SASDDQ6

The ferredoxin protease, FusC

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2020-04-22T08:28:46.473948+02:00

Additional SEC parameters: Column type: GE Healthcare Superdex S200 10/300; Flow rate: 0.4 ml/min; Sample injection concentration: 10 mg/ml; Injection volume: 100 µl.

▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · The ferredoxin protease, FusC

Concentration	— – 10.0	Buffer / pH	20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol / 7.8
Experimental temperature	20.0	Facility / beamline	Australian Synchrotron / SAXS/WAXS
Wavelength	0.103 nm	Exposure	— s × —

Molecular Components

Component	Type / organism	UniProt and Construct	Oligomeric State	Molecular weight
Ferredoxin Protease View sequence EEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDIRLIVDVGSIDEKDNESGVAHMVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYERTMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERKIILEEWRGKLGVAERMNQQRVQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREIQRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRHRLLTQITMSAVTRQVRRQKAELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISAVLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETREFADWVQQLTIVWQQDRPYVGSQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQWALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGKKVYLTATSQAGFMATAMNPWQAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEADQLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMSLARQKANDDQSVGGKRASEMTKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLATIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIASKYLKTSLRDDALGIYRMRVDSELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTKITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYNDPRYLSNASKLADSITLESVRAMSAKLYNPDNRVLYITLPQEVKE	proteinPectobacterium atrosepticum SCRI1043	Q6D8U326–924	monomerMolecule count 1	101.253 kDa

Experimental Curves

Curve	Points / columns	q range	Uncertainty quality	Negative-intensity points	Source file
1	700[3]	0.0064398–0.279033 1/A	Has uncertainty columnMissing 0	8	sasbdb/entries/q6/sasddq6/source/SASDDQ6.dat

▼

2. Measurements Reported by SASBDB Reported Results

Metric	Method	Value	Uncertainty	Unit
dmax	P(r)	12.84	—	Å
i0	Guinier	0.13	0.00011	arbitrary
i0	P(r)	0.1299	—	arbitrary
mw	Experimental	100.0	—	kDa
mw	Porod	89.2	—	kDa
porod_volume	Porod	151.6	—	Å³
rg	Guinier	3.68	0.013	Å
rg	P(r)	3.74	—	Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

▶

4. Source File Index Source Files

Category	Path	Download status	Size
curve	sasbdb/entries/q6/sasddq6/source/SASDDQ6.dat	downloaded	32262
full_entry_zip	sasbdb/entries/q6/sasddq6/source/SASDDQ6.zip	downloaded	23083
pddf	sasbdb/entries/q6/sasddq6/source/SASDDQ6.out	downloaded	52406
sascif	sasbdb/entries/q6/sasddq6/source/SASDDQ6.sascif	downloaded	75687
summary	sasbdb/entries/q6/sasddq6/source/summary.json	downloaded	8268

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name	20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol	Buffer concentration	—
pH	7.8	Additives	—
Buffer description	—
Purity measurement method	—	Extinction coefficient	—
Absorbance	—	Scattering contrast	—
Specific volume / dry volume	— / —	Mixture / deuteration	— / —

Acquisition Conditions and Instrument

Measurement date	2017-04-06	Storage / measurement temperature	20.0 / 20.0
Exposure time	—	Frames	—
Wavelength	0.103	Sample-to-detector distance	2.68
Source	X-ray synchrotron	Detector	Pilatus 1M
Facility / beamline	Australian Synchrotron / SAXS/WAXS · Melbourne, Australia
q range	0.064 – 2.79	Sample volume / flow rate	— / —

Original SASBDB Plots

Downloadable Files

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full_entry_zip	sasbdb/entries/q6/sasddq6/source/SASDDQ6.zip	downloaded	23083	47232061dd5565aa21da4976e517a36c831382cb635d2a40a7985c54a1496727	DownloadView source fileSource site
pddf	sasbdb/entries/q6/sasddq6/source/SASDDQ6.out	downloaded	52406	fd8cefb087a911ec042ec67f9044205451ee316e543a586345ba85a0e1bd46ea	DownloadView source fileSource site
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summary	sasbdb/entries/q6/sasddq6/source/summary.json	downloaded	8268	fedac11f0da0cb1d8e0b2f979146b530adff6c48f0b98fcbf5560996143dd125	DownloadView source fileSource site

curve：source record

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full_entry_zip：source record and ZIP contents (2 items)

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pddf：source record

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sascif：source record

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summary：source record

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：134 field values

Field path	Raw value
code	SASDDQ6
status	Published
type_of_curve	SEC-SAS
angular_unit	1/A
project.title	FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.
project.publication.title	FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.
project.publication.author_list	Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
project.publication.journal	PLoS Biol
project.publication.doi	10.1371/journal.pbio.2006026
project.publication.pmid	30071011
project.publication.published_date	2018 Aug
project.status	released
project.submitted_date	2018-05-19
project.released_date	2018-06-13
pddf_data	https://www.sasbdb.org/media/p_of_R_files/SASDDQ6.out
intensities_data	https://www.sasbdb.org/media/intensities_files/SASDDQ6.dat
intensities_log_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_dat_img.png
intensities_kratky_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_kratky_img.png
pddf_plot	https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDQ6_pofr_img.png
intensities_guinier_plot	https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_guinier_img.png
sascif_data	https://www.sasbdb.org/media/sascif/sascif_files/SASDDQ6.sascif
experiment.instrument.detector.type	Dectris
experiment.instrument.detector.name	Pilatus 1M
experiment.instrument.detector.resolution	172.0
experiment.instrument.name	Australian Synchrotron
experiment.instrument.city	Melbourne
experiment.instrument.country	Australia
experiment.instrument.beamline_name	SAXS/WAXS
experiment.instrument.beam_geometry	Point
experiment.instrument.type_of_source	X-ray synchrotron
experiment.instrument.point_source	True
experiment.instrument.line_collimation	null
experiment.instrument.sample_path_length	null
experiment.instrument.line_collimation_slitlength	null
experiment.instrument.line_collimation_integrationwidth	null
experiment.instrument.xray_energy	null
experiment.instrument.beam_profile_ah	null
experiment.instrument.beam_profile_al	null
experiment.sample.molecule[0].long_name	Ferredoxin Protease
experiment.sample.molecule[0].short_name	FusC
experiment.sample.molecule[0].sequence	EEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDIRLIVDVGSIDEKDNESGVAH MVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYERTMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERK IILEEWRGKLGVAERMNQQRVQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREI QRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRHRLLTQITMSAVTRQVRRQKA ELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISAVLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETRE FADWVQQLTIVWQQDRPYVGSQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQW ALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGKKVYLTATSQAGFMATAMNPW QAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEADQLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMS LARQKANDDQSVGGKRASEMTKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLA TIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIASKYLKTSLRDDALGIYRMRVD SELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTKITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYND PRYLSNASKLADSITLESVRAMSAKLYNPDNRVLYITLPQEVKE
experiment.sample.molecule[0].organism	Pectobacterium atrosepticum SCRI1043
experiment.sample.molecule[0].uniprot_code	Q6D8U3
experiment.sample.molecule[0].uniprot_range_first	26
experiment.sample.molecule[0].uniprot_range_last	924
experiment.sample.molecule[0].oligomerization	monomer
experiment.sample.molecule[0].molecular_type	protein
experiment.sample.molecule[0].uniprot_sequence	MNWKKCCWLSLTAVGLLAGSVVSQAEEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDI RLIVDVGSIDEKDNESGVAHMVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYER TMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERKIILEEWRGKLGVAERMNQQR VQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREI QRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRH RLLTQITMSAVTRQVRRQKAELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISA VLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETREFADWVQQLTIVWQQDRPYVG SQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQW ALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGK KVYLTATSQAGFMATAMNPWQAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEAD QLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMSLARQKANDDQSVGGKRASEM TKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLA TIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIAS KYLKTSLRDDALGIYRMRVDSELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTK ITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYNDPRYLSNASKLADSITLESVR AMSAKLYNPDNRVLYITLPQEVKE
experiment.sample.molecule[0].mw	101.253
experiment.sample.molecule[0].total_mw	101.253
experiment.sample.molecule[0].number_molecules	1
experiment.sample.molecule[0].complex_state	False
experiment.sample.molecule[0].deuteration	null
experiment.sample.molecule[0].molecule_source	biological
experiment.sample.molecule[0].molecule_description	null
experiment.sample.buffer.name	20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol
experiment.sample.buffer.concentration_unit	null
experiment.sample.buffer.comment	null
experiment.sample.buffer.additive	null
experiment.sample.buffer.concentration	null
experiment.sample.buffer.pka	null
experiment.sample.buffer.ph	7.8
experiment.sample.buffer.deuteration	null
experiment.sample.purity_method	null
experiment.sample.name	The ferredoxin protease, FusC
experiment.sample.ext_coefficient	null
experiment.sample.contrast	null
experiment.sample.specific_vol	null
experiment.sample.dry_vol	null
experiment.sample.absorbption	null
experiment.sample.deuteration	null
experiment.sample.mixture	null
experiment.contributor[0].affiliation[0].short_name	null
experiment.contributor[0].affiliation[0].address	Melbourne, Australia
experiment.contributor[0].affiliation[0].full_name	Monash University
experiment.contributor[0].affiliation[0].webpage	http://www.monash.edu/
experiment.contributor[0].contributor_name	Grinter
experiment.contributor[0].contributor_surname	Grinter
experiment.contributor[0].orcid	https://orcid.org/0000-0002-8195-5348
experiment.concentration_method	null
experiment.concentration_unit	null
experiment.date	2017-04-06
experiment.storage_temperature	20.0
experiment.cell_temperature	20.0
experiment.exposure_time	null
experiment.number_of_frames	null
experiment.wavelength	0.103
experiment.sample_detector_distance	2.68
experiment.concentration_min	null
experiment.concentration_max	10.0
experiment.sample_volume	null
experiment.flow_rate	null
experiment.s_min	0.064
experiment.s_max	2.79
experiment.total_exposure_time	null
experiment.seccolumn	null
fits	[]
estimated_volume_method	null
pddf_software	ATSAS GNOM
pddf_software_version	5.0
i0_calibration_standard	null
description	Additional SEC parameters: Column type: GE Healthcare Superdex S200 10/300; Flow rate: 0.4 ml/min; Sample injection concentration: 10 mg/ml; Injection volume: 100 µl.
experiment_description	Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of ferredoxin protease, FusC, using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) at the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia). Data were collected a using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm. The SEC mobile phase consisted of 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % v/v glycerol, pH 7.8, (20°C). The SAXS data data measured from the SEC-elution (sample peak and buffer) were normalized to the intensity of the transmitted beam and radially averaged. The data from the sample SEC-peak were scaled and averaged and the scattering of the solvent-blank was subtracted from the sample frames to produce the data displayed in this entry.
tags	[]
intensity_unit	arbitrary
experimental_mw	100.0
experimental_mw_error	null
guinier_i0_mw	null
guinier_i0_mw_error	null
porod_mw	89.2
porod_mw_error	null
pddf_i0	0.1299
pddf_i0_error	null
guinier_i0	0.13
guinier_i0_error	0.00011
pddf_rg	3.74
pddf_rg_error	null
guinier_rg	3.68
guinier_rg_error	0.013
pddf_dmax	12.84
pddf_dmax_error	null
porod_volume	151.6
porod_volume_error	null
estimated_volume	null
estimated_volume_error	null
guinier_point_first	9
guinier_point_last	74
pddf_point_first	null
pddf_point_last	null
i0_calibration_standard_data	null
intensities_log_log_plot	SASDDQ6_datloglog_img.png
symmetry	null
last_modified	2020-04-22T08:28:46.473948+02:00
bragg_peak	[]

manifest.json：36 field values

Field path	Raw value
code	SASDDQ6
status	success
started_at	2026-08-11T15:13:53.880760+00:00
finished_at	2026-08-11T15:14:01.802390+00:00
source_last_modified	2020-04-22T08:28:46.473948+02:00
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View the complete summary.json source

{
  "code": "SASDDQ6",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.",
    "publication": {
      "title": "FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.",
      "author_list": "Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T",
      "journal": "PLoS Biol",
      "doi": "10.1371/journal.pbio.2006026",
      "pmid": "30071011",
      "published_date": "2018 Aug"
    },
    "status": "released",
    "submitted_date": "2018-05-19",
    "released_date": "2018-06-13"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDQ6.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDQ6.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDQ6_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDQ6.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "Australian  Synchrotron",
      "city": "Melbourne",
      "country": "Australia",
      "beamline_name": "SAXS/WAXS",
      "beam_geometry": "Point",
      "type_of_source": "X-ray synchrotron",
      "point_source": true,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Ferredoxin Protease",
          "short_name": "FusC",
          "sequence": "EEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDIRLIVDVGSIDEKDNESGVAH\r\nMVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYERTMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERK\r\nIILEEWRGKLGVAERMNQQRVQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREI\r\nQRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRHRLLTQITMSAVTRQVRRQKA\r\nELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISAVLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETRE\r\nFADWVQQLTIVWQQDRPYVGSQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQW\r\nALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGKKVYLTATSQAGFMATAMNPW\r\nQAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEADQLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMS\r\nLARQKANDDQSVGGKRASEMTKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLA\r\nTIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIASKYLKTSLRDDALGIYRMRVD\r\nSELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTKITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYND\r\nPRYLSNASKLADSITLESVRAMSAKLYNPDNRVLYITLPQEVKE",
          "organism": "Pectobacterium atrosepticum SCRI1043",
          "uniprot_code": "Q6D8U3",
          "uniprot_range_first": 26,
          "uniprot_range_last": 924,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MNWKKCCWLSLTAVGLLAGSVVSQAEEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDI\nRLIVDVGSIDEKDNESGVAHMVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYER\nTMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERKIILEEWRGKLGVAERMNQQR\nVQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREI\nQRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRH\nRLLTQITMSAVTRQVRRQKAELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISA\nVLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETREFADWVQQLTIVWQQDRPYVG\nSQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQW\nALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGK\nKVYLTATSQAGFMATAMNPWQAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEAD\nQLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMSLARQKANDDQSVGGKRASEM\nTKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLA\nTIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIAS\nKYLKTSLRDDALGIYRMRVDSELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTK\nITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYNDPRYLSNASKLADSITLESVR\nAMSAKLYNPDNRVLYITLPQEVKE",
          "mw": 101.253,
          "total_mw": 101.253,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.8,
        "deuteration": null
      },
      "purity_method": null,
      "name": "The ferredoxin protease, FusC",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Melbourne, Australia",
            "full_name": "Monash University",
            "webpage": "http://www.monash.edu/"
          }
        ],
        "contributor_name": "Grinter",
        "contributor_surname": "Grinter",
        "orcid": "https://orcid.org/0000-0002-8195-5348"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-04-06",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": 0.103,
    "sample_detector_distance": 2.68,
    "concentration_min": null,
    "concentration_max": 10.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.064,
    "s_max": 2.79,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "Additional SEC parameters:\r\nColumn type: GE Healthcare Superdex S200 10/300; Flow rate: 0.4 ml/min; Sample injection concentration: 10 mg/ml; Injection volume: 100 µl.",
  "experiment_description": "Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of ferredoxin protease, FusC, using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) at the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia). Data were collected a using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm. The SEC mobile phase consisted of 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % v/v glycerol, pH 7.8, (20°C). The SAXS data data measured from the SEC-elution (sample peak and buffer) were normalized to the intensity of the transmitted beam and radially averaged. The data from the sample SEC-peak were scaled and averaged and the scattering of the solvent-blank was subtracted from the sample frames to produce the data displayed in this entry.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 100.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 89.2,
  "porod_mw_error": null,
  "pddf_i0": 0.1299,
  "pddf_i0_error": null,
  "guinier_i0": 0.13,
  "guinier_i0_error": 0.00011,
  "pddf_rg": 3.74,
  "pddf_rg_error": null,
  "guinier_rg": 3.68,
  "guinier_rg_error": 0.013,
  "pddf_dmax": 12.84,
  "pddf_dmax_error": null,
  "porod_volume": 151.6,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 9,
  "guinier_point_last": 74,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDDQ6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-04-22T08:28:46.473948+02:00",
  "bragg_peak": []
}

View the complete manifest.json source

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Title	FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.
Authors	Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
DOI / PMID	10.1371/journal.pbio.2006026 / 30071011