C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism

{
  "code": "SASDE28",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
    "publication": {
      "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
      "author_list": "Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G",
      "journal": "Biophysical Journal",
      "doi": "10.1016/j.bpj.2019.12.025",
      "pmid": null,
      "published_date": "2020 Jan"
    },
    "status": "released",
    "submitted_date": "2018-11-13",
    "released_date": "2019-11-25"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDE28.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDE28.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE28_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE28_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE28_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE28_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE28.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Major prion protein",
          "short_name": null,
          "sequence": "GQGGSHSQWNKPSKPKTNMKHVAGAAAAGAVVGGLGGYMLGSVMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITVKQHTVQTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGAS",
          "organism": "Ovis aries",
          "uniprot_code": "P23907",
          "uniprot_range_first": 94,
          "uniprot_range_last": 234,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MVKSHIGSWILVLFVAMWSDVGLCKKRPKPGGGWNTGGSRYPGQGSPGGNRYPPQGGGGW\nGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGGWGQGGSHSQWNKPSKPKTNMKHVAGAAA\nAGAVVGGLGGYMLGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDRYSNQNNFVH\nDCVNITVKQHTVTTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGASVILFSS\nPPVILLISFLIFLIVG",
          "mw": 16.085,
          "total_mw": 16.085,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM Ammonium Acetate, 250 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 5.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "ESRF",
            "address": "Grenoble, France",
            "full_name": "European Synchrotron Radiation Facility",
            "webpage": "http://www.esrf.eu/"
          }
        ],
        "contributor_name": "Gabriele",
        "contributor_surname": "Giachin",
        "orcid": "https://orcid.org/0000-0001-7550-0307"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-05-04",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 1,
    "wavelength": 0.099,
    "sample_detector_distance": 2.864,
    "concentration_min": null,
    "concentration_max": 7.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.1,
    "s_max": 4.928,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDE28_fit1_model1_img.png",
          "software": "EOM/RANCH",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDE28_fit1_model1.pdb",
          "model_mw": 16.02,
          "bead_radius": null,
          "log": "https://www.sasbdb.org/media/log_files/logFile_001_1_17Xk3uh.log",
          "symmetry": "P1",
          "comment": null,
          "user": 388
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDE28_fit1_fixed_fit_img.png",
      "software": "EOM/GAJOE",
      "chi_square_value": 2.741,
      "p_value": 5.9e-05,
      "fit_residual_plot": "SASDE28_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDE28_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_2GztO3F.log",
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of the C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism in 25 mM ammonium acetate, 250 mM NaCl, pH 5.5 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column type, Superdex 200 5/150 GL (GE Healthcare); Flow rate: 0.2 mL/min; Total acquisition time: 25 min; Injection concentration: 7 mg/mL; Injection volume: 50 μL. The data obtained through the sample elution peak (630-670 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 16.1,
  "experimental_mw_error": null,
  "guinier_i0_mw": 15.2,
  "guinier_i0_mw_error": null,
  "porod_mw": 14.4,
  "porod_mw_error": null,
  "pddf_i0": 12.06,
  "pddf_i0_error": null,
  "guinier_i0": 11.69,
  "guinier_i0_error": 0.024,
  "pddf_rg": 2.311,
  "pddf_rg_error": null,
  "guinier_rg": 2.1,
  "guinier_rg_error": 0.01,
  "pddf_dmax": 9.1,
  "pddf_dmax_error": null,
  "porod_volume": 28.74,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 38,
  "guinier_point_last": 113,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDE28_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-08-28T15:28:10.263535+02:00",
  "bragg_peak": []
}

{
  "code": "SASDE28",
  "status": "success",
  "started_at": "2026-08-11T15:43:56.607726+00:00",
  "finished_at": "2026-08-11T15:44:08.114774+00:00",
  "source_last_modified": "2020-08-28T15:28:10.263535+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDE28/",
      "path": "/data/saxsdb/sasbdb/entries/28/sasde28/source/summary.json",
      "size": 7792,
      "sha256": "3bf5e40af3cb6a3270538e693fb81f115e402c667ccbc7e887bd4fe18c6adb1e"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDE28.dat",
      "path": "/data/saxsdb/sasbdb/entries/28/sasde28/source/SASDE28.dat",
      "size": 54728,
      "sha256": "1cab27fd79c2ddf22e75b401e8e3c110a200feb3bdba9c7a93f85e9aa96d9079",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDE28.out",
      "path": "/data/saxsdb/sasbdb/entries/28/sasde28/source/SASDE28.out",
      "size": 56764,
      "sha256": "9b0d1220517321fd3daebf188429385a35053fa00ea570fd09fac805a17deca3",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE28.sascif",
      "path": "/data/saxsdb/sasbdb/entries/28/sasde28/source/SASDE28.sascif",
      "size": 209997,
      "sha256": "9b8d752de44050a4ff87da6fae5955acb1da04cf0c18a2ba1bb04a4ea092442a",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDE28.zip",
      "path": "/data/saxsdb/sasbdb/entries/28/sasde28/source/SASDE28.zip",
      "size": 60060,
      "sha256": "bd14575433d7bb38d9077dde9770f31d1158a0a13e9dccdd4b8dcc6ff6957abb",
      "role": "full_entry_zip",
      "zip_member_count": 5,
      "zip_validation": "central_directory_readable"
    }
  ]
}