SASDEG6

Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2022-03-31T21:55:43.237981+02:00

SEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 4.6 mg/ml.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)

浓度— – 4.6 缓冲液 / pH20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP / 7.5
实验温度25.0 设备 / 束线Diamond Light Source / B21
波长0.1 nm曝光3.0 s × 100

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Epstein-Barr nuclear antigen 2
查看序列
>EBNA2_TYPE2_348-422SMPQLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDSEEPPFLFPSDWYPPTLEPAELD
proteinHuman gammaherpesvirus 4Q69022348–422dimer分子数 27.934 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1913[4]0.0340534–0.244221 1/A含误差列缺失 00sasbdb/entries/g6/sasdeg6/source/SASDEG6.dat
▼

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.35—Å
i0Guinier0.0268127.111e-05—
i0P(r)0.02772——
mwExperimental18.1—kDa
porod_volumePorod20.0—ų
rgGuinier2.770.09Å
rgP(r)3.01—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-09-23储存 / 测量温度4.0 / 25.0
曝光时间3.0帧数100
波长0.1样品-探测器距离4.014
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.341 – 2.442样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/g6/sasdeg6/source/SASDEG6.datdownloaded4662255a5bac79d161969b8ee85643f97bb894ad2e0d231d49d21efbf8333e58402ee下载查看原文件源站
full_entry_zipsasbdb/entries/g6/sasdeg6/source/SASDEG6.zipdownloaded579928d9700ccf95a4c39221941d41af6cd81416b0a2aefd597ca12bf828a93a870c1下载查看原文件源站
pddfsasbdb/entries/g6/sasdeg6/source/SASDEG6.outdownloaded832574aca477280ad3b5c2ca977e07cde4aa90dbcb109e38490f158f1961833672ee7下载查看原文件源站
sascifsasbdb/entries/g6/sasdeg6/source/SASDEG6.sascifdownloaded214565457cf25d58ab751cf4266135d24bef4375b76837b5d0150a876c89f62843e938下载查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(5 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:169 个字段值
字段路径原始值
codeSASDEG6
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleIncreased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth
project.publication.titleIncreased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth
project.publication.author_listPonnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
project.publication.journalnull
project.publication.doi10.1101/464131
project.publication.pmidnull
project.publication.published_date2018 Nov 06
project.statusreleased
project.submitted_date2018-10-16
project.released_date2019-06-19
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometry1 x 5 mm
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEpstein-Barr nuclear antigen 2
experiment.sample.molecule[0].short_nameEBNA2 Type2
experiment.sample.molecule[0].sequence>EBNA2_Type2_348-422 SMPQLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDSEEPPFLFPSDWYPPTLEPAELD
experiment.sample.molecule[0].organismHuman gammaherpesvirus 4
experiment.sample.molecule[0].uniprot_codeQ69022
experiment.sample.molecule[0].uniprot_range_first348
experiment.sample.molecule[0].uniprot_range_last422
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPTYYLALHGGQSYNLIVDTDMSGNPSLSVIPTNPYQEQLSNNPLIQLQIVVGENTGAPA PPQPPPPPPPPPPPERRDAWTQEPLPLDMNPLGSDASQGPLASSIRMLCMAQYLLRNARG QQGLLRPLGPQTRSQVTLERQPVHNPRQEAPIILLQSPAPPRFTPVPMVALGHTLQPTPP PRPTLPQPRIPLIIPPRHTNQPATTPPTAPQRLTLGHQLSLPPHPPPHQSTPHCSSDSTG LPPPPTSYSIPSMTLSPEPLPPPAAPAHPLPGVIYDQQALPPTPGPPWWPPVRDPTPTTQ TPPTNTKQGPDQGQGRGRWRGRGRSKGRGRMHKLPEPRRPGPDTSSPSMPQLSPVVSLHQ GQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDSEEPPFLFPSDWYPPTLEPAE LDESWEGIFETTESHSSDEENVGGPSKRPRTSTQ
experiment.sample.molecule[0].mw7.934
experiment.sample.molecule[0].total_mw15.868
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEpstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressBrighton, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Sussex
experiment.contributor[0].affiliation[0].webpagehttp://www.sussex.ac.uk/
experiment.contributor[0].contributor_nameRajesh
experiment.contributor[0].contributor_surnamePonnusamy
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-09-23
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time3.0
experiment.number_of_frames100
experiment.wavelength0.1
experiment.sample_detector_distance4.014
experiment.concentration_minnull
experiment.concentration_max4.6
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.341
experiment.s_max2.442
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwareDAMMIN
fits[0].chi_square_value1.783
fits[0].p_value0.591803
fits[0].fit_residual_plotSASDEG6_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 4.6 mg/ml.
experiment_descriptionSynchrotron SAXS data from solutions of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) in 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The sample was measured using size exclusion chromatography SAXS (SEC-SAXS) at 25°C. 100 successive 3 second frames were collected through the SEC sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted.
tags[0]idp
intensity_unitnull
experimental_mw18.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.02772
pddf_i0_errornull
guinier_i00.026812
guinier_i0_error7.111e-05
pddf_rg3.01
pddf_rg_errornull
guinier_rg2.77
guinier_rg_error0.09
pddf_dmax10.35
pddf_dmax_errornull
porod_volume20.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last59
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEG6_datloglog_img.png
symmetrynull
last_modified2022-03-31T21:55:43.237981+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEG6
statussuccess
started_at2026-08-11T15:09:38.333144+00:00
finished_at2026-08-11T15:09:49.721366+00:00
source_last_modified2022-03-31T21:55:43.237981+02:00
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查看完整 summary.json 原文
{
  "code": "SASDEG6",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth",
    "publication": {
      "title": "Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth",
      "author_list": "Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M",
      "journal": null,
      "doi": "10.1101/464131",
      "pmid": null,
      "published_date": "2018 Nov 06"
    },
    "status": "released",
    "submitted_date": "2018-10-16",
    "released_date": "2019-06-19"
  },
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        "resolution": null
      },
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      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": "1 x 5 mm",
      "type_of_source": "X-ray synchrotron",
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Epstein-Barr nuclear antigen 2",
          "short_name": "EBNA2 Type2",
          "sequence": ">EBNA2_Type2_348-422\r\nSMPQLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDSEEPPFLFPSDWYPPTLEPAELD",
          "organism": "Human gammaherpesvirus 4",
          "uniprot_code": "Q69022",
          "uniprot_range_first": 348,
          "uniprot_range_last": 422,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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        "comment": null,
        "additive": null,
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      "name": "Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)",
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        "affiliation": [
          {
            "short_name": null,
            "address": "Brighton, UK",
            "full_name": "University of Sussex",
            "webpage": "http://www.sussex.ac.uk/"
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        "contributor_name": "Rajesh",
        "contributor_surname": "Ponnusamy",
        "orcid": ""
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-09-23",
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      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEG6_fit1_model1_img.png",
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}
查看完整 manifest.json 原文
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