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{
"code": "SASDDU8",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains.",
"publication": {
"title": "Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains.",
"author_list": "Huang W, Peng Y, Kiselar J, Zhao X, Albaqami A, Mendez D, Chen Y, Chakravarthy S, Gupta S, Ralston C, Kao HY, Chance MR, Yang S",
"journal": "Nat Commun",
"doi": "10.1038/s41467-018-06034-2",
"pmid": "30166540",
"published_date": "2018 Aug 30"
},
"status": "released",
"submitted_date": "2018-06-22",
"released_date": "2018-09-01"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDU8.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDU8.dat",
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"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDU8_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDU8_pofr_img.png",
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"experiment": {
"instrument": {
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"type": null,
"name": "MAR 165 CCD",
"resolution": null
},
"name": "Advanced Photon Source (APS), Argonne National Laboratory",
"city": "Lemont, IL",
"country": "USA",
"beamline_name": "BioCAT 18ID",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
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"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Estrogen receptor",
"short_name": "hERa CDE",
"sequence": "ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC\r\nRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR\r\nSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW\r\nAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG\r\nMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD\r\nKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLL\r\nLEMLDAHRLHAP",
"organism": null,
"uniprot_code": "P03372",
"uniprot_range_first": 181,
"uniprot_range_last": 552,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAY\nEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPF\nLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK\nETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC\nRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR\nSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW\nAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG\nMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD\nKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLL\nLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV",
"mw": 42.468,
"total_mw": 84.936,
"number_molecules": 2,
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"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "ERE1",
"short_name": "hERa_ERE1",
"sequence": "TAGGTACACGTGACCTGCG",
"organism": "Homo sapiens",
"uniprot_code": null,
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "monomer",
"molecular_type": "DNA",
"uniprot_sequence": null,
"mw": 5.909,
"total_mw": 5.909,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "ERE2",
"short_name": "hERa_ERE2",
"sequence": "CGCAGGTCACTGTGACCTA",
"organism": "Homo sapiens",
"uniprot_code": null,
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"oligomerization": "monomer",
"molecular_type": "DNA",
"uniprot_sequence": null,
"mw": 5.869,
"total_mw": 5.869,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Estradiol",
"short_name": null,
"sequence": "C18H24O2",
"organism": null,
"uniprot_code": null,
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "dimer",
"molecular_type": "other",
"uniprot_sequence": null,
"mw": 0.272,
"total_mw": 0.272,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "hERa peptide1",
"short_name": null,
"sequence": "KENALLRYLLDKDD",
"organism": null,
"uniprot_code": null,
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": null,
"mw": 1.706,
"total_mw": 1.706,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "hERa peptide2",
"short_name": null,
"sequence": "KENALLRYLLDKDD",
"organism": null,
"uniprot_code": null,
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": null,
"mw": 1.706,
"total_mw": 1.706,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "10 mM CHES (pH9.5), 125 mM NaCl, 5mM KCl, 4 mM MgCl2, 50 mM arginine, 50 mM glutamate, 5 mM TCEP, 5% glycerol, 10 µm Zn acetate, 10 µM estradiol",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 9.5,
"deuteration": null
},
"purity_method": null,
"name": "Multidomain architecture of the estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Cleveland, USA",
"full_name": "Case Western Reserve University",
"webpage": "https://case.edu/"
}
],
"contributor_name": "Sichun",
"contributor_surname": "Yang",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2014-08-10",
"storage_temperature": 4.0,
"cell_temperature": 10.0,
"exposure_time": 1.1,
"number_of_frames": 20,
"wavelength": 0.103,
"sample_detector_distance": 3.0,
"concentration_min": 0.1,
"concentration_max": 0.8,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.171,
"s_max": 2.468,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDU8_fit1_model1_img.png",
"software": "Custom in-house",
"pdb_link": [],
"model_title": null,
"type_of_model": "atomic",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDU8_fit1_model1.pdb",
"model_mw": 207.1,
"bead_radius": null,
"log": null,
"symmetry": null,
"comment": null,
"user": 312
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDU8_fit1_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 1.378276,
"p_value": 0.108697,
"fit_residual_plot": "SASDDU8_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDU8_fit1.fit",
"fit_log": null,
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"description": null
},
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDU8_fit2_model1_img.png",
"software": "Custom in-house",
"pdb_link": [],
"model_title": null,
"type_of_model": "atomic",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDU8_fit2_model1.pdb",
"model_mw": 207.1,
"bead_radius": null,
"log": null,
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"comment": null,
"user": 312
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDU8_fit2_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 1.194649,
"p_value": 0.389292,
"fit_residual_plot": "SASDDU8_fit2_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDU8_fit2.fit",
"fit_log": null,
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},
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDU8_fit3_model1_img.png",
"software": "Custom in-house",
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"model_title": null,
"type_of_model": "atomic",
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"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDU8_fit3_model1.pdb",
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}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDU8_fit3_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 1.245456,
"p_value": 0.211077,
"fit_residual_plot": "SASDDU8_fit3_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDU8_fit3.fit",
"fit_log": null,
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}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "4.6",
"i0_calibration_standard": null,
"description": "Additional SEC parameters: \r\nColumn type: GE Healthcare Superdex 200 GL 10/300;\r\nFlow rate: 0.5 ml/min; \r\nSample injection concentration: 3.5 mg/ml;\r\nInjection volume: 500 µl.",
"experiment_description": "Synchrotron SAXS data from solutions of the multi-component estrogen receptor in 10 mM CHES, 125 mM NaCl, 5mM KCl, 4 mM MgCl2, 50 mM arginine, 50 mM glutamate, 5 mM TCEP, 5% glycerol, 10 µm Zn acetate, 10 µM estradiol, pH 9.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne, IL, USA) using a MAR 165 CCD detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.1 and 0.8 mg/ml were measured at 10°C from size-exclusion chromatography eluates. 20 successive 1.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 98.3,
"experimental_mw_error": null,
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"estimated_volume": null,
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"guinier_point_first": 1,
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"pddf_point_first": null,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDDU8_datloglog_img.png",
"symmetry": null,
"last_modified": "2019-12-06T13:07:15.624532+01:00",
"bragg_peak": []
}
