Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) without denaturant

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  "code": "SASDEN2",
  "status": "Published",
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  "angular_unit": "1/nm",
  "project": {
    "title": "Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.",
    "publication": {
      "title": "Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.",
      "author_list": "Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA",
      "journal": "Proc Natl Acad Sci U S A",
      "doi": "10.1073/pnas.1704692114",
      "pmid": "28716919",
      "published_date": "2017 Aug 1"
    },
    "status": "released",
    "submitted_date": "2018-08-16",
    "released_date": "2018-08-18"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDEN2.dat",
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEN2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEN2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEN2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEN2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Inner nuclear membrane protein HEH2",
          "short_name": "NLS-488/594",
          "sequence": "> U=p-acetylphenylalanine. C was labeled with Alexa Fluor 594 C5 maleimide. U was labeled with Alexa Fluor 488 C5 hydroxylamine\r\nACETNKRKREQISTDNEAKMQIQEEKSPKKKRKKRSSKANKPPEUA",
          "organism": "Saccharomyces cerevisiae",
          "uniprot_code": "Q03281",
          "uniprot_range_first": 99,
          "uniprot_range_last": 140,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MDHRNLDPKTLKVSQLRRVLVENDVAFPANARKPVLVKLFEEKVRQRLQSSPEASKVRTS\nIQKVVKSGAKNADRKKTLKSKKLESSSSESKTVKDENVETNKRKREQISTDNEAKMQIQE\nEKSPKKKRKKRSSKANKPPESPPQSKSDGKATSADLTSELETVEELHKKDSSDDKPRVKE\nLPKPELPNLKVSNEFLAQLNKELASAATENYDHSIKSTDLSSIRIETEEPVGPSTGAETR\nNESEVMENINLEVQPEVKEAKEELTKISETFDNQDEEDTSRLSSKKNIRSPKGRTRHFIA\nNKTKRGIDIMKPFIAHLFIWLWNGAIFLSIICPILFGLWYREQRIQVGYCGHEKPLKSLA\nISAFPQTERVDSVLQAYRPNCLECPEHGICSSFMNVECEPGYEPKSSILETYGIIPFPKY\nCAKDESKEKEVDELVWKVNEYLKKKNAQHECGEGENLFESGETETKLYDIFSHSRPSWES\nQREFNDHWKNVLEILKKKDDIIWLPLDFETNGKREKSKSNNTNYIYRSTSKKWVTLQCHL\nEGDIQEYITKYGGSLFITLGVLFLIKKIQSTLDNYVQGEQIIEKLVKEAIDKLKDVKKNK\nGEEPFLTTVQLRATLLSDIPNIKEQNNLWAQTKEKIMKEQSENIELYLLEENGEIMTCWE\nWKE",
          "mw": 5.406,
          "total_mw": 5.406,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Alexa Fluor™ 594 C5 Maleimide",
          "short_name": "Alexa594",
          "sequence": null,
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 0.886,
          "total_mw": 0.886,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Alexa Fluor™ 488 C5 Hydroxylamine",
          "short_name": "Alexa488",
          "sequence": null,
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 0.7,
          "total_mw": 0.7,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM HEPES, 150 mM NaCl, 10 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) without denaturant",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Prague, Czech Republic",
            "full_name": "Institute of Biotechnology CAS v.v.i.",
            "webpage": "http://www.ibt.cas.cz/"
          }
        ],
        "contributor_name": "Gustavo",
        "contributor_surname": "Fuertes Vives",
        "orcid": "https://orcid.org/0000-0002-8564-8644"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2014-01-25",
    "storage_temperature": null,
    "cell_temperature": 23.0,
    "exposure_time": 0.05,
    "number_of_frames": 20,
    "wavelength": 0.1,
    "sample_detector_distance": 3.0,
    "concentration_min": 1.5,
    "concentration_max": 7.5,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.02,
    "s_max": 4.509,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).",
  "experiment_description": null,
  "tags": [
    "idp"
  ],
  "intensity_unit": "arbitrary",
  "experimental_mw": 8.5,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 224.4,
  "pddf_i0_error": 3.5,
  "guinier_i0": 223.9,
  "guinier_i0_error": 2.9,
  "pddf_rg": 2.166,
  "pddf_rg_error": 0.03,
  "guinier_rg": 2.0,
  "guinier_rg_error": 0.2,
  "pddf_dmax": 7.29,
  "pddf_dmax_error": null,
  "porod_volume": 9.9,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 27,
  "guinier_point_last": 236,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDEN2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
  "bragg_peak": []
}

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