SASDDJ2

Calcium-bound polcalcin Phl p 7

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-05-18T10:06:15.993142+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Calcium-bound polcalcin Phl p 7

Concentration5.0 – 15.0 Buffer / pH0.15M NaCl, 0.025M Hepes, pH 7.4, 100 uM Ca2+ / 7.4
Experimental temperature10.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.1127 nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
polcalcin Phl p 7
View sequence
ADDMERIFKRFDTNGDGKISLSELTDALRTLGSTSADEVQRMMAEIDTDGDGFIDFNEFISFCNANPGLMKDVAKVF
proteinPhleum pratenseO82040—–—monomerMolecule count 18.545 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1502[3]0.020978–0.325586 1/AHas uncertainty columnMissing 00sasbdb/entries/j2/sasddj2/source/SASDDJ2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)3.62—Å
i0Guinier52.540.075arbitrary
i0P(r)52.19—arbitrary
mwExperimental8.545—kDa
mwPorod8.5—kDa
porod_volumePorod13.6—ų
rgGuinier1.290.003Å
rgP(r)1.27—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name0.15M NaCl, 0.025M Hepes, pH 7.4, 100 uM Ca2+Buffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2011-12-08Storage / measurement temperature— / 10.0
Exposure time—Frames—
Wavelength0.1127Sample-to-detector distance—
SourceX-ray synchrotronDetectorMAR 165 CCD
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.21 – 3.256Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDDJ2
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleSolution structures of polcalcin Phl p 7 in three ligation states: Apo-, hemi-Mg2+-bound, and fully Ca2+-bound.
project.publication.titleSolution structures of polcalcin Phl p 7 in three ligation states: Apo-, hemi-Mg2+-bound, and fully Ca2+-bound.
project.publication.author_listHenzl MT, Sirianni AG, Wycoff WG, Tan A, Tanner JJ
project.publication.journalProteins
project.publication.doi10.1002/prot.24186
project.publication.pmid23011803
project.publication.published_date2013 Feb
project.statusreleased
project.submitted_date2018-01-09
project.released_date2018-01-10
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experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
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experiment.sample.molecule[0].long_namepolcalcin Phl p 7
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceADDMERIFKRFDTNGDGKISLSELTDALRTLGSTSADEVQRMMAEIDTDGDGFIDFNEFISFCNANPGLMKDVAKVF
experiment.sample.molecule[0].organismPhleum pratense
experiment.sample.molecule[0].uniprot_codeO82040
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experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameMizzou
experiment.contributor[0].affiliation[0].addressColumbia, MO, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of Missouri-Columbia
experiment.contributor[0].affiliation[0].webpagehttp://missouri.edu/
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experiment_descriptionSynchrotron SAXS data from solutions of calcium-bound polcalcin Phl p 7 in 0.15 M NaCl, 0.025 M HEPES, 100 uM Ca2+, pH 7.4, were collected on the 12.3.1 SIBYLS beam line at the Advanced Light Source storage ring (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 5 and 15 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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View the complete summary.json source
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}
View the complete manifest.json source
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