Human metaphase chromosomes in 5 mM magnesium chloride
Data type: SASBDB experimental dataStatus: PublishedCurve type: Single concentrationLast updated: 2023-12-07T16:12:46.422621+01:00
Note: As the sample used for the SAXS measurements consists of intact chromosomes, the MW, Rg and sample concentration cannot be determined. Although the data for this entry are displayed as logI(s) vs s, by convention in the field a plot of log(I(s)s^2) vs s is used. This transformed I(s) data is included as an additional file in the full entry zip archive.
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1. Samples, Components and Experimental Conditions Sample & Experiment
Sample 1 · Human metaphase chromosomes in 5 mM magnesium chloride
Concentration
— – —
Buffer / pH
10 mM PIPES, 5 mM magnesium chloride, 10 mM sodium chloride, 120 mM potassium chloride, 40% glycerol / 7.2
Experimental temperature
25.0
Facility / beamline
ALBA / BL11 - NCD
Wavelength
0.129 nm
Exposure
60.0 s × 1
Molecular Components
Component
Type / organism
UniProt and Construct
Oligomeric State
Molecular weight
Human metaphase chromosomes in 5 mM magnesium chlorideView sequence
GGG
otherHomo sapiens
——–—
monomerMolecule count 1
0.001 kDa
Experimental Curves
Curve
Points / columns
q range
Uncertainty quality
Negative-intensity points
Source file
1
1511[2]
0.00105–3.173 1/nm
No uncertainty columnMissing 1511
0
sasbdb/entries/r5/sasddr5/source/SASDDR5.dat
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2. Measurements Reported by SASBDB Reported Results
Metric
Method
Value
Uncertainty
Unit
mw
Experimental
0.0
—
kDa
rg
P(r)
3.0
—
nm
These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.
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3. Source Fits and Models Source Fits & Models
This entry has no source fit record.
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4. Source File Index Source Files
Category
Path
Download status
Size
curve
sasbdb/entries/r5/sasddr5/source/SASDDR5.dat
downloaded
60458
full_entry_zip
sasbdb/entries/r5/sasddr5/source/SASDDR5.zip
downloaded
21957
pddf
sasbdb/entries/r5/sasddr5/source/SASDDR5.out
not_listed
—
sascif
sasbdb/entries/r5/sasddr5/source/SASDDR5.sascif
downloaded
86615
summary
sasbdb/entries/r5/sasddr5/source/summary.json
downloaded
6217
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5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
Synchrotron SAXS data from solutions of human metaphase chromosomes in 5 mM magnesium chloride, 10 mM PIPES, 10 mM sodium chloride, 120 mM potassium chloride, 40 %v/v glycerol, pH 7.2 were collected on the BL11-NCD beam line at the ALBA storage ring (Cerdanyola del Vallès, Barcelona, Spain) using a CCD ADSC Quantum 210r detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.129 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Sample data were collected at 25°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Related Publication
Title
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure.
Authors
Chicano A, Crosas E, Otón J, Melero R, Engel BD, Daban JR
DOI / PMID
10.15252/embj.201899769 / 30609992
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Note: As the sample used for the SAXS measurements consists of intact chromosomes, the MW, Rg and sample concentration cannot be determined. Although the data for this entry are displayed as logI(s) vs s, by convention in the field a plot of log(I(s)s^2) vs s is used. This transformed I(s) data is included as an additional file in the full entry zip archive.
experiment_description
Synchrotron SAXS data from solutions of human metaphase chromosomes in 5 mM magnesium chloride, 10 mM PIPES, 10 mM sodium chloride, 120 mM potassium chloride, 40 %v/v glycerol, pH 7.2 were collected on the BL11-NCD beam line at the ALBA storage ring (Cerdanyola del Vallès, Barcelona, Spain) using a CCD ADSC Quantum 210r detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.129 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Sample data were collected at 25°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.