SASDDU4

Fc region of Immunoglobulin G2 (IgG2 Fc)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Fc region of Immunoglobulin G2 (IgG2 Fc)

浓度1.0 – 12.0 缓冲液 / pH20mM HEPES, 50mM NaCl / 7.5
实验温度20.0 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.103 nm曝光0.3 s × 33

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Immunoglobulin heavy constant gamma 2
查看序列
VECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
proteinHomo sapiensP01859—–—dimer分子数 226.2 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1942[3]0.01477–0.53968 1/A含误差列缺失 00sasbdb/entries/u4/sasddu4/source/SASDDU4.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.00.01Å
i0Guinier1.0580.001arbitrary
i0P(r)1.0430.003arbitrary
mwExperimental51.0—kDa
porod_volumePorod67.0—ų
rgGuinier2.770.005Å
rgP(r)2.70.003Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20mM HEPES, 50mM NaCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-02-17储存 / 测量温度4.0 / 20.0
曝光时间0.3帧数33
波长0.103样品-探测器距离1.5
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q 范围0.148 – 5.397样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDDU4
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleConformational Plasticity of the Immunoglobulin Fc Domain in Solution.
project.publication.titleConformational Plasticity of the Immunoglobulin Fc Domain in Solution.
project.publication.author_listRemesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M
project.publication.journalStructure
project.publication.doi10.1016/j.str.2018.03.017
project.publication.pmid29731233
project.publication.published_date2018 Jul 3
project.statusreleased
project.submitted_date2018-03-06
project.released_date2018-04-10
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experiment.instrument.detector.typenull
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
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experiment.sample.molecule[0].long_nameImmunoglobulin heavy constant gamma 2
experiment.sample.molecule[0].short_nameIgG2 Fc S257A
experiment.sample.molecule[0].sequenceVECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPR EEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTK NQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM HEALHNHYTQKSLSLSPGK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP01859
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].total_mw52.4
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20mM HEPES, 50mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFc region of Immunoglobulin G2 (IgG2 Fc)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameUCSF
experiment.contributor[0].affiliation[0].addressSan Francisco, CA, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of California, San Francisco (UCSF)
experiment.contributor[0].affiliation[0].webpagehttps://www.ucsf.edu/
experiment.contributor[0].contributor_nameSoumya
experiment.contributor[0].contributor_surnameG Remesh
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-02-17
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time0.3
experiment.number_of_frames33
experiment.wavelength0.103
experiment.sample_detector_distance1.5
experiment.concentration_min1.0
experiment.concentration_max12.0
experiment.sample_volumenull
experiment.flow_ratenull
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experiment.s_max5.397
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareBILBOMD
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
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fits[0].softwareMultiFoXS
fits[0].chi_square_value2.998126
fits[0].p_value0.0
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of the Fc region of Immunoglobulin G2 (IgG2 Fc) in 20mM HEPES, 50mM NaCl, pH 7.5, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 12 mg/ml were measured at 20°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unitarbitrary
experimental_mw51.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i01.043
pddf_i0_error0.003
guinier_i01.058
guinier_i0_error0.001
pddf_rg2.7
pddf_rg_error0.003
guinier_rg2.77
guinier_rg_error0.005
pddf_dmax9.0
pddf_dmax_error0.01
porod_volume67.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last60
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDU4_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDU4
statussuccess
started_at2026-08-11T14:31:23.065446+00:00
finished_at2026-08-11T14:31:34.653058+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDDU4",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Conformational Plasticity of the Immunoglobulin Fc Domain in Solution.",
    "publication": {
      "title": "Conformational Plasticity of the Immunoglobulin Fc Domain in Solution.",
      "author_list": "Remesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M",
      "journal": "Structure",
      "doi": "10.1016/j.str.2018.03.017",
      "pmid": "29731233",
      "published_date": "2018 Jul 3"
    },
    "status": "released",
    "submitted_date": "2018-03-06",
    "released_date": "2018-04-10"
  },
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          "short_name": "IgG2 Fc S257A",
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查看完整 manifest.json 原文
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