Dimeric class D beta-lactamase OXA-48

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  "project": {
    "title": "The biological assembly of OXA-48 reveals a dimer interface with high charge complementarity and very high affinity.",
    "publication": {
      "title": "The biological assembly of OXA-48 reveals a dimer interface with high charge complementarity and very high affinity.",
      "author_list": "Lund BA, Thomassen AM, Nesheim BHB, Carlsen TJO, Isaksson J, Christopeit T, Leiros HS",
      "journal": "FEBS J",
      "doi": "10.1111/febs.14643",
      "pmid": "30153368",
      "published_date": "2018 Aug 28"
    },
    "status": "released",
    "submitted_date": "2018-08-30",
    "released_date": "2018-08-31"
  },
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Beta-lactamase",
          "short_name": "OXA-48",
          "sequence": "AKEWQENKSWNAHFTEHKSQGVVVLWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALD\r\nLGVVKDEHQVFKWDGQTRDIATWNRDHNLITAMKYSVVPVYQEFARQIGEARMSKMLHAF\r\nDYGNEDISGNVDSFWLDGGIRISATEQISFLRKLYHNKLHVSERSQRIVKQAMLTEANGD\r\nYIIRAKTGYSTRIEPKIGWWVGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEK\r\nIIP",
          "organism": "Klebsiella pneumoniae",
          "uniprot_code": "Q6XEC0",
          "uniprot_range_first": 22,
          "uniprot_range_last": 265,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVVLWNENKQQGFTNNLK\nRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIATWNRDHNLITAMKYSVV\nPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIRISATEQISFLRKLYHNK\nLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWWVGWVELDDNVWFFAMNMD\nMPTSDGLGLRQAITKEVLKQEKIIP",
          "mw": 28.218,
          "total_mw": 56.436,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM HEPES 50 mM K2SO4",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Dimeric class D beta-lactamase OXA-48",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "UiT",
            "address": "Tromso, Norway",
            "full_name": "UiT - The Arctic University of Norway",
            "webpage": "https://en.uit.no/"
          }
        ],
        "contributor_name": "Bjarte Aarmo",
        "contributor_surname": "Lund",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-02-23",
    "storage_temperature": 25.0,
    "cell_temperature": 25.0,
    "exposure_time": 10.0,
    "number_of_frames": 10,
    "wavelength": 0.09919,
    "sample_detector_distance": 2.867,
    "concentration_min": 0.1,
    "concentration_max": 13.0,
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  "experiment_description": "Synchrotron SAXS data from solutions of dimeric class D beta-lactamase (OXA-48) in 50 mM HEPES, 50 mM K2SO4, pH 7, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.1 and 13 mg/ml were measured at 25°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration(s) were merged with the higher concentration high angle data to yield the final composite scattering curve.",
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  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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