SASDDS2

EspG3 chaperone from Mycobacter smegmatis

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · EspG3 chaperone from Mycobacter smegmatis

Concentration— – 0.78 Buffer / pH20 mM HEPES pH 7.5, 150 mM NaCl / 7.5
Experimental temperature— Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
EspG3 chaperone from Mycobacterium smegmatis
View sequence
GHMGPNAVELTTDQAWCLADVLGAGSYPWVLAITPPYSDHSQRSAFLAAQSAELTRMGVVNSAGAVDPRVAQWITTVCRATQWLDLRFVSGPGDLLRGMVARRSEETVVALRNAQLVTFTAMDIGHQHALVPVLTAGLSGRKPARFDDFALPAAAGARADEQIRNGAPLAEVLEFLGVPPSARPLVESVFDGRRTYVEIVAGEHRDGHRVTTEVGVSIIDTPHGRILVHPTKAFDGEWISTFTPGSADAIAMAVERLTASLPSGSWFPDQPLTRDFDEDAATHREPVLQRRTQKA
proteinMycobacterium smegmatisA0QQ45—–—monomerMolecule count 131.8 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11132[3]0.0999299–4.0086 1/nmHas uncertainty columnMissing 00sasbdb/entries/s2/sasdds2/source/SASDDS2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)8.56—nm
i0Guinier0.00172.5e-05arbitrary
i0P(r)0.001648—arbitrary
mwExperimental21.0—kDa
rgGuinier2.50.1nm
rgP(r)2.57—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES pH 7.5, 150 mM NaClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2014-03-17Storage / measurement temperature— / —
Exposure time0.05Frames20
Wavelength0.124Sample-to-detector distance—
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.1 – 4.009Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDDS2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
project.publication.titleStructural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
project.publication.author_listTuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
project.publication.journalnull
project.publication.doi10.1101/250803
project.publication.pmidnull
project.publication.published_date2018 Jan 24
project.statusreleased
project.submitted_date2018-01-09
project.released_date2018-01-24
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEspG3 chaperone from Mycobacterium smegmatis
experiment.sample.molecule[0].short_nameEspG3Msm
experiment.sample.molecule[0].sequenceGHMGPNAVELTTDQAWCLADVLGAGSYPWVLAITPPYSDHSQRSAFLAAQSAELTRMGVVNSAGAVDPRVAQWITTVCRATQWLDLRFVSGPGDLLRGMVARRSEETVVALRNAQLVTFTAMDIGHQHALVPVLTAGLSGRKPARFDDFALPAAAGARADEQIRNGAPLAEVLEFLGVPPSARPLVESVFDGRRTYVEIVAGEHRDGHRVTTEVGVSIIDTPHGRILVHPTKAFDGEWISTFTPGSADAIAMAVERLTASLPSGSWFPDQPLTRDFDEDAATHREPVLQRRTQKA
experiment.sample.molecule[0].organismMycobacterium smegmatis
experiment.sample.molecule[0].uniprot_codeA0QQ45
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGPNAVELTTDQAWCLADVLGAGSYPWVLAITPPYSDHSQRSAFLAAQSAELTRMGVVNS AGAVDPRVAQWITTVCRATQWLDLRFVSGPGDLLRGMVARRSEETVVALRNAQLVTFTAM DIGHQHALVPVLTAGLSGRKPARFDDFALPAAAGARADEQIRNGAPLAEVLEFLGVPPSA RPLVESVFDGRRTYVEIVAGEHRDGHRVTTEVGVSIIDTPHGRILVHPTKAFDGEWISTF TPGSADAIAMAVERLTASLPSGSWFPDQPLTRDFDEDAATHREPVLQRRTQKA
experiment.sample.molecule[0].mw31.8
experiment.sample.molecule[0].total_mw31.8
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES pH 7.5, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEspG3 chaperone from Mycobacter smegmatis
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAnne
experiment.contributor[0].contributor_surnameTuukkanen
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-03-17
experiment.storage_temperaturenull
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experiment.exposure_time0.05
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experiment.s_min0.1
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experiment_descriptionnull
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symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDDS2
statussuccess
started_at2026-08-11T13:44:53.796968+00:00
finished_at2026-08-11T13:45:02.190591+00:00
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View the complete summary.json source
{
  "code": "SASDDS2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems",
    "publication": {
      "title": "Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems",
      "author_list": "Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K",
      "journal": null,
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      "pmid": null,
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    },
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}
View the complete manifest.json source
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