C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism + Cu(II)

{
  "code": "SASDE38",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
    "publication": {
      "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
      "author_list": "Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G",
      "journal": "Biophysical Journal",
      "doi": "10.1016/j.bpj.2019.12.025",
      "pmid": null,
      "published_date": "2020 Jan"
    },
    "status": "released",
    "submitted_date": "2018-11-13",
    "released_date": "2019-11-25"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDE38.out",
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  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE38_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE38_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE38_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE38_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE38.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Major prion protein",
          "short_name": null,
          "sequence": "GQGGSHSQWNKPSKPKTNMKHVAGAAAAGAVVGGLGGYMLGSVMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITVKQHTVQTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGAS",
          "organism": "Ovis aries",
          "uniprot_code": "P23907",
          "uniprot_range_first": 94,
          "uniprot_range_last": 234,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MVKSHIGSWILVLFVAMWSDVGLCKKRPKPGGGWNTGGSRYPGQGSPGGNRYPPQGGGGW\nGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGGWGQGGSHSQWNKPSKPKTNMKHVAGAAA\nAGAVVGGLGGYMLGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDRYSNQNNFVH\nDCVNITVKQHTVTTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGASVILFSS\nPPVILLISFLIFLIVG",
          "mw": 16.085,
          "total_mw": 16.085,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 5.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism + Cu(II)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "ESRF",
            "address": "Grenoble, France",
            "full_name": "European Synchrotron Radiation Facility",
            "webpage": "http://www.esrf.eu/"
          }
        ],
        "contributor_name": "Gabriele",
        "contributor_surname": "Giachin",
        "orcid": "https://orcid.org/0000-0001-7550-0307"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-05-04",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 1,
    "wavelength": 0.099,
    "sample_detector_distance": 2.864,
    "concentration_min": null,
    "concentration_max": 7.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.103,
    "s_max": 4.928,
    "total_exposure_time": null,
    "seccolumn": null
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          "user": 388
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDE38_fit1_fixed_fit_img.png",
      "software": "EOM/GAJOE",
      "chi_square_value": 3.578,
      "p_value": 0.864685,
      "fit_residual_plot": "SASDE38_fit1_fitresiduals_img.png",
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      "software_version": null,
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of the C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism in 25 mM ammonium acetate, 250 mM NaCl, 0.001 mM CuSO4, pH 5.5 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column type, Superdex 200 5/150 GL (GE Healthcare); Flow rate: 0.15 mL/min; Total acquisition time: 25 min; Injection concentration: 7 mg/mL; Injection volume: 50 μL. The data obtained through the sample elution peak (865-905 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.",
  "tags": [],
  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
  "porod_mw": 15.6,
  "porod_mw_error": null,
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  "pddf_i0_error": null,
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  "guinier_i0_error": 0.03,
  "pddf_rg": 2.417,
  "pddf_rg_error": null,
  "guinier_rg": 2.15,
  "guinier_rg_error": 0.01,
  "pddf_dmax": 8.7,
  "pddf_dmax_error": null,
  "porod_volume": 28.8,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 57,
  "guinier_point_last": 113,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDE38_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-08-28T15:28:10.454623+02:00",
  "bragg_peak": []
}

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