Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 0.2 M D-glucosamine

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    "title": "Molecular Fingerprints for a Novel Enzyme Family in <i>Actinobacteria</i> with Glucosamine Kinase Activity.",
    "publication": {
      "title": "Molecular Fingerprints for a Novel Enzyme Family in <i>Actinobacteria</i> with Glucosamine Kinase Activity.",
      "author_list": "Manso JA, Nunes-Costa D, Macedo-Ribeiro S, Empadinhas N, Pereira PJB",
      "journal": "MBio",
      "doi": "10.1128/mBio.00239-19",
      "pmid": "31088917",
      "published_date": "2019 May 14"
    },
    "status": "released",
    "submitted_date": "2018-10-15",
    "released_date": "2019-05-09"
  },
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Glucosamine kinase",
          "short_name": null,
          "sequence": "MTPNWSELVAAADPALVLPSGERRAEVAVPGPLRLDALLDLGEGHAVGVVRSADAARWTV\r\nPLVRDGAGGVRRSRPGDGTAEHLVAALARRGATPDAAFVLEAFTGAAPVTGERGIIVDQT\r\nNESVIVGECAVVKWAVRLPAEGEPGSPAAQRIAALARGGFTEMPRPWGLLTLAEGAQPVL\r\nLASVVAYLPGALDGWDWAVDDVRRLARGELTMDQALLPAAQLGTLTARMHAALAARGRTP\r\nATAADVAAWGVRMREELDEAVASVPGAEGERLKAWAPRIADVYAELDALAGTPLIDVHGD\r\nFHVGQILRADGRYAVVDFDGNPVLPADQRAARQPAALDVVGMTASLDHVGRVVVFRTPDV\r\nDPAPVRAWIAAAQRSFLDAYRTTLARLDADDLFDDRLLTPLRYAQEVREYLYAVRHLPHW\r\nVYVPDLSLTDLLPERLKDKKLAAALEHHHHHH",
          "organism": "Streptacidiphilus jiangxiensis",
          "uniprot_code": "A0A1H7TQR5",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MTPNWSELVAAADPALVLPSGERRAEVAVPGPLRLDALLDLGEGHAVGVVRSADAARWTV\nPLVRDGAGGVRRSRPGDGTAEHLVAALARRGATPDAAFVLEAFTGAAPVTGERGIIVDQT\nNESVIVGECAVVKWAVRLPAEGEPGSPAAQRIAALARGGFTEMPRPWGLLTLAEGAQPVL\nLASVVAYLPGALDGWDWAVDDVRRLARGELTMDQALLPAAQLGTLTARMHAALAARGRTP\nATAADVAAWGVRMREELDEAVASVPGAEGERLKAWAPRIADVYAELDALAGTPLIDVHGD\nFHVGQILRADGRYAVVDFDGNPVLPADQRAARQPAALDVVGMTASLDHVGRVVVFRTPDV\nDPAPVRAWIAAAQRSFLDAYRTTLARLDADDLFDDRLLTPLRYAQEVREYLYAVRHLPHW\nVYVPDLSLTDLLPERLKD",
          "mw": 48.377,
          "total_mw": 48.377,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 0.2 M D-glucosamine",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 0.2 M D-glucosamine",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC-CSIC-Universidad de Cantabria)",
            "webpage": null
          }
        ],
        "contributor_name": "Jose A",
        "contributor_surname": "Manso",
        "orcid": "https://orcid.org/0000-0002-2565-6239"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-11-20",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 1.0,
    "number_of_frames": 10,
    "wavelength": 0.09919,
    "sample_detector_distance": 2.867,
    "concentration_min": 1.25,
    "concentration_max": 5.0,
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    "flow_rate": null,
    "s_min": 0.057,
    "s_max": 4.934,
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          "comment": "Volume fraction for this structure was of 1.0 as determined OLIGOMER",
          "user": 320
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/gluk01_02M_GlcN_zero_OLIGOMER_SASBDB_2nVzqoL_fit_img.png",
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      "chi_square_value": 7.4,
      "p_value": 0.0,
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  "pddf_software_version": "5.0",
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  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Streptacidiphilus jiangxiensis glucosamine kinase in presence of 0.2 M D-glucosamine in 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM MgCl2 and 5 mM DTT were collected on the BM29 BIOSAXS beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.867 m and at a wavelength of λ = 0.0992 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle; s-range = 0.0385 < s < 4.934 nm-1). Solute concentrations ranging between 1.25 and 5.0 mg/ml were measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution.",
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  "guinier_i0_error": 0.05,
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  "guinier_rg": 2.57,
  "guinier_rg_error": 0.02,
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  "pddf_dmax_error": null,
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  "porod_volume_error": null,
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  "guinier_point_last": 96,
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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