Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea

{
  "code": "SASDDM3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.",
    "publication": {
      "title": "Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.",
      "author_list": "Franke D, Jeffries CM, Svergun DI",
      "journal": "Biophys J",
      "doi": "10.1016/j.bpj.2018.04.018",
      "pmid": "29874600",
      "published_date": "2018 Jun 5"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2018-06-07"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDM3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDM3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDM3_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM3_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDM3.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Ribonuclease pancreatic",
          "short_name": "RNase",
          "sequence": ">sp|P61823|RNAS1_BOVIN Ribonuclease pancreatic OS=Bos taurus GN=RNASE1 PE=1 SV=1\nMALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRN\nLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPN\nCAYKTTQANKHIIVACEGNPYVPVHFDASV\n\n",
          "organism": "Bos taurus",
          "uniprot_code": "P61823",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRN\nLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPN\nCAYKTTQANKHIIVACEGNPYVPVHFDASV",
          "mw": 16.46,
          "total_mw": 16.46,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "10 mM HCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 1.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
        ],
        "contributor_name": "Cy M",
        "contributor_surname": "Jeffries",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2013-07-29",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.03,
    "number_of_frames": 17,
    "wavelength": 0.124,
    "sample_detector_distance": 3.1,
    "concentration_min": null,
    "concentration_max": 5.97,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.054,
    "s_max": 3.495,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "4.6",
  "i0_calibration_standard": null,
  "description": "Included in the additional files of the full-entry zip archive are SAXS data measured from folded RNAse (rnase.dat - also refer to SASBDB entry SASDDL3) as well as unfolded carboyxamidomethylated ribonuclease A (rnaseU.dat) and unfolded RNAse in the presence of 1 M urea (rnaseUU1.dat) or 2 M urea (rnaseUU2.dat).",
  "experiment_description": "Synchrotron SAXS data from solutions of carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea in 10 mM HCl, pH 1, were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.97 mg/ml was measured at 10°C. 17 successive 0.030 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 13.4,
  "experimental_mw_error": null,
  "guinier_i0_mw": 13.4,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 498.2,
  "pddf_i0_error": null,
  "guinier_i0": 484.7,
  "guinier_i0_error": 5.04,
  "pddf_rg": 2.55,
  "pddf_rg_error": null,
  "guinier_rg": 2.31,
  "guinier_rg_error": 0.03,
  "pddf_dmax": 9.0,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 154,
  "guinier_point_last": 254,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDDM3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T14:03:20.026568+00:00",
  "finished_at": "2026-08-11T14:03:28.362786+00:00",
  "source_last_modified": "2023-06-21T07:56:39.492710+02:00",
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