SASDDU2

EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)

浓度— – 3.79 缓冲液 / pH20 mM HEPES pH 7.5, 150 mM NaCl / 7.5
实验温度— 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
EspG3 chaperone from Mycobacterium smegmatis
查看序列
GHMGPNAVELTTDQAWCLADVLGAGSYPWVLAITPPYSDHSQRSAFLAAQSAELTRMGVVNSAGAVDPRVAQWITTVCRATQWLDLRFVSGPGDLLRGMVARRSEETVVALRNAQLVTFTAMDIGHQHALVPVLTAGLSGRKPARFDDFALPAAAGARADEQIRNGAPLAEVLEFLGVPPSARPLVESVFDGRRTYVEIVAGEHRDGHRVTTEVGVSIIDTPHGRILVHPTKAFDGEWISTFTPGSADAIAMAVERLTASLPSGSWFPDQPLTRDFDEDAATHREPVLQRRTQKA
proteinMycobacterium smegmatisA0QQ45—–—monomer分子数 131.8 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11429[3]0.0779402–4.01135 1/nm含误差列缺失 00sasbdb/entries/u2/sasddu2/source/SASDDU2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.2—nm
i0Guinier0.0019323.2e-06arbitrary
i0P(r)0.001931—arbitrary
mwExperimental24.3—kDa
rgGuinier2.60.06nm
rgP(r)2.623—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES pH 7.5, 150 mM NaCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2014-03-17储存 / 测量温度— / —
曝光时间0.05帧数20
波长0.124样品-探测器距离—
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.078 – 4.011样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDDU2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
project.publication.titleStructural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
project.publication.author_listTuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K
project.publication.journalnull
project.publication.doi10.1101/250803
project.publication.pmidnull
project.publication.published_date2018 Jan 24
project.statusreleased
project.submitted_date2018-01-09
project.released_date2018-01-24
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEspG3 chaperone from Mycobacterium smegmatis
experiment.sample.molecule[0].short_nameEspG3Msm
experiment.sample.molecule[0].sequenceGHMGPNAVELTTDQAWCLADVLGAGSYPWVLAITPPYSDHSQRSAFLAAQSAELTRMGVVNSAGAVDPRVAQWITTVCRATQWLDLRFVSGPGDLLRGMVARRSEETVVALRNAQLVTFTAMDIGHQHALVPVLTAGLSGRKPARFDDFALPAAAGARADEQIRNGAPLAEVLEFLGVPPSARPLVESVFDGRRTYVEIVAGEHRDGHRVTTEVGVSIIDTPHGRILVHPTKAFDGEWISTFTPGSADAIAMAVERLTASLPSGSWFPDQPLTRDFDEDAATHREPVLQRRTQKA
experiment.sample.molecule[0].organismMycobacterium smegmatis
experiment.sample.molecule[0].uniprot_codeA0QQ45
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGPNAVELTTDQAWCLADVLGAGSYPWVLAITPPYSDHSQRSAFLAAQSAELTRMGVVNS AGAVDPRVAQWITTVCRATQWLDLRFVSGPGDLLRGMVARRSEETVVALRNAQLVTFTAM DIGHQHALVPVLTAGLSGRKPARFDDFALPAAAGARADEQIRNGAPLAEVLEFLGVPPSA RPLVESVFDGRRTYVEIVAGEHRDGHRVTTEVGVSIIDTPHGRILVHPTKAFDGEWISTF TPGSADAIAMAVERLTASLPSGSWFPDQPLTRDFDEDAATHREPVLQRRTQKA
experiment.sample.molecule[0].mw31.8
experiment.sample.molecule[0].total_mw31.8
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES pH 7.5, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAnne
experiment.contributor[0].contributor_surnameTuukkanen
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-03-17
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max3.79
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.078
experiment.s_max4.011
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw24.3
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.001931
pddf_i0_errornull
guinier_i00.001932
guinier_i0_error3.2e-06
pddf_rg2.623
pddf_rg_errornull
guinier_rg2.6
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pddf_dmax9.2
pddf_dmax_errornull
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guinier_point_first5
guinier_point_last150
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDU2_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDU2
statussuccess
started_at2026-08-11T13:46:16.892016+00:00
finished_at2026-08-11T13:46:27.499551+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDDU2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems",
    "publication": {
      "title": "Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems",
      "author_list": "Tuukkanen A, Freire D, Chan S, Arbing M, Reed R, Evans T, Zenkeviciutė G, Kim J, Kahng S, Sawaya M, Chaton C, Wilmanns M, Eisenberg D, Parret A, Korotkov K",
      "journal": null,
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      "pmid": null,
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    },
    "status": "released",
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}
查看完整 manifest.json 原文
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