SASDE63

Labeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00

The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).

▼

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Labeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant

浓度— – 2.0 缓冲液 / pHPBS, 10 mM DTT, 6 M urea, 0.3 M KCl / 7.4
实验温度23.0 设备 / 束线PETRA III / EMBL P12
波长0.1 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
查看序列
>U=P-ACETYLPHENYLALANINE.CWASLABELEDWITHALEXAFLUOR594C5MALEIMIDE.UWASLABELEDWITHALEXAFLUOR488C5HYDROXYLAMINEACSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAUA
proteinEscherichia coliP0AFG6115–151monomer分子数 14.348 kDa
Alexa Fluor™ 594 C5 Maleimideother———–—monomer分子数 10.886 kDa
Alexa Fluor™ 488 C5 Hydroxylamineother———–—monomer分子数 10.7 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11694[3]0.0290642–4.48844 1/nm含误差列缺失 01sasbdb/entries/63/sasde63/source/SASDE63.dat
▼

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.73—nm
i0Guinier1672.58.3arbitrary
i0P(r)1706.016.4arbitrary
mwExperimental7.3—kDa
porod_volumePorod13.09—nm³
rgGuinier2.30.4nm
rgP(r)2.6280.06nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

▼

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

▶

4. 来源文件索引 Source Files

▶

5. 实验说明与论文 Experiment & Publication

▼

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称PBS, 10 mM DTT, 6 M urea, 0.3 M KCl缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-11-08储存 / 测量温度— / 23.0
曝光时间0.05帧数20
波长0.1样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.029 – 4.488样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/63/sasde63/source/SASDE63.datdownloaded765257c0cef14f0b84e906a9703ae77da4cbea39381f1ef823ff5515f78a784ee472f下载查看原文件源站
full_entry_zipsasbdb/entries/63/sasde63/source/SASDE63.zipdownloaded421372dab3c2c977137715d479daf55205ef20cd7b394376b137a8399e6029c31f653下载查看原文件源站
pddfsasbdb/entries/63/sasde63/source/SASDE63.outdownloaded8285441c58dc6d6933ec1d7165f3edfe9b862c33eb30f97fbaa98a946eeea9329a7dc下载查看原文件源站
sascifsasbdb/entries/63/sasde63/source/SASDE63.sascifdownloaded15051196e27878b11820b934169219d9d596a22107f6e6a87fad6eecdb515f261ff56c下载查看原文件源站
summarysasbdb/entries/63/sasde63/source/summary.jsondownloaded77137030e85e6f8e92cb978434056986259614087f4ef6f672e46ae228e13dfb4af8下载查看原文件源站
curve:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.dat",
  "role": "curve",
  "sha256": "7c0cef14f0b84e906a9703ae77da4cbea39381f1ef823ff5515f78a784ee472f",
  "size": 76525,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDE63.dat"
}
full_entry_zip:来源记录与 ZIP 内部目录(2 项)
{
  "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.zip",
  "role": "full_entry_zip",
  "sha256": "2dab3c2c977137715d479daf55205ef20cd7b394376b137a8399e6029c31f653",
  "size": 42137,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDE63.zip",
  "zip_member_count": 2,
  "zip_members": [
    {
      "crc32": "98bec74e",
      "name": "SASDE63/experimental_data/SASDE63.dat",
      "size": 76525
    },
    {
      "crc32": "7917fb01",
      "name": "SASDE63/pddf/SASDE63.out",
      "size": 82854
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.out",
  "role": "pddf",
  "sha256": "41c58dc6d6933ec1d7165f3edfe9b862c33eb30f97fbaa98a946eeea9329a7dc",
  "size": 82854,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDE63.out"
}
sascif:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.sascif",
  "role": "sascif",
  "sha256": "96e27878b11820b934169219d9d596a22107f6e6a87fad6eecdb515f261ff56c",
  "size": 150511,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE63.sascif"
}
summary:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/summary.json",
  "sha256": "7030e85e6f8e92cb978434056986259614087f4ef6f672e46ae228e13dfb4af8",
  "size": 7713,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDE63/"
}

全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:168 个字段值
字段路径原始值
codeSASDE63
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleDecoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
project.publication.titleDecoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
project.publication.author_listFuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
project.publication.journalProc Natl Acad Sci U S A
project.publication.doi10.1073/pnas.1704692114
project.publication.pmid28716919
project.publication.published_date2017 Aug 1
project.statusreleased
project.submitted_date2018-08-16
project.released_date2018-08-18
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDE63.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDE63.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE63_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE63_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE63_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE63_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDE63.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
experiment.sample.molecule[0].short_nameBBL-488/594
experiment.sample.molecule[0].sequence> U=p-acetylphenylalanine. C was labeled with Alexa Fluor 594 C5 maleimide. U was labeled with Alexa Fluor 488 C5 hydroxylamine ACSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAUA
experiment.sample.molecule[0].organismEscherichia coli
experiment.sample.molecule[0].uniprot_codeP0AFG6
experiment.sample.molecule[0].uniprot_range_first115
experiment.sample.molecule[0].uniprot_range_last151
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV LEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR LLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVP MTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIK ELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNG QVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV
experiment.sample.molecule[0].mw4.348
experiment.sample.molecule[0].total_mw4.348
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameAlexa Fluor™ 594 C5 Maleimide
experiment.sample.molecule[1].short_nameAlexa594
experiment.sample.molecule[1].sequencenull
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeother
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw0.886
experiment.sample.molecule[1].total_mw0.886
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_statenull
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.molecule[2].long_nameAlexa Fluor™ 488 C5 Hydroxylamine
experiment.sample.molecule[2].short_nameAlexa488
experiment.sample.molecule[2].sequencenull
experiment.sample.molecule[2].organismnull
experiment.sample.molecule[2].uniprot_codenull
experiment.sample.molecule[2].uniprot_range_firstnull
experiment.sample.molecule[2].uniprot_range_lastnull
experiment.sample.molecule[2].oligomerizationmonomer
experiment.sample.molecule[2].molecular_typeother
experiment.sample.molecule[2].uniprot_sequencenull
experiment.sample.molecule[2].mw0.7
experiment.sample.molecule[2].total_mw0.7
experiment.sample.molecule[2].number_molecules1
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_descriptionnull
experiment.sample.buffer.namePBS, 10 mM DTT, 6 M urea, 0.3 M KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLabeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressPrague, Czech Republic
experiment.contributor[0].affiliation[0].full_nameInstitute of Biotechnology CAS v.v.i.
experiment.contributor[0].affiliation[0].webpagehttp://www.ibt.cas.cz/
experiment.contributor[0].contributor_nameGustavo
experiment.contributor[0].contributor_surnameFuertes Vives
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-8564-8644
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-11-08
experiment.storage_temperaturenull
experiment.cell_temperature23.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.1
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max2.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.029
experiment.s_max4.488
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).
experiment_descriptionnull
tags[0]idp
intensity_unitarbitrary
experimental_mw7.3
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i01706.0
pddf_i0_error16.4
guinier_i01672.5
guinier_i0_error8.3
pddf_rg2.628
pddf_rg_error0.06
guinier_rg2.3
guinier_rg_error0.4
pddf_dmax10.73
pddf_dmax_errornull
porod_volume13.09
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first21
guinier_point_last201
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDE63_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDE63
statussuccess
started_at2026-08-11T13:53:17.293541+00:00
finished_at2026-08-11T13:53:25.641028+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDE63/
files[0].path/data/saxsdb/sasbdb/entries/63/sasde63/source/summary.json
files[0].size7713
files[0].sha2567030e85e6f8e92cb978434056986259614087f4ef6f672e46ae228e13dfb4af8
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDE63.dat
files[1].path/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.dat
files[1].size76525
files[1].sha2567c0cef14f0b84e906a9703ae77da4cbea39381f1ef823ff5515f78a784ee472f
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDE63.out
files[2].path/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.out
files[2].size82854
files[2].sha25641c58dc6d6933ec1d7165f3edfe9b862c33eb30f97fbaa98a946eeea9329a7dc
files[2].rolepddf
files[3].statusdownloaded
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDE63.sascif
files[3].path/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.sascif
files[3].size150511
files[3].sha25696e27878b11820b934169219d9d596a22107f6e6a87fad6eecdb515f261ff56c
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDE63.zip
files[4].path/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.zip
files[4].size42137
files[4].sha2562dab3c2c977137715d479daf55205ef20cd7b394376b137a8399e6029c31f653
files[4].rolefull_entry_zip
files[4].zip_member_count2
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDE63",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.",
    "publication": {
      "title": "Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.",
      "author_list": "Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA",
      "journal": "Proc Natl Acad Sci U S A",
      "doi": "10.1073/pnas.1704692114",
      "pmid": "28716919",
      "published_date": "2017 Aug 1"
    },
    "status": "released",
    "submitted_date": "2018-08-16",
    "released_date": "2018-08-18"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDE63.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDE63.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE63_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE63_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE63_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE63_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE63.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex",
          "short_name": "BBL-488/594",
          "sequence": "> U=p-acetylphenylalanine. C was labeled with Alexa Fluor 594 C5 maleimide. U was labeled with Alexa Fluor 488 C5 hydroxylamine\r\nACSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAUA",
          "organism": "Escherichia coli",
          "uniprot_code": "P0AFG6",
          "uniprot_range_first": 115,
          "uniprot_range_last": 151,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV\nLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR\nLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVP\nMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK\nAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIK\nELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNG\nQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV",
          "mw": 4.348,
          "total_mw": 4.348,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Alexa Fluor™ 594 C5 Maleimide",
          "short_name": "Alexa594",
          "sequence": null,
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 0.886,
          "total_mw": 0.886,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Alexa Fluor™ 488 C5 Hydroxylamine",
          "short_name": "Alexa488",
          "sequence": null,
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 0.7,
          "total_mw": 0.7,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "PBS, 10 mM DTT, 6 M urea, 0.3 M KCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Labeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Prague, Czech Republic",
            "full_name": "Institute of Biotechnology CAS v.v.i.",
            "webpage": "http://www.ibt.cas.cz/"
          }
        ],
        "contributor_name": "Gustavo",
        "contributor_surname": "Fuertes Vives",
        "orcid": "https://orcid.org/0000-0002-8564-8644"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2013-11-08",
    "storage_temperature": null,
    "cell_temperature": 23.0,
    "exposure_time": 0.05,
    "number_of_frames": 20,
    "wavelength": 0.1,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
    "concentration_max": 2.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.029,
    "s_max": 4.488,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).",
  "experiment_description": null,
  "tags": [
    "idp"
  ],
  "intensity_unit": "arbitrary",
  "experimental_mw": 7.3,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 1706.0,
  "pddf_i0_error": 16.4,
  "guinier_i0": 1672.5,
  "guinier_i0_error": 8.3,
  "pddf_rg": 2.628,
  "pddf_rg_error": 0.06,
  "guinier_rg": 2.3,
  "guinier_rg_error": 0.4,
  "pddf_dmax": 10.73,
  "pddf_dmax_error": null,
  "porod_volume": 13.09,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 21,
  "guinier_point_last": 201,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDE63_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDE63",
  "status": "success",
  "started_at": "2026-08-11T13:53:17.293541+00:00",
  "finished_at": "2026-08-11T13:53:25.641028+00:00",
  "source_last_modified": "2023-06-21T07:56:39.492710+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDE63/",
      "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/summary.json",
      "size": 7713,
      "sha256": "7030e85e6f8e92cb978434056986259614087f4ef6f672e46ae228e13dfb4af8"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDE63.dat",
      "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.dat",
      "size": 76525,
      "sha256": "7c0cef14f0b84e906a9703ae77da4cbea39381f1ef823ff5515f78a784ee472f",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDE63.out",
      "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.out",
      "size": 82854,
      "sha256": "41c58dc6d6933ec1d7165f3edfe9b862c33eb30f97fbaa98a946eeea9329a7dc",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE63.sascif",
      "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.sascif",
      "size": 150511,
      "sha256": "96e27878b11820b934169219d9d596a22107f6e6a87fad6eecdb515f261ff56c",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDE63.zip",
      "path": "/data/saxsdb/sasbdb/entries/63/sasde63/source/SASDE63.zip",
      "size": 42137,
      "sha256": "2dab3c2c977137715d479daf55205ef20cd7b394376b137a8399e6029c31f653",
      "role": "full_entry_zip",
      "zip_member_count": 2,
      "zip_validation": "central_directory_readable"
    }
  ]
}