SASDDZ8

Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2021-08-18T17:32:55.174589+02:00

The ab initio dummy atom bead models displayed in this entry are: Top - a refined DAMMIN model and corresponding fit generated from 20 individual model spatial alignments; Bottom - the bead-occupancy and volume-corrected (averaged) spatial representation of TIP60 (DAMFILT) obtained from 20 individual model spatial alignments.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

浓度0.98 – 4.97 缓冲液 / pH25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol / 8.0
实验温度25.0 设备 / 束线Photon Factory (PF), High Energy Accelerator Research Organization (KEK) / BL-6A
波长0.15 nm曝光5.0 s × 30

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Fusion protein of LSm and MyoX-coil
查看序列
MGSSHHHHHHSQDPKNIKIMRLVTGEDIIGNISESQGLITIKKAFVIIPMQATPGKPVQLVLSPWQPYTDDKEIVIDDSKVITITSPKDDIIKSYESHTRVLENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQERRDQELRRLEEE
proteinArtificial protein——–—other分子数 6017.766 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1717[3]0.0569788–2.60301 1/nm含误差列缺失 00sasbdb/entries/z8/sasddz8/source/SASDDZ8.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)23.0—nm
i0Guinier0.6430.0011/cm
i0P(r)0.6241—1/cm
mwExperimental1063.0—kDa
mwGuinier I(0)1063.0—kDa
rgGuinier9.390.06nm
rgP(r)8.952—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-11-11储存 / 测量温度— / 25.0
曝光时间5.0帧数30
波长0.15样品-探测器距离2.023
光源X-ray synchrotron探测器Pilatus3 1M
机构 / 束线Photon Factory (PF), High Energy Accelerator Research Organization (KEK) / BL-6A · Tsukuba, Japan
q 范围0.057 – 2.603样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:169 个字段值
字段路径原始值
codeSASDDZ8
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleDesign of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces.
project.publication.titleDesign of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces.
project.publication.author_listKawakami N, Kondo H, Matsuzawa Y, Hayasaka K, Nasu E, Sasahara K, Arai R, Miyamoto K
project.publication.journalAngew Chem Int Ed Engl
project.publication.doi10.1002/anie.201805565
project.publication.pmid30066354
project.publication.published_date2018 Jul 31
project.statusreleased
project.submitted_date2018-06-24
project.released_date2018-08-03
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experiment.instrument.detector.namePilatus3 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.namePhoton Factory (PF), High Energy Accelerator Research Organization (KEK)
experiment.instrument.cityTsukuba
experiment.instrument.countryJapan
experiment.instrument.beamline_nameBL-6A
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experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw17.766
experiment.sample.molecule[0].total_mw1065.96
experiment.sample.molecule[0].number_molecules60
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experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressJapan
experiment.contributor[0].affiliation[0].full_nameShinshu University
experiment.contributor[0].affiliation[0].webpagehttps://www.shinshu-u.ac.jp
experiment.contributor[0].contributor_nameRyoichi
experiment.contributor[0].contributor_surnameArai
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experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-11-11
experiment.storage_temperaturenull
experiment.cell_temperature25.0
experiment.exposure_time5.0
experiment.number_of_frames30
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fits[0].chi_square_value2.976
fits[0].p_value1e-06
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fits[0].descriptionnull
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe ab initio dummy atom bead models displayed in this entry are: Top - a refined DAMMIN model and corresponding fit generated from 20 individual model spatial alignments; Bottom - the bead-occupancy and volume-corrected (averaged) spatial representation of TIP60 (DAMFILT) obtained from 20 individual model spatial alignments.
experiment_descriptionSynchrotron SAXS data from solutions of a designed hollow protein nanoparticle, TIP60, in 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH 8 were collected on the BL-6A beam line at the Photon Factory (PF), High Energy Acceleration Reserch Organization (KEK) storage ring (Tsukuba, Japan) using a PILATUS3 1M detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.15 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.0 and 5.0 mg/ml were measured at 25°C. 30 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
tags[]
intensity_unit1/cm
experimental_mw1063.0
experimental_mw_errornull
guinier_i0_mw1063.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.6241
pddf_i0_errornull
guinier_i00.643
guinier_i0_error0.001
pddf_rg8.952
pddf_rg_errornull
guinier_rg9.39
guinier_rg_error0.06
pddf_dmax23.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first4
guinier_point_last23
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDDZ8_datloglog_img.png
symmetrynull
last_modified2021-08-18T17:32:55.174589+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDDZ8
statussuccess
started_at2026-08-11T16:03:55.513650+00:00
finished_at2026-08-11T16:04:04.289191+00:00
source_last_modified2021-08-18T17:32:55.174589+02:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces.",
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      "title": "Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces.",
      "author_list": "Kawakami N, Kondo H, Matsuzawa Y, Hayasaka K, Nasu E, Sasahara K, Arai R, Miyamoto K",
      "journal": "Angew Chem Int Ed Engl",
      "doi": "10.1002/anie.201805565",
      "pmid": "30066354",
      "published_date": "2018 Jul 31"
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            "address": "Japan",
            "full_name": "Shinshu University",
            "webpage": "https://www.shinshu-u.ac.jp"
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  "last_modified": "2021-08-18T17:32:55.174589+02:00",
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}
查看完整 manifest.json 原文
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