SASDDK4

p-hydroxyphenylacetate 3-hydroxylase, reductase component (mutant): 4 mg/ml of Y207A C1 in the absence of p-hydroxyphenylacetic acid (HPA)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · p-hydroxyphenylacetate 3-hydroxylase, reductase component (mutant): 4 mg/ml of Y207A C1 in the absence of p-hydroxyphenylacetic acid (HPA)

浓度— – 4.0 缓冲液 / pH50 mM MOPS, 0.5 mM EDTA, 1 mM DTT, and 5% glycerol / 7.0
实验温度16.0 设备 / 束线Synchrotron Light Research Institute (SLRI) / BL1.3W
波长0.13776 nm曝光600.0 s × 1

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
p-hydroxyphenylacetate 3-hydroxylase (HPAH), reductase component Y207A mutant
查看序列
MNQLNTAIVEKEVIDPMAFRRALGNFATGVTIMTAQTSSGERVGVTANSFNSVSLDPALVLWSIDKKSSSYRIFEEATHFGVNILSAAQIELSNRFARRSEDKFANIEFDLGVGNIPLFKNCSAAFECERYNIVEGGDHWIIIGRVVKFHDHGRSPLLYHQGAYSAVLPHPSLNMKSETAEGVFPGRLYDNMYYLLTQAVRAYQNDAQPKQLASGFRTSEARLLLVLESKTASSKCDLQREVAMPIREIEEATKILSEKGLLIDNGQHYELTEQGNACAHMLYKIAESHQEEVFAKYTVDERKLFKNMLKDLIGI
proteinAcinetobacter baumannii——–—dimer分子数 235.32 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1913[3]0.0299702264–0.278066881 1/A含误差列缺失 043sasbdb/entries/k4/sasddk4/source/SASDDK4.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)7.1—Å
i0Guinier144.050.48arbitrary
i0P(r)149.2—arbitrary
mwExperimental72.0—kDa
mwPorod69.0—kDa
porod_volumePorod111.0—ų
rgGuinier2.4630.165Å
rgP(r)2.52—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM MOPS, 0.5 mM EDTA, 1 mM DTT, and 5% glycerol缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-03-07储存 / 测量温度4.0 / 16.0
曝光时间600.0帧数1
波长0.13776样品-探测器距离1.3323
光源X-ray synchrotron探测器Rayonix SX165
机构 / 束线Synchrotron Light Research Institute (SLRI) / BL1.3W · Nakhon Ratchasima, Thailand
q 范围0.3 – 2.781样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDDK4
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleCrystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands.
project.publication.titleCrystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands.
project.publication.author_listYuenyao A, Petchyam N, Kamonsutthipaijit N, Chaiyen P, Pakotiprapha D
project.publication.journalArch Biochem Biophys
project.publication.doi10.1016/j.abb.2018.06.010
project.publication.pmid29940152
project.publication.published_date2018 Sep 1
project.statusreleased
project.submitted_date2018-02-28
project.released_date2018-06-27
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experiment.instrument.detector.resolution15.0
experiment.instrument.nameSynchrotron Light Research Institute (SLRI)
experiment.instrument.cityNakhon Ratchasima
experiment.instrument.countryThailand
experiment.instrument.beamline_nameBL1.3W
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namep-hydroxyphenylacetate 3-hydroxylase (HPAH), reductase component Y207A mutant
experiment.sample.molecule[0].short_nameY207A C1 reductase
experiment.sample.molecule[0].sequenceMNQLNTAIVE KEVIDPMAFR RALGNFATGV TIMTAQTSSG ERVGVTANSF NSVSLDPALV LWSIDKKSSS YRIFEEATHF GVNILSAAQI ELSNRFARRS EDKFANIEFD LGVGNIPLFK NCSAAFECER YNIVEGGDHW IIIGRVVKFH DHGRSPLLYH QGAYSAVLPH PSLNMKSETA EGVFPGRLYD NMYYLLTQAV RAYQNDAQPK QLASGFRTSE ARLLLVLESK TASSKCDLQR EVAMPIREIE EATKILSEKG LLIDNGQHYE LTEQGNACAH MLYKIAESHQ EEVFAKYTVD ERKLFKNMLK DLIGI
experiment.sample.molecule[0].organismAcinetobacter baumannii
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw35.32
experiment.sample.molecule[0].total_mw70.64
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM MOPS, 0.5 mM EDTA, 1 mM DTT, and 5% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namep-hydroxyphenylacetate 3-hydroxylase, reductase component (mutant): 4 mg/ml of Y207A C1 in the absence of p-hydroxyphenylacetic acid (HPA)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
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experiment.sample.dry_volnull
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experiment.contributor[0].affiliation[0].short_namenull
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experiment.contributor[0].affiliation[0].full_nameMahidol University
experiment.contributor[0].affiliation[0].webpagehttps://mahidol.ac.th/
experiment.contributor[0].contributor_nameAnan
experiment.contributor[0].contributor_surnameYuenyao
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-03-07
experiment.storage_temperature4.0
experiment.cell_temperature16.0
experiment.exposure_time600.0
experiment.number_of_frames1
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experiment.flow_ratenull
experiment.s_min0.3
experiment.s_max2.781
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw72.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw69.0
porod_mw_errornull
pddf_i0149.2
pddf_i0_errornull
guinier_i0144.05
guinier_i0_error0.48
pddf_rg2.52
pddf_rg_errornull
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guinier_rg_error0.165
pddf_dmax7.1
pddf_dmax_errornull
porod_volume111.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first23
guinier_point_last83
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDK4_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDK4
statussuccess
started_at2026-08-11T14:25:08.907256+00:00
finished_at2026-08-11T14:25:17.456637+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Crystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands.",
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      "title": "Crystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands.",
      "author_list": "Yuenyao A, Petchyam N, Kamonsutthipaijit N, Chaiyen P, Pakotiprapha D",
      "journal": "Arch Biochem Biophys",
      "doi": "10.1016/j.abb.2018.06.010",
      "pmid": "29940152",
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    },
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDDK4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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