C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A

{
  "code": "SASDE86",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle.",
    "publication": {
      "title": "Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle.",
      "author_list": "Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K",
      "journal": "Nature",
      "doi": "10.1038/s41586-019-1095-5",
      "pmid": "30944476",
      "published_date": "2019 Apr"
    },
    "status": "released",
    "submitted_date": "2018-10-10",
    "released_date": "2019-04-02"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDE86.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDE86.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE86_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE86_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE86_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE86_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE86.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "ATP-citrate lyase",
          "short_name": "ACL",
          "sequence": "AKILEGPAMKLFNKWGIPVPNYVVIMDPDRLAQLGEANKWLRESKLVVKAHEAIGGRFKL\r\n GLVKIGLNLEQAIEAAKEMLGAKVGTAEIGQVIIAEMLEHDAEFYVSIIGNKDGAELLIS\r\n KHGGVDIEDNWDSVRRIQIELDENPTIEQLTELAKDAGFEGEIAERVGKICSRLILCFDN\r\n EDAQSIEINPLVIRKSDMRFAALDAVMNVDYDARFRHADWDFKPVSEIGRPFTEAEQQIM\r\n EIDSRIKGSVKFVEVPGGEIALLTAGGGASVFYADAVVARGGTIANYAEYSGDPADWAVE\r\n ALTETICRLPNIKHIIVGGAIANFTDVKATFSGIINGFRESKSKGYLEGVKIWVRRGGPN\r\n EAQGLAAIKQLQEEGFDIHVYDRSMPMTDIVDLAMKSSILANKDTRAVIIGGVAGVNAAK\r\n RMAQFDFLVNRPLTVQAFVYPPEAGQQKEIFRGGELKNVTVYDSLAPALEEHPDINTALI\r\n YLGASRAAQAAKEALESPNIQLVSMITEGVPEKDAKRLKKLAQKLGKMLNGPSSIGIMSA\r\n GECRLGVIGGEFKNLKLCNLYRQGSFGVLTKSGGLSNEAMWLCAQNGDGITSAVAIGGDA\r\n YPGTDFVTYLEMFEKDPATKAVVMIGEVGGNLEEEAAEWLAAEPRRIKLIAAIGGTCQEV\r\n LKGAGSARSKMNALRDAGAYVPDTFGGLSKEIKKVYEELIAAGEISTEIDEAVLPELPPR\r\n VQEVMKQGEVIVEPLIRTTISDDRGEEPRYAGYAASELCSKGYGIEDVIGLLWNKKLPTR\r\n EESEIIKRIVMISADHGPAVSGAFGSILAACAGIDMPQAVSAGMTMIGPRFGGAVTNAGK\r\n YFKMAVEDYPNDIPGFLSWMKKNVGPVPGIGHRVKSVKNPDQRVKYLVSYIKNETSLHTP\r\n CLDYALEVEKVTTAKKGNLILNVDGTIGCILMDLDFPVHSLNGFFVLARTIGMIGHWIDQ\r\n NNQNSRLIRLYDYLINYAVKPEQEVPEK",
          "organism": "Chlorobium limicola",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
          "mw": 107.314,
          "total_mw": 429.256,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.2,
        "deuteration": null
      },
      "purity_method": null,
      "name": "C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
        ],
        "contributor_name": "Clement",
        "contributor_surname": "Blanchet",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-09-04",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 900,
    "wavelength": 0.124,
    "sample_detector_distance": 3.1,
    "concentration_min": null,
    "concentration_max": 16.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.025,
    "s_max": 5.064,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of C. limicola acetyl citrate lyase ACL in 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM coenzyme-A, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Size-exclusion chromatography SAXS (SEC-SAXS) was employed using a sample injection concentration of 16.00 mg/ml. Data were measured at through the SEC elution at 20°C. 900 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank was subtracted using the CHROMIXS SEC-SAXS analysis package.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 420.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 494.0,
  "porod_mw_error": null,
  "pddf_i0": 111400.0,
  "pddf_i0_error": null,
  "guinier_i0": 110407.0,
  "guinier_i0_error": 61.0,
  "pddf_rg": 5.8,
  "pddf_rg_error": null,
  "guinier_rg": 5.69,
  "guinier_rg_error": 0.03,
  "pddf_dmax": 17.5,
  "pddf_dmax_error": null,
  "porod_volume": 791.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 13,
  "guinier_point_last": 54,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDE86_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
  "bragg_peak": []
}

{
  "code": "SASDE86",
  "status": "success",
  "started_at": "2026-08-11T15:03:45.296186+00:00",
  "finished_at": "2026-08-11T15:03:53.675989+00:00",
  "source_last_modified": "2023-06-21T07:56:39.492710+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDE86/",
      "path": "/data/saxsdb/sasbdb/entries/86/sasde86/source/summary.json",
      "size": 7270,
      "sha256": "63253b43731359e1de3fda9280182d5596706f42f79e5c460fbed8a2d7a28223"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDE86.dat",
      "path": "/data/saxsdb/sasbdb/entries/86/sasde86/source/SASDE86.dat",
      "size": 85275,
      "sha256": "6b11a8a9d66f535f415822f79238f44783e90c220b5b22d66da7c18634d44435",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDE86.out",
      "path": "/data/saxsdb/sasbdb/entries/86/sasde86/source/SASDE86.out",
      "size": 48481,
      "sha256": "72fa086a1b156d274f0bf17c5b9894d3722e10461ae1909f60805e3c521791a9",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDE86.sascif",
      "path": "/data/saxsdb/sasbdb/entries/86/sasde86/source/SASDE86.sascif",
      "size": 137279,
      "sha256": "474b7aa78cbaa4fb997197aaf7bb58099986ad4dc9570459296138a8c2526828",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDE86.zip",
      "path": "/data/saxsdb/sasbdb/entries/86/sasde86/source/SASDE86.zip",
      "size": 36813,
      "sha256": "05c8147deb5d7642e16b080949b4ced8bf4b0bfdb5fa56ebcc96ee2232093669",
      "role": "full_entry_zip",
      "zip_member_count": 2,
      "zip_validation": "central_directory_readable"
    }
  ]
}