Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)

{
  "code": "SASDDT5",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Stability of local secondary structure determines selectivity of viral RNA chaperones.",
    "publication": {
      "title": "Stability of local secondary structure determines selectivity of viral RNA chaperones.",
      "author_list": "Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gky394",
      "pmid": "29796667",
      "published_date": "2018 May 18"
    },
    "status": "released",
    "submitted_date": "2018-04-01",
    "released_date": "2018-04-26"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDT5.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDT5.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT5_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT5_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDT5_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDT5.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": "1 x 5 mm",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Nonstructural protein sigma NS",
          "short_name": null,
          "sequence": "MDNTVRVGVSRNTSGAAGQTLFRNFYLLRCNISADGRNATKAVQSHFPFLSRAVRCLSPL\r\nAAHCADRTLRRDNVKQILTRELPFSSDLINYAHHVNSSSLTTSQGVEAARLVAQVYGEQV\r\nPFDHIYPTGSATYCPGAIANAISRIMAGFVPREGDDFAPSGPIDYLAADLIAYKFVLPYM\r\nLDMVDGRPQIVLPSHTVEEMLTNTSLLNSIDASFGIEARSDQRMTRDAAEMSSRSLNELE\r\nDHDQRGRMPWKIMLAMMAAQLKVELDALADERTESQANAHVTSFGSRLFNQMSAFVTIDR\r\nELMELALLIKEQGFAMNPGQIASKWSLIRRSGPTRPLSGARLEIRNGNWMIREGDQTLLS\r\nVSPARMA",
          "organism": "Avian orthoreovirus",
          "uniprot_code": "Q9DH28",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "octamer",
          "molecular_type": "protein",
          "uniprot_sequence": "MDNTVRVGVSRNTSGAAGQTLFRNFYLLRCNISADGRNATKAVQSHFPFLSRAVRCLSPL\nAAHCADRTLRRDNVKQILTRELPFSSDLINYAHHVNSSSLTTSQGVEAARLVAQVYGEQV\nPFDHIYPTGSATYCPGAIANAISRIMAGFVPREGDDFAPSGPIDYLAADLIAYKFVLPYM\nLDMVDGRPQIVLPSHTVEEMLTNTSLLNSIDASFGIEARSDQRMTRDAAEMSSRSLNELE\nDHDQRGRMPWKIMLAMMAAQLKVELDALADERTESQANAHVTSFGSRLFNQMSAFVTIDR\nELMELALLIKEQGFAMNPGQIASKWSLIRRSGPTRPLSGARLEIRNGNWMIREGDQTLLS\nVSPARMA",
          "mw": 40.586,
          "total_mw": 324.688,
          "number_molecules": 8,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "20mer RNA (unstructured)",
          "short_name": "20mer",
          "sequence": "CUUUUCAAGACAUGCAACAA",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "RNA",
          "uniprot_sequence": null,
          "mw": 6.399,
          "total_mw": 12.798,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM HEPES, 150 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Leeds, UK",
            "full_name": "University of Leeds",
            "webpage": "https://www.leeds.ac.uk/"
          }
        ],
        "contributor_name": "Jack",
        "contributor_surname": "Bravo",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-02-25",
    "storage_temperature": null,
    "cell_temperature": null,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": 0.1,
    "sample_detector_distance": 3.9,
    "concentration_min": null,
    "concentration_max": null,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.037,
    "s_max": 3.7,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "SigmaNS ribonucleoprotein complex with 20mer RNA. Sample concentration, UNKNOWN; Sample temperature, UNKNOWN; Exposure time; UNKNOWN.",
  "experiment_description": "Synchrotron SAXS data from solutions of ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20) in 25 mM HEPES, 150 mM NaCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 323.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 0.3067,
  "pddf_i0_error": null,
  "guinier_i0": 0.2991,
  "guinier_i0_error": 0.001,
  "pddf_rg": 8.65,
  "pddf_rg_error": null,
  "guinier_rg": 7.82,
  "guinier_rg_error": 0.06,
  "pddf_dmax": 38.0,
  "pddf_dmax_error": null,
  "porod_volume": 964.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 11,
  "guinier_point_last": 45,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDDT5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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