nikolai",
"contributor_surname": "Sluchanko",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2017-09-01",
"storage_temperature": 20.0,
"cell_temperature": 10.0,
"exposure_time": 0.05,
"number_of_frames": 20,
"wavelength": 0.124,
"sample_detector_distance": null,
"concentration_min": null,
"concentration_max": 2.41,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.11,
"s_max": 5.064,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDG9_fit1_model1_img.png",
"software": "GASBOR",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDG9_fit1_model1.pdb",
"model_mw": null,
"bead_radius": 1.9,
"log": null,
"symmetry": "none",
"comment": null,
"user": 267
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDG9_fit1_fit_img.png",
"software": "GASBOR",
"chi_square_value": 1.06,
"p_value": 0.590997,
"fit_residual_plot": "SASDDG9_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDG9_fit1.fir",
"fit_log": null,
"software_version": null,
"description": null
},
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDG9_fit2_model1_img.png",
"software": "GASBOR",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDG9_fit2_model1.pdb",
"model_mw": null,
"bead_radius": 1.9,
"log": null,
"symmetry": "none",
"comment": null,
"user": 267
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDG9_fit2_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 1.023,
"p_value": 0.580508,
"fit_residual_plot": "SASDDG9_fit2_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDG9_fit2.fit",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/gasbor101.log",
"software_version": null,
"description": null
},
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDG9_fit3_model1_img.png",
"software": "CORAL",
"pdb_link": [],
"model_title": null,
"type_of_model": "mix",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDG9_fit3_model1.pdb",
"model_mw": 62.17,
"bead_radius": 1.9,
"log": "https://www.sasbdb.org/media/log_files/rcrl1.log",
"symmetry": null,
"comment": null,
"user": 267
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDG9_fit3_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 1.018,
"p_value": 0.102317,
"fit_residual_plot": "SASDDG9_fit3_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDG9_fit3.fit",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/rcrl1700.log",
"software_version": null,
"description": null
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of the 2:1 complex of the disulfide trapped FRP dimer (CC) and OCP-deltaNTE mutant of the Synechocystis OCP (orange form) in 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 3 % v/v glycerol, pH 7.6 were collected at the EMBL-P12 bioSAXS beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.41 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 63.9,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 63.9,
"porod_mw_error": null,
"pddf_i0": 0.03241,
"pddf_i0_error": 0.0001,
"guinier_i0": 0.03215,
"guinier_i0_error": 7e-05,
"pddf_rg": 3.15,
"pddf_rg_error": 0.02,
"guinier_rg": 3.03,
"guinier_rg_error": 0.01,
"pddf_dmax": 13.0,
"pddf_dmax_error": null,
"porod_volume": 102.2,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 2,
"guinier_point_last": 116,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDDG9_datloglog_img.png",
"symmetry": null,
"last_modified": "2023-11-13T09:35:03.156077+01:00",
"bragg_peak": []
}
