Ribonuclease E from Escherichia coli

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  "status": "Published",
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    "title": "A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.",
    "publication": {
      "title": "A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.",
      "author_list": "Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ",
      "journal": "Sci Rep",
      "doi": "10.1038/s41598-019-44385-y",
      "pmid": "31138855",
      "published_date": "2019 May 28"
    },
    "status": "released",
    "submitted_date": "2018-07-30",
    "released_date": "2019-05-28"
  },
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        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": "1 x 5 mm",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Endoribonuclease E",
          "short_name": "RNase E",
          "sequence": "HHHHHHHHHHSSGHIEGRHMKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLELPEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAFRDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRLSPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASYLLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEEAMALPSEEEFAERKRPEQPAL",
          "organism": "Escherichia coli",
          "uniprot_code": "P21513",
          "uniprot_range_first": 1,
          "uniprot_range_last": 529,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVDY\nGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTFI\nSLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLELPEGMGLIVRTAGVGKSAEAL\nQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAFRDYLRQDIGEILIDNPKVLELA\nRQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDSTEALT\nAIDINSARATRGGDIEETAFNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQRAVE\nNRLREAVRQDRARIQISHISRFGLLEMSRQRLSPSLGESSHHVCPRCSGTGTVRDNESLS\nLSILRLIEEEALKENTQEVHAIVPVPIASYLLNEKRSAVNAIETRQDGVRCVIVPNDQME\nTPHYHVLRVRKGEETPTLSYMLPKLHEEAMALPSEEEFAERKRPEQPALATFAMPDVPPA\nPTPAEPAAPVVAPAPKAAPATPAAPAQPGLLSRFFGALKALFSGGEETKPTEQPAPKAEA\nKPERQQDRRKPRQNNRRDRNERRDTRSERTEGSDNREENRRNRRQAQQQTAETRESRQQA\nEVTEKARTADEQQAPRRERSRRRNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRR\nKQRQLNQKVRYEQSVAEEAVVAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQ\nQEENNADNRDNGGMPRRSRRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASG\nKVWIRYPIVRPQDVQVEEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPV\nQVAEPQPEVVETTHPEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEV\nVETAEVVVAEPEVVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVP\nEAPRHSDWQRPTFAFEGKGAAGGHTATHHASAAPARPQPVE",
          "mw": 61.84,
          "total_mw": 247.36,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris",
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        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ribonuclease E from Escherichia coli",
      "ext_coefficient": null,
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      "absorbption": null,
      "deuteration": null,
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    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Portsmouth, UK",
            "full_name": "University of Portsmouth",
            "webpage": "https://www.port.ac.uk"
          }
        ],
        "contributor_name": "A",
        "contributor_surname": "Callaghan",
        "orcid": ""
      }
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    "date": "2017-02-11",
    "storage_temperature": 21.0,
    "cell_temperature": 15.0,
    "exposure_time": 3.0,
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    "wavelength": 0.1,
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          "bead_radius": 5.0,
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          "user": 338
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      ],
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  "experiment_description": "Synchrotron SAXS data from solutions of Ribonuclease E from Escherichia coli in 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH 8 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.58 mg/ml was measured at 15°C. 640 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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