SASDEJ3

Truncated neutophil cytosol factor 1, p47phox [1-342]

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Truncated neutophil cytosol factor 1, p47phox [1-342]

浓度0.9 – 3.6 缓冲液 / pH50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol / 7.5
实验温度6.0 设备 / 束线IBBMC / Bruker Nanostar
波长0.154 nm曝光3600.0 s × 11

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Neutophil cytosol factor 1
查看序列
GPLGSPNSARMGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIADYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPG
proteinHomo sapiensP145981–342monomer分子数 140.398 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1309[3]0.014225–0.452118 1/A含误差列缺失 00sasbdb/entries/j3/sasdej3/source/SASDEJ3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.00.5Å
i0Guinier0.02870.00031/cm
i0P(r)0.028360.00031/cm
mwExperimental39.4—kDa
mwGuinier I(0)38.04.0kDa
mwPorod38.5—kDa
porod_volumePorod57.8—ų
rgGuinier2.640.06Å
rgP(r)2.630.06Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

拟合 1 · BUNCH 08

χ²:0.971CorMap p:0.419653单位:1/A

p47phoxDCter in solution: Comparison of the experimental data (blue dots) with the calculated scattering pattern (red line) of the BUNCH model shown on the right chi2=0.97. The ultimate adjustment shown here was performed using the program CRYSOL.

模型名称 / 类型软件对称性模型 MWPDB 声明来源文件
1—atomicOther —40.4—模型 图片
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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2009-10-16储存 / 测量温度6.0 / 6.0
曝光时间3600.0帧数11
波长0.154样品-探测器距离0.662
光源X-ray in house探测器VÅNTEC-2000
机构 / 束线IBBMC / Bruker Nanostar · Orsay, France
q 范围0.142 – 4.521样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/j3/sasdej3/source/SASDEJ3.outdownloaded2613715eaa006f7e77afca2579d0a80ce347bdd3b106d7a595aa52dd3a6c92ed7e9e0下载查看原文件源站
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summarysasbdb/entries/j3/sasdej3/source/summary.jsondownloaded856006aed9b1b796e93366173fa3aea9daef628275f0fdf2c3187df158062c51ae90下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDEJ3
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/A
project.titleQuantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.
project.publication.titleQuantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.
project.publication.author_listZiegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.RA118.006864
project.publication.pmid30630949
project.publication.published_date2019 Jan 10
project.statusreleased
project.submitted_date2018-08-28
project.released_date2019-01-16
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experiment.instrument.detector.typeMikroGap gas detector
experiment.instrument.detector.nameVÅNTEC-2000
experiment.instrument.detector.resolution200.0
experiment.instrument.nameIBBMC
experiment.instrument.cityOrsay
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBruker Nanostar
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNeutophil cytosol factor 1
experiment.sample.molecule[0].short_namep47phox
experiment.sample.molecule[0].sequenceGPLGSPNSARMGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEF HKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISR CPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIADYEKT SGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEP YVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQA QRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPG
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP14598
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last342
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPI EAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKV RPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTG DVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAV EGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPP RRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSK PQPAVPPRPSADLILNRCSESTKRKLASAV
experiment.sample.molecule[0].mw40.398
experiment.sample.molecule[0].total_mw40.398
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTruncated neutophil cytosol factor 1, p47phox [1-342]
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressParis, France
experiment.contributor[0].affiliation[0].full_nameI2BC-CNRS
experiment.contributor[0].affiliation[0].webpagehttp://www.i2bc.paris-saclay.fr
experiment.contributor[0].contributor_nameDominique
experiment.contributor[0].contributor_surnameDURAND
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2009-10-16
experiment.storage_temperature6.0
experiment.cell_temperature6.0
experiment.exposure_time3600.0
experiment.number_of_frames11
experiment.wavelength0.154
experiment.sample_detector_distance0.662
experiment.concentration_min0.9
experiment.concentration_max3.6
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.142
experiment.s_max4.521
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDEJ3_fit1_model1_img.png
fits[0].models[0].softwareOther
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
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fits[0].models[0].commentnull
fits[0].models[0].user262
fits[0].fit_unit1/A
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fits[0].softwareBUNCH
fits[0].chi_square_value0.971
fits[0].p_value0.419653
fits[0].fit_residual_plotfit4columns1676_fitresiduals_img.png
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fits[0].software_version08
fits[0].descriptionp47phoxDCter in solution: Comparison of the experimental data (blue dots) with the calculated scattering pattern (red line) of the BUNCH model shown on the right chi2=0.97. The ultimate adjustment shown here was performed using the program CRYSOL.
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSAXS data from solutions of p47phox [1-342] in 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH 7.5, were collected on a Bruker Nanostar instrument at the IBBMC (Orsay, France) using a MikroGap VÅNTEC-2000 detector at a sample-detector distance of 0.7 m and at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Samples of were studied at different concentrations ranging from 0.9 to 3.6 mg/ml measured at 6°C. 11 successive 3600 second frames were collected. SAXS data were normalized to the intensity of the incident beam, averaged and background subtracted using the program package PRIMUS. Intensities were put on an absolute scale (cm-1) using water scattering. The final pattern used for fitting was obtained by extrapolating to infinite dilution of the set of curves recorded at the different concentrations. Molecular mass was obtained using the bayesian inference approach proposed in PRIMUS/qt, which combines four concentration independent MM estimators: M=39.4 kDa within the credibility interval [36.5-39.8].
tags[]
intensity_unit1/cm
experimental_mw39.4
experimental_mw_errornull
guinier_i0_mw38.0
guinier_i0_mw_error4.0
porod_mw38.5
porod_mw_errornull
pddf_i00.02836
pddf_i0_error0.0003
guinier_i00.0287
guinier_i0_error0.0003
pddf_rg2.63
pddf_rg_error0.06
guinier_rg2.64
guinier_rg_error0.06
pddf_dmax10.0
pddf_dmax_error0.5
porod_volume57.8
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last23
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEJ3_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEJ3
statussuccess
started_at2026-08-11T14:01:16.185814+00:00
finished_at2026-08-11T14:01:24.786857+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDEJ3",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/A",
  "project": {
    "title": "Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.",
    "publication": {
      "title": "Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.",
      "author_list": "Ziegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.RA118.006864",
      "pmid": "30630949",
      "published_date": "2019 Jan 10"
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          "organism": "Homo sapiens",
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          "uniprot_range_last": 342,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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        "concentration_unit": null,
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        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
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      "name": "Truncated neutophil cytosol factor 1, p47phox  [1-342]",
      "ext_coefficient": null,
      "contrast": null,
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      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
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    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
            "address": "Paris, France",
            "full_name": "I2BC-CNRS",
            "webpage": "http://www.i2bc.paris-saclay.fr"
          }
        ],
        "contributor_name": "Dominique",
        "contributor_surname": "DURAND",
        "orcid": null
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2009-10-16",
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    "wavelength": 0.154,
    "sample_detector_distance": 0.662,
    "concentration_min": 0.9,
    "concentration_max": 3.6,
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    "s_min": 0.142,
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          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEJ3_fit1_model1_img.png",
          "software": "Other",
          "pdb_link": [],
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          "type_of_model": "atomic",
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      "software_version": "08",
      "description": "p47phoxDCter  in solution: \r\nComparison of the experimental data (blue dots) with the calculated scattering pattern (red line) of the BUNCH model shown on the right chi2=0.97. The ultimate adjustment shown here was performed using the program CRYSOL."
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  "experiment_description": "SAXS data from solutions of p47phox [1-342] in 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH 7.5, were collected on a Bruker Nanostar instrument at the IBBMC (Orsay, France) using a MikroGap VÅNTEC-2000 detector at a sample-detector distance of 0.7 m and at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Samples of were studied at different concentrations ranging from 0.9 to 3.6 mg/ml measured at 6°C. 11 successive 3600 second frames were collected. SAXS data were normalized to the intensity of the incident beam, averaged and background subtracted using the program package PRIMUS. Intensities were put on an absolute scale (cm-1) using water scattering. The final pattern used for fitting was obtained by extrapolating to infinite dilution of the set of curves recorded at the different concentrations. Molecular mass was obtained using the bayesian inference approach proposed in PRIMUS/qt, which combines four concentration independent MM estimators: M=39.4 kDa within the credibility interval [36.5-39.8].",
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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