SASDE29

Interleukin-1 receptor accessory protein ectodomain with RI linker

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2020-11-27T12:02:24.063588+01:00

SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.

▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Interleukin-1 receptor accessory protein ectodomain with RI linker

Concentration— – 10.0 Buffer / pH10mM HEPES, 150mM NaCl, 3% glycerol / 7.2
Experimental temperature20.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.103 nmExposure3.0 s × 600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Interleukin-1 receptor accessory protein ectodomain with RI linker
View sequence
SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVLEENKPTRPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKAAALHHILDAQKMVWNHRHHHHHH
proteinHomo sapiensQ9NPH321–348monomerMolecule count 140.876 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1941[3]0.014215–0.538009 1/AHas uncertainty columnMissing 00sasbdb/entries/29/sasde29/source/SASDE29.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.39—Å
i0Guinier82.20.86arbitrary
i0P(r)82.55—arbitrary
mwExperimental49.8—kDa
porod_volumePorod76.0—ų
rgGuinier3.0370.04Å
rgP(r)3.04—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

▶

4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10mM HEPES, 150mM NaCl, 3% glycerolBuffer concentration—
pH7.2Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-08-11Storage / measurement temperature— / 20.0
Exposure time3.0Frames600
Wavelength0.103Sample-to-detector distance1.5
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.142 – 5.38Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
curvesasbdb/entries/29/sasde29/source/SASDE29.datdownloaded36595276e0483379834ffea97eb163b49414d6ef68fd5d6ca43770f038b0140d51dd4DownloadView source fileSource site
full_entry_zipsasbdb/entries/29/sasde29/source/SASDE29.zipdownloaded247207e9d0e694bb4aa2e6df051e3c17d3a5dc95bcb6f7c8f57453731079c552663cdbDownloadView source fileSource site
pddfsasbdb/entries/29/sasde29/source/SASDE29.outdownloaded37769852f2bbb56cf982148f9f8a1420be3393375f60da44c91a9bfbe55090570dd65DownloadView source fileSource site
sascifsasbdb/entries/29/sasde29/source/SASDE29.sascifdownloaded142493392c954a925b2d332e498b3bc725d95cc8839be6fbce8c9fe407e946540dedcf7DownloadView source fileSource site
summarysasbdb/entries/29/sasde29/source/summary.jsondownloaded88576481e3b9a0c1181133a43a154950c54ddb98c7827a51efdb018d66dd38b06224DownloadView source fileSource site
curve:source record
{
  "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.dat",
  "role": "curve",
  "sha256": "276e0483379834ffea97eb163b49414d6ef68fd5d6ca43770f038b0140d51dd4",
  "size": 36595,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDE29.dat"
}
full_entry_zip:source record and ZIP contents (5 items)
{
  "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.zip",
  "role": "full_entry_zip",
  "sha256": "e9d0e694bb4aa2e6df051e3c17d3a5dc95bcb6f7c8f57453731079c552663cdb",
  "size": 247207,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDE29.zip",
  "zip_member_count": 5,
  "zip_members": [
    {
      "crc32": "ae1c7bdf",
      "name": "SASDE29/experimental_data/SASDE29.dat",
      "size": 36595
    },
    {
      "crc32": "ac9a7999",
      "name": "SASDE29/pddf/SASDE29.out",
      "size": 37769
    },
    {
      "crc32": "30559887",
      "name": "SASDE29/SASDE29_fit1.dat",
      "size": 32870
    },
    {
      "crc32": "b346d35b",
      "name": "SASDE29/SASDE29_fit1_model1.pdb",
      "size": 493853
    },
    {
      "crc32": "9c6820e5",
      "name": "SASDE29/SASDE29_fit1_model2.pdb",
      "size": 493853
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:source record
{
  "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.out",
  "role": "pddf",
  "sha256": "852f2bbb56cf982148f9f8a1420be3393375f60da44c91a9bfbe55090570dd65",
  "size": 37769,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDE29.out"
}
sascif:source record
{
  "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.sascif",
  "role": "sascif",
  "sha256": "92c954a925b2d332e498b3bc725d95cc8839be6fbce8c9fe407e946540dedcf7",
  "size": 1424933,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE29.sascif"
}
summary:source record
{
  "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/summary.json",
  "sha256": "6481e3b9a0c1181133a43a154950c54ddb98c7827a51efdb018d66dd38b06224",
  "size": 8857,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDE29/"
}

All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:169 field values
Field pathRaw value
codeSASDE29
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleFunctional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.
project.publication.titleFunctional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.
project.publication.author_listGe J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X
project.publication.journalStructure
project.publication.doi10.1016/j.str.2019.05.011
project.publication.pmid31257107
project.publication.published_date2019 Aug 6
project.statusreleased
project.submitted_date2018-12-11
project.released_date2019-09-13
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDE29.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDE29.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE29_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE29_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE29_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE29_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDE29.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameInterleukin-1 receptor accessory protein ectodomain with RI linker
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceSERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVLEENKPTRPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKAAALHHILDAQKMVWNHRHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9NPH3
experiment.sample.molecule[0].uniprot_range_first21
experiment.sample.molecule[0].uniprot_range_last348
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTLLWCVVSLYFYGILQSDASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYST AHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTT YCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMG CYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVVGSPKNA VPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINE SISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQKVPAPRYTVEL ACGFGATVLLVVILIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNAEEEEF VLLTLRGVLENEFGYKLCIFDRDSLPGGIVTDETLSFIQKSRRLLVVLSPNYVLQGTQAL LELKAGLENMASRGNINVILVQYKAVKETKVKELKRAKTVLTVIKWKGEKSKYPQGRFWK QLQVAMPVKKSPRRSSSDEQGLSYSSLKNV
experiment.sample.molecule[0].mw40.876
experiment.sample.molecule[0].total_mw40.876
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10mM HEPES, 150mM NaCl, 3% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.2
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameInterleukin-1 receptor accessory protein ectodomain with RI linker
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressTaiwan
experiment.contributor[0].affiliation[0].full_nameNational Cheng Kung University
experiment.contributor[0].affiliation[0].webpagehttp://www.ncku.edu.tw/
experiment.contributor[0].contributor_nameShu-Ying
experiment.contributor[0].contributor_surnameWang
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-08-11
experiment.storage_temperaturenull
experiment.cell_temperature20.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.103
experiment.sample_detector_distance1.5
experiment.concentration_minnull
experiment.concentration_max10.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.142
experiment.s_max5.38
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDE29_fit1_model1_img.png
fits[0].models[0].softwareBILBOMD
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDE29_fit1_model1.pdb
fits[0].models[0].model_mw118.7
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user393
fits[0].models[1].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDE29_fit1_model2_img.png
fits[0].models[1].softwareBILBOMD
fits[0].models[1].pdb_link[]
fits[0].models[1].model_titlenull
fits[0].models[1].type_of_modelatomic
fits[0].models[1].software_versionnull
fits[0].models[1].model_datahttps://www.sasbdb.org/media/pdb_file/SASDE29_fit1_model2.pdb
fits[0].models[1].model_mw118.7
fits[0].models[1].bead_radiusnull
fits[0].models[1].lognull
fits[0].models[1].symmetrynull
fits[0].models[1].commentnull
fits[0].models[1].user393
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/multi_state_model_2_1_1_HTlPEKC_fitfoxs_img.png
fits[0].softwareMES-FoXS
fits[0].chi_square_value2.347585
fits[0].p_value0.0
fits[0].fit_residual_plotmulti_state_model_2_1_1_HTlPEKC_fitfoxsresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDE29_fit1.dat
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.5a
i0_calibration_standardnull
descriptionSEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.
experiment_descriptionSSynchrotron SAXS data from solutions of Interleukin-1 receptor accessory protein ectodomain with RI linker in 10mM HEPES, 150mM NaCl, 3% glycerol, pH 7.2 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected as 600 successive 3 second frames through the SEC elution profile. Sample-elution peak data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
tags[]
intensity_unitarbitrary
experimental_mw49.8
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i082.55
pddf_i0_errornull
guinier_i082.2
guinier_i0_error0.86
pddf_rg3.04
pddf_rg_errornull
guinier_rg3.037
guinier_rg_error0.04
pddf_dmax10.39
pddf_dmax_errornull
porod_volume76.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last48
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDE29_datloglog_img.png
symmetrynull
last_modified2020-11-27T12:02:24.063588+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDE29
statussuccess
started_at2026-08-11T16:44:14.079839+00:00
finished_at2026-08-11T16:44:23.629217+00:00
source_last_modified2020-11-27T12:02:24.063588+01:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDE29/
files[0].path/data/saxsdb/sasbdb/entries/29/sasde29/source/summary.json
files[0].size8857
files[0].sha2566481e3b9a0c1181133a43a154950c54ddb98c7827a51efdb018d66dd38b06224
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDE29.dat
files[1].path/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.dat
files[1].size36595
files[1].sha256276e0483379834ffea97eb163b49414d6ef68fd5d6ca43770f038b0140d51dd4
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDE29.out
files[2].path/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.out
files[2].size37769
files[2].sha256852f2bbb56cf982148f9f8a1420be3393375f60da44c91a9bfbe55090570dd65
files[2].rolepddf
files[3].statusdownloaded
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDE29.sascif
files[3].path/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.sascif
files[3].size1424933
files[3].sha25692c954a925b2d332e498b3bc725d95cc8839be6fbce8c9fe407e946540dedcf7
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDE29.zip
files[4].path/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.zip
files[4].size247207
files[4].sha256e9d0e694bb4aa2e6df051e3c17d3a5dc95bcb6f7c8f57453731079c552663cdb
files[4].rolefull_entry_zip
files[4].zip_member_count5
files[4].zip_validationcentral_directory_readable
View the complete summary.json source
{
  "code": "SASDE29",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.",
    "publication": {
      "title": "Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.",
      "author_list": "Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X",
      "journal": "Structure",
      "doi": "10.1016/j.str.2019.05.011",
      "pmid": "31257107",
      "published_date": "2019 Aug 6"
    },
    "status": "released",
    "submitted_date": "2018-12-11",
    "released_date": "2019-09-13"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDE29.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDE29.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE29_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE29_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE29_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE29_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE29.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Interleukin-1 receptor accessory protein ectodomain with RI linker",
          "short_name": null,
          "sequence": "SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVLEENKPTRPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKAAALHHILDAQKMVWNHRHHHHHH",
          "organism": "Homo sapiens",
          "uniprot_code": "Q9NPH3",
          "uniprot_range_first": 21,
          "uniprot_range_last": 348,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MTLLWCVVSLYFYGILQSDASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYST\nAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTT\nYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMG\nCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVVGSPKNA\nVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINE\nSISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQKVPAPRYTVEL\nACGFGATVLLVVILIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNAEEEEF\nVLLTLRGVLENEFGYKLCIFDRDSLPGGIVTDETLSFIQKSRRLLVVLSPNYVLQGTQAL\nLELKAGLENMASRGNINVILVQYKAVKETKVKELKRAKTVLTVIKWKGEKSKYPQGRFWK\nQLQVAMPVKKSPRRSSSDEQGLSYSSLKNV",
          "mw": 40.876,
          "total_mw": 40.876,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "10mM HEPES, 150mM NaCl, 3% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.2,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Interleukin-1 receptor accessory protein ectodomain with RI linker",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Taiwan",
            "full_name": "National Cheng Kung University",
            "webpage": "http://www.ncku.edu.tw/"
          }
        ],
        "contributor_name": "Shu-Ying",
        "contributor_surname": "Wang",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-08-11",
    "storage_temperature": null,
    "cell_temperature": 20.0,
    "exposure_time": 3.0,
    "number_of_frames": 600,
    "wavelength": 0.103,
    "sample_detector_distance": 1.5,
    "concentration_min": null,
    "concentration_max": 10.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.142,
    "s_max": 5.38,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDE29_fit1_model1_img.png",
          "software": "BILBOMD",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDE29_fit1_model1.pdb",
          "model_mw": 118.7,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 393
        },
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDE29_fit1_model2_img.png",
          "software": "BILBOMD",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDE29_fit1_model2.pdb",
          "model_mw": 118.7,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 393
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/multi_state_model_2_1_1_HTlPEKC_fitfoxs_img.png",
      "software": "MES-FoXS",
      "chi_square_value": 2.347585,
      "p_value": 0.0,
      "fit_residual_plot": "multi_state_model_2_1_1_HTlPEKC_fitfoxsresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDE29_fit1.dat",
      "fit_log": null,
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "4.5a",
  "i0_calibration_standard": null,
  "description": "SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.",
  "experiment_description": "SSynchrotron SAXS data from solutions of Interleukin-1 receptor accessory protein ectodomain with RI linker in 10mM HEPES, 150mM NaCl, 3% glycerol, pH 7.2 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected as 600 successive 3 second frames through the SEC elution profile. Sample-elution peak data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 49.8,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 82.55,
  "pddf_i0_error": null,
  "guinier_i0": 82.2,
  "guinier_i0_error": 0.86,
  "pddf_rg": 3.04,
  "pddf_rg_error": null,
  "guinier_rg": 3.037,
  "guinier_rg_error": 0.04,
  "pddf_dmax": 10.39,
  "pddf_dmax_error": null,
  "porod_volume": 76.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 48,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDE29_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-11-27T12:02:24.063588+01:00",
  "bragg_peak": []
}
View the complete manifest.json source
{
  "code": "SASDE29",
  "status": "success",
  "started_at": "2026-08-11T16:44:14.079839+00:00",
  "finished_at": "2026-08-11T16:44:23.629217+00:00",
  "source_last_modified": "2020-11-27T12:02:24.063588+01:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDE29/",
      "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/summary.json",
      "size": 8857,
      "sha256": "6481e3b9a0c1181133a43a154950c54ddb98c7827a51efdb018d66dd38b06224"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDE29.dat",
      "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.dat",
      "size": 36595,
      "sha256": "276e0483379834ffea97eb163b49414d6ef68fd5d6ca43770f038b0140d51dd4",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDE29.out",
      "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.out",
      "size": 37769,
      "sha256": "852f2bbb56cf982148f9f8a1420be3393375f60da44c91a9bfbe55090570dd65",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE29.sascif",
      "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.sascif",
      "size": 1424933,
      "sha256": "92c954a925b2d332e498b3bc725d95cc8839be6fbce8c9fe407e946540dedcf7",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDE29.zip",
      "path": "/data/saxsdb/sasbdb/entries/29/sasde29/source/SASDE29.zip",
      "size": 247207,
      "sha256": "e9d0e694bb4aa2e6df051e3c17d3a5dc95bcb6f7c8f57453731079c552663cdb",
      "role": "full_entry_zip",
      "zip_member_count": 5,
      "zip_validation": "central_directory_readable"
    }
  ]
}