| code | SASDEM9 |
| status | Published |
| type_of_curve | SEC-SAS |
| angular_unit | 1/A |
| project.title | Complementary substrate specificity and distinct quaternary assembly of the <i>Escherichia coli</i> aerobic and anaerobic beta-oxidation trifunctional enzyme complexes. |
| project.publication.title | Complementary substrate specificity and distinct quaternary assembly of the <i>Escherichia coli</i> aerobic and anaerobic beta-oxidation trifunctional enzyme complexes. |
| project.publication.author_list | Sah-Teli SK, Hynönen MJ, Schmitz W, Geraets JA, Seitsonen J, Pedersen JS, Butcher SJ, Wierenga RK, Venkatesan R |
| project.publication.journal | Biochem J |
| project.publication.doi | 10.1042/BCJ20190314 |
| project.publication.pmid | 31235482 |
| project.publication.published_date | 2019 Jun 25 |
| project.status | released |
| project.submitted_date | 2018-12-28 |
| project.released_date | 2019-07-02 |
| pddf_data | https://www.sasbdb.org/media/p_of_R_files/SASDEM9.out |
| intensities_data | https://www.sasbdb.org/media/intensities_files/SASDEM9.dat |
| intensities_log_plot | https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEM9_dat_img.png |
| intensities_kratky_plot | https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEM9_kratky_img.png |
| pddf_plot | https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEM9_pofr_img.png |
| intensities_guinier_plot | https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEM9_guinier_img.png |
| sascif_data | https://www.sasbdb.org/media/sascif/sascif_files/SASDEM9.sascif |
| experiment.instrument.detector.type | Dectris |
| experiment.instrument.detector.name | Pilatus 1M |
| experiment.instrument.detector.resolution | 172.0 |
| experiment.instrument.name | ESRF |
| experiment.instrument.city | Grenoble |
| experiment.instrument.country | France |
| experiment.instrument.beamline_name | BM29 |
| experiment.instrument.beam_geometry | |
| experiment.instrument.type_of_source | X-ray synchrotron |
| experiment.instrument.point_source | null |
| experiment.instrument.line_collimation | null |
| experiment.instrument.sample_path_length | null |
| experiment.instrument.line_collimation_slitlength | null |
| experiment.instrument.line_collimation_integrationwidth | null |
| experiment.instrument.xray_energy | null |
| experiment.instrument.beam_profile_ah | null |
| experiment.instrument.beam_profile_al | null |
| experiment.sample.molecule[0].long_name | Fatty acid oxidation complex subunit alpha |
| experiment.sample.molecule[0].short_name | anEcTFE-alpha |
| experiment.sample.molecule[0].sequence | MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIG
NCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGR
ALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVK
FGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVV
AIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[0].organism | Escherichia coli (strain K12) |
| experiment.sample.molecule[0].uniprot_code | P77399 |
| experiment.sample.molecule[0].uniprot_range_first | 1 |
| experiment.sample.molecule[0].uniprot_range_last | 714 |
| experiment.sample.molecule[0].oligomerization | monomer |
| experiment.sample.molecule[0].molecular_type | protein |
| experiment.sample.molecule[0].uniprot_sequence | MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSA
KPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELA
LACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQAL
KLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKT
QGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKV
RRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIF
ASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQ
GKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVG
IDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLG
GPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[0].mw | 77.071 |
| experiment.sample.molecule[0].total_mw | 77.071 |
| experiment.sample.molecule[0].number_molecules | 1 |
| experiment.sample.molecule[0].complex_state | False |
| experiment.sample.molecule[0].deuteration | null |
| experiment.sample.molecule[0].molecule_source | biological |
| experiment.sample.molecule[0].molecule_description | null |
| experiment.sample.molecule[1].long_name | anaerobic Fatty acid oxidation complex subunit alpha |
| experiment.sample.molecule[1].short_name | anEcTFE-alpha |
| experiment.sample.molecule[1].sequence | >anEcTFEalpha
MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIG
NCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSG
GTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGR
ALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLA
LISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVK
FGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVV
AIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[1].organism | Escherichia coli |
| experiment.sample.molecule[1].uniprot_code | P77399 |
| experiment.sample.molecule[1].uniprot_range_first | null |
| experiment.sample.molecule[1].uniprot_range_last | null |
| experiment.sample.molecule[1].oligomerization | monomer |
| experiment.sample.molecule[1].molecular_type | protein |
| experiment.sample.molecule[1].uniprot_sequence | MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSA
KPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELA
LACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQAL
KLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKT
QGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKV
RRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIF
ASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQ
GKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVG
IDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLG
GPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[1].mw | 77.071 |
| experiment.sample.molecule[1].total_mw | 77.071 |
| experiment.sample.molecule[1].number_molecules | 1 |
| experiment.sample.molecule[1].complex_state | False |
| experiment.sample.molecule[1].deuteration | null |
| experiment.sample.molecule[1].molecule_source | biological |
| experiment.sample.molecule[1].molecule_description | null |
| experiment.sample.molecule[2].long_name | anaerobic Fatty acid oxidation complex subunit alpha |
| experiment.sample.molecule[2].short_name | anEcTFE-alpha |
| experiment.sample.molecule[2].sequence | >anEcTFEalpha
MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIG
NCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSG
GTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGR
ALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLA
LISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVK
FGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVV
AIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[2].organism | Escherichia coli |
| experiment.sample.molecule[2].uniprot_code | P77399 |
| experiment.sample.molecule[2].uniprot_range_first | null |
| experiment.sample.molecule[2].uniprot_range_last | null |
| experiment.sample.molecule[2].oligomerization | monomer |
| experiment.sample.molecule[2].molecular_type | protein |
| experiment.sample.molecule[2].uniprot_sequence | MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSA
KPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELA
LACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQAL
KLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKT
QGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKV
RRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIF
ASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQ
GKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVG
IDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLG
GPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[2].mw | 77.071 |
| experiment.sample.molecule[2].total_mw | 77.071 |
| experiment.sample.molecule[2].number_molecules | 1 |
| experiment.sample.molecule[2].complex_state | False |
| experiment.sample.molecule[2].deuteration | null |
| experiment.sample.molecule[2].molecule_source | biological |
| experiment.sample.molecule[2].molecule_description | null |
| experiment.sample.molecule[3].long_name | anaerobic Fatty acid oxidation complex subunit alpha |
| experiment.sample.molecule[3].short_name | anEcTFE-alpha |
| experiment.sample.molecule[3].sequence | >anEcTFEalpha
MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIG
NCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSG
GTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGR
ALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLA
LISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVK
FGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVV
AIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[3].organism | Escherichia coli |
| experiment.sample.molecule[3].uniprot_code | P77399 |
| experiment.sample.molecule[3].uniprot_range_first | null |
| experiment.sample.molecule[3].uniprot_range_last | null |
| experiment.sample.molecule[3].oligomerization | monomer |
| experiment.sample.molecule[3].molecular_type | protein |
| experiment.sample.molecule[3].uniprot_sequence | MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSA
KPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELA
LACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQAL
KLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKT
QGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG
SDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKV
RRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIF
ASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQ
GKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVG
IDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA
IYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLG
GPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
| experiment.sample.molecule[3].mw | 77.071 |
| experiment.sample.molecule[3].total_mw | 77.071 |
| experiment.sample.molecule[3].number_molecules | 1 |
| experiment.sample.molecule[3].complex_state | False |
| experiment.sample.molecule[3].deuteration | null |
| experiment.sample.molecule[3].molecule_source | biological |
| experiment.sample.molecule[3].molecule_description | null |
| experiment.sample.molecule[4].long_name | anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit |
| experiment.sample.molecule[4].short_name | anEcTFE-beta |
| experiment.sample.molecule[4].sequence | >anEcTFEbeta
MGSSHHHHHHSQDPMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPN
IAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKA
RTMSQRLKLFSRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEE
VMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGE
VDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE |
| experiment.sample.molecule[4].organism | Escherichia coli |
| experiment.sample.molecule[4].uniprot_code | P76503 |
| experiment.sample.molecule[4].uniprot_range_first | null |
| experiment.sample.molecule[4].uniprot_range_last | null |
| experiment.sample.molecule[4].oligomerization | dimer |
| experiment.sample.molecule[4].molecular_type | protein |
| experiment.sample.molecule[4].uniprot_sequence | MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
RFAREALGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
ACAAGGLGAAMVLEAE |
| experiment.sample.molecule[4].mw | 48.143 |
| experiment.sample.molecule[4].total_mw | 96.286 |
| experiment.sample.molecule[4].number_molecules | 2 |
| experiment.sample.molecule[4].complex_state | False |
| experiment.sample.molecule[4].deuteration | null |
| experiment.sample.molecule[4].molecule_source | biological |
| experiment.sample.molecule[4].molecule_description | null |
| experiment.sample.molecule[5].long_name | anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit |
| experiment.sample.molecule[5].short_name | anEcTFE-beta |
| experiment.sample.molecule[5].sequence | >anEcTFEbeta
MGSSHHHHHHSQDPMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPN
IAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKA
RTMSQRLKLFSRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEE
VMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGE
VDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE |
| experiment.sample.molecule[5].organism | Escherichia coli |
| experiment.sample.molecule[5].uniprot_code | P76503 |
| experiment.sample.molecule[5].uniprot_range_first | null |
| experiment.sample.molecule[5].uniprot_range_last | null |
| experiment.sample.molecule[5].oligomerization | dimer |
| experiment.sample.molecule[5].molecular_type | protein |
| experiment.sample.molecule[5].uniprot_sequence | MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE
RFAREALGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
ACAAGGLGAAMVLEAE |
| experiment.sample.molecule[5].mw | 48.143 |
| experiment.sample.molecule[5].total_mw | 96.286 |
| experiment.sample.molecule[5].number_molecules | 2 |
| experiment.sample.molecule[5].complex_state | False |
| experiment.sample.molecule[5].deuteration | null |
| experiment.sample.molecule[5].molecule_source | biological |
| experiment.sample.molecule[5].molecule_description | null |
| experiment.sample.buffer.name | 50 mM Tris, 500 mM NaCl, 5% glycerol, 0.05% C12E9 (1-O-(n-Dodecyl)-nonaethyleneglycol), 2.5 mM DTT |
| experiment.sample.buffer.concentration_unit | null |
| experiment.sample.buffer.comment | null |
| experiment.sample.buffer.additive | null |
| experiment.sample.buffer.concentration | null |
| experiment.sample.buffer.pka | null |
| experiment.sample.buffer.ph | 8.0 |
| experiment.sample.buffer.deuteration | null |
| experiment.sample.purity_method | null |
| experiment.sample.name | anEcTFE: Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme complex |
| experiment.sample.ext_coefficient | null |
| experiment.sample.contrast | null |
| experiment.sample.specific_vol | null |
| experiment.sample.dry_vol | null |
| experiment.sample.absorbption | null |
| experiment.sample.deuteration | null |
| experiment.sample.mixture | null |
| experiment.contributor[0].affiliation[0].short_name | null |
| experiment.contributor[0].affiliation[0].address | Finland |
| experiment.contributor[0].affiliation[0].full_name | University of Oulu |
| experiment.contributor[0].affiliation[0].webpage | https://www.oulu.fi |
| experiment.contributor[0].contributor_name | shiv k. |
| experiment.contributor[0].contributor_surname | sah-teli |
| experiment.contributor[0].orcid | |
| experiment.concentration_method | null |
| experiment.concentration_unit | null |
| experiment.date | 2015-09-22 |
| experiment.storage_temperature | 4.0 |
| experiment.cell_temperature | 20.0 |
| experiment.exposure_time | 1.0 |
| experiment.number_of_frames | 1200 |
| experiment.wavelength | 0.1 |
| experiment.sample_detector_distance | 2.867 |
| experiment.concentration_min | null |
| experiment.concentration_max | 3.5 |
| experiment.sample_volume | null |
| experiment.flow_rate | null |
| experiment.s_min | 0.05 |
| experiment.s_max | 1.711 |
| experiment.total_exposure_time | null |
| experiment.seccolumn | null |
| fits[0].models[0].model_plot | https://www.sasbdb.org/media/pdb_file/images/SASDEM9_fit1_model1_img.png |
| fits[0].models[0].software | DAMMIN |
| fits[0].models[0].pdb_link | [] |
| fits[0].models[0].model_title | null |
| fits[0].models[0].type_of_model | dummy |
| fits[0].models[0].software_version | null |
| fits[0].models[0].model_data | https://www.sasbdb.org/media/pdb_file/SASDEM9_fit1_model1.pdb |
| fits[0].models[0].model_mw | 487.865 |
| fits[0].models[0].bead_radius | 5.0 |
| fits[0].models[0].log | https://www.sasbdb.org/media/log_files/anectfed.log |
| fits[0].models[0].symmetry | p1 |
| fits[0].models[0].comment | null |
| fits[0].models[0].user | 400 |
| fits[0].fit_unit | 1/A |
| fits[0].fit_plot | https://www.sasbdb.org/media/fitting_files/scattering_plots/anectfed_fit_img.png |
| fits[0].software | DAMMIN |
| fits[0].chi_square_value | 1.01 |
| fits[0].p_value | 0.000235 |
| fits[0].fit_residual_plot | anectfed_fitresiduals_img.png |
| fits[0].fit_data | https://www.sasbdb.org/media/fitting_files/SASDEM9_fit1.fir |
| fits[0].fit_log | https://www.sasbdb.org/media/fitting_files/log_files/anectfed.log |
| fits[0].software_version | null |
| fits[0].description | |
| fits[1].models[0].model_plot | https://www.sasbdb.org/media/pdb_file/images/SASDEM9_fit2_model1_img.png |
| fits[1].models[0].software | SwissModel |
| fits[1].models[0].pdb_link | [] |
| fits[1].models[0].model_title | null |
| fits[1].models[0].type_of_model | atomic |
| fits[1].models[0].software_version | null |
| fits[1].models[0].model_data | https://www.sasbdb.org/media/pdb_file/SASDEM9_fit2_model1.pdb |
| fits[1].models[0].model_mw | 483.1 |
| fits[1].models[0].bead_radius | null |
| fits[1].models[0].log | null |
| fits[1].models[0].symmetry | null |
| fits[1].models[0].comment | subunits and complex were modeled using swissmodel and coot respectively using 5zqz.pdb as referenc |
| fits[1].models[0].user | 400 |
| fits[1].fit_unit | 1/A |
| fits[1].fit_plot | https://www.sasbdb.org/media/fitting_files/scattering_plots/anEcTFE-modelon5zqz-octamer8-5zqz-templates00_fit_img.png |
| fits[1].software | CRYSOL |
| fits[1].chi_square_value | 3.501 |
| fits[1].p_value | 0.0 |
| fits[1].fit_residual_plot | anEcTFE-modelon5zqz-octamer8-5zqz-templates00_fitresiduals_img.png |
| fits[1].fit_data | https://www.sasbdb.org/media/fitting_files/SASDEM9_fit2.fit |
| fits[1].fit_log | https://www.sasbdb.org/media/fitting_files/log_files/anEcTFE-modelon5zqz-octamer8-5zqz-templates00.log |
| fits[1].software_version | null |
| fits[1].description | |
| estimated_volume_method | null |
| pddf_software | ATSAS GNOM |
| pddf_software_version | 5.0 |
| i0_calibration_standard | null |
| description | null |
| experiment_description | Synchrotron SAXS data from solutions of anEcTFE in 50 mM Tris, 500 mM NaCl, 5% glycerol, 0.05% C12E9 (1-O-(n-Dodecyl)-nonaethyleneglycol), 2.5 mM DTT, pH 8 were measured at 20°C using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected as 1200 successive 1 second frames through the SEC elution profile (sample concentration = 3.5 mg/ml ). The SAXS data were measured from the sample and buffer from a Superdex 200 5/150 GL, (GE Healthcare) column at flow rate of 0.16 mL/min. The sample-peak data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry. |
| tags | [] |
| intensity_unit | arbitrary |
| experimental_mw | 535.0 |
| experimental_mw_error | null |
| guinier_i0_mw | null |
| guinier_i0_mw_error | null |
| porod_mw | 535.0 |
| porod_mw_error | null |
| pddf_i0 | 58.74 |
| pddf_i0_error | null |
| guinier_i0 | 58.9 |
| guinier_i0_error | 0.14 |
| pddf_rg | 6.15 |
| pddf_rg_error | null |
| guinier_rg | 6.19 |
| guinier_rg_error | 0.02 |
| pddf_dmax | 19.57 |
| pddf_dmax_error | null |
| porod_volume | 856.0 |
| porod_volume_error | null |
| estimated_volume | null |
| estimated_volume_error | null |
| guinier_point_first | 1 |
| guinier_point_last | 35 |
| pddf_point_first | null |
| pddf_point_last | null |
| i0_calibration_standard_data | null |
| intensities_log_log_plot | SASDEM9_datloglog_img.png |
| symmetry | null |
| last_modified | 2021-02-21T15:44:53.898719+01:00 |
| bragg_peak | [] |