SASDDM6

Calbindin-D28K

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Calbindin-D28K

浓度— – 12.2 缓冲液 / pH20 mM Tris, 150mM NaCl / 7.8
实验温度25.0 设备 / 束线Diamond Light Source / B21
波长0.1 nm曝光3.0 s × 14

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Calbindin
查看序列
GPHMAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN
proteinHomo sapiensP05937—–—monomer分子数 130.316 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11661[3]0.0037–0.36955921 1/A含误差列缺失 00sasbdb/entries/m6/sasddm6/source/SASDDM6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.95—Å
i0Guinier0.085630.0001arbitrary
i0P(r)0.08571—arbitrary
mwExperimental25.0—kDa
mwPorod29.0—kDa
porod_volumePorod46.0—ų
rgGuinier2.1420.005Å
rgP(r)2.14—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris, 150mM NaCl缓冲液浓度—
pH7.8添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2018-02-28储存 / 测量温度4.0 / 25.0
曝光时间3.0帧数14
波长0.1样品-探测器距离4.0
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.037 – 3.696样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDDM6
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleThe X-ray structure of human calbindin-D28K: an improved model.
project.publication.titleThe X-ray structure of human calbindin-D28K: an improved model.
project.publication.author_listNoble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR
project.publication.journalActa Crystallogr D Struct Biol
project.publication.doi10.1107/S2059798318011610
project.publication.pmid30289411
project.publication.published_date2018 Oct 1
project.statusreleased
project.submitted_date2018-05-17
project.released_date2018-10-04
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experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometry1 x 5 mm
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameCalbindin
experiment.sample.molecule[0].short_nameCALB1
experiment.sample.molecule[0].sequenceGPHMAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP05937
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw30.316
experiment.sample.molecule[0].total_mw30.316
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris, 150mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCalbindin-D28K
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressBrighton, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Sussex
experiment.contributor[0].affiliation[0].webpagehttp://www.sussex.ac.uk/
experiment.contributor[0].contributor_nameJames
experiment.contributor[0].contributor_surnameNoble
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-02-28
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time3.0
experiment.number_of_frames14
experiment.wavelength0.1
experiment.sample_detector_distance4.0
experiment.concentration_minnull
experiment.concentration_max12.2
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.037
experiment.s_max3.696
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIF
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fits[0].models[0].bead_radius2.5
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fits[0].chi_square_value1.156
fits[0].p_value0.172169
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.5a
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of calbindin-D28K in 20 mM Tris, 150mM NaCl, pH 7.8, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 12.20 mg/ml was measured at 25°C. 14 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw25.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw29.0
porod_mw_errornull
pddf_i00.08571
pddf_i0_errornull
guinier_i00.08563
guinier_i0_error0.0001
pddf_rg2.14
pddf_rg_errornull
guinier_rg2.142
guinier_rg_error0.005
pddf_dmax6.95
pddf_dmax_errornull
porod_volume46.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first38
guinier_point_last259
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDM6_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDM6
statussuccess
started_at2026-08-11T15:12:26.890568+00:00
finished_at2026-08-11T15:12:35.102850+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDDM6",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "The X-ray structure of human calbindin-D28K: an improved model.",
    "publication": {
      "title": "The X-ray structure of human calbindin-D28K: an improved model.",
      "author_list": "Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR",
      "journal": "Acta Crystallogr D Struct Biol",
      "doi": "10.1107/S2059798318011610",
      "pmid": "30289411",
      "published_date": "2018 Oct 1"
    },
    "status": "released",
    "submitted_date": "2018-05-17",
    "released_date": "2018-10-04"
  },
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          "long_name": "Calbindin",
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          "uniprot_code": "P05937",
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          "oligomerization": "monomer",
          "molecular_type": "protein",
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      },
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      "name": "Calbindin-D28K",
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查看完整 manifest.json 原文
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