查看完整 summary.json 原文
{
"code": "SASDEG2",
"status": "Published",
"type_of_curve": "Other",
"angular_unit": "1/nm",
"project": {
"title": "Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.",
"publication": {
"title": "Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.",
"author_list": "Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P",
"journal": "Cell",
"doi": "10.1016/j.cell.2018.10.039",
"pmid": "30445040",
"published_date": "2018 Nov 15"
},
"status": "released",
"submitted_date": "2018-08-13",
"released_date": "2018-11-21"
},
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},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
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"long_name": "Mitochondrial import inner membrane translocase subunit TIM9",
"short_name": "TIM9",
"sequence": "MDALNSKEQQEFQKVVEQKQMKDFMRLYSNLVERCFTDCVNDFTTSKLTNKEQTCIMKCSEKFLKHSE RVGQRFQEQNAALGQGLGR",
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"uniprot_code": "O74700",
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},
{
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},
{
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"molecular_type": "protein",
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}
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"pka": null,
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"deuteration": null
},
"purity_method": null,
"name": "Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))",
"ext_coefficient": null,
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},
"contributor": [
{
"affiliation": [
{
"short_name": "EMBL",
"address": "Grenoble, France",
"full_name": "European Molecular Biology Laboratory (EMBL) - Grenoble Outstation",
"webpage": "https://www.embl.fr/"
}
],
"contributor_name": "Martha",
"contributor_surname": "Brennich",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2016-02-22",
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"cell_temperature": 20.0,
"exposure_time": 1.0,
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"experiment_description": "Synchrotron SAXS data from solutions of the mitochondrial import inner membrane translocase complex (TIM9·10) in complex with a precursor (GDP/GTP carrier (Ggc1)) in 50mM Tris, 150mM NaCl, imidiazole, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data was collected directly on the eluent from an Ni-NTA affinity chromatography column to ensure the isolation of a stable, non-aggregated complex. A solute concentration of approximately 2.00 mg/ml was measured at 20°C. 50 successive 1 second frames were collected through the elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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"last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
