SASDEQ2

Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) with denaturant

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00

The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).

▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) with denaturant

Concentration— – 2.5 Buffer / pH25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl / 7.4
Experimental temperature23.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.1 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Inner nuclear membrane protein HEH2
View sequence
>U=P-ACETYLPHENYLALANINE.CWASLABELEDWITHALEXAFLUOR594C5MALEIMIDE.UWASLABELEDWITHALEXAFLUOR488C5HYDROXYLAMINEACETNKRKREQISTDNEAKMQIQEEKSPKKKRKKRSSKANKPPEUA
proteinSaccharomyces cerevisiaeQ0328199–140monomerMolecule count 15.406 kDa
Alexa Fluor™ 594 C5 Maleimideother———–—monomerMolecule count 10.886 kDa
Alexa Fluor™ 488 C5 Hydroxylamineother———–—monomerMolecule count 10.7 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11705[3]0.0199359–4.50939 1/nmHas uncertainty columnMissing 0125sasbdb/entries/q2/sasdeq2/source/SASDEQ2.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)8.1—nm
i0Guinier208.22.6arbitrary
i0P(r)208.64.0arbitrary
mwExperimental7.3—kDa
porod_volumePorod12.19—nm³
rgGuinier2.40.2nm
rgP(r)2.5940.07nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

▶

4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2014-01-25Storage / measurement temperature— / 23.0
Exposure time0.05Frames20
Wavelength0.1Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.02 – 4.509Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
curvesasbdb/entries/q2/sasdeq2/source/SASDEQ2.datdownloaded77111ac012477eafa900fda332f0885bbb3ab666e7f41ada5227bf26a09bb99e7e4e7DownloadView source fileSource site
full_entry_zipsasbdb/entries/q2/sasdeq2/source/SASDEQ2.zipdownloaded43750926d63f63634bc539cc9e37efb1bce6f66e317a4b9093b5bc84a8c20f698a7ecDownloadView source fileSource site
pddfsasbdb/entries/q2/sasdeq2/source/SASDEQ2.outdownloaded87705b93b0a2d7fecb7148223ce4b01a1168511a2eac1dbd2d7cac4df6466b1fb032dDownloadView source fileSource site
sascifsasbdb/entries/q2/sasdeq2/source/SASDEQ2.sascifdownloaded1535170de7088e0449d4fb5d1c7b554f524a2014e778951f430e45ef989e4c7249cfcfDownloadView source fileSource site
summarysasbdb/entries/q2/sasdeq2/source/summary.jsondownloaded796168aaac1bb9908c449e1b25c49195976a4442ebb144d165b2860e2e6ac1605620DownloadView source fileSource site
curve:source record
{
  "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.dat",
  "role": "curve",
  "sha256": "ac012477eafa900fda332f0885bbb3ab666e7f41ada5227bf26a09bb99e7e4e7",
  "size": 77111,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDEQ2.dat"
}
full_entry_zip:source record and ZIP contents (2 items)
{
  "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.zip",
  "role": "full_entry_zip",
  "sha256": "926d63f63634bc539cc9e37efb1bce6f66e317a4b9093b5bc84a8c20f698a7ec",
  "size": 43750,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDEQ2.zip",
  "zip_member_count": 2,
  "zip_members": [
    {
      "crc32": "dbda07fc",
      "name": "SASDEQ2/experimental_data/SASDEQ2.dat",
      "size": 77111
    },
    {
      "crc32": "c6289c26",
      "name": "SASDEQ2/pddf/SASDEQ2.out",
      "size": 87705
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:source record
{
  "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.out",
  "role": "pddf",
  "sha256": "b93b0a2d7fecb7148223ce4b01a1168511a2eac1dbd2d7cac4df6466b1fb032d",
  "size": 87705,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDEQ2.out"
}
sascif:source record
{
  "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.sascif",
  "role": "sascif",
  "sha256": "0de7088e0449d4fb5d1c7b554f524a2014e778951f430e45ef989e4c7249cfcf",
  "size": 153517,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEQ2.sascif"
}
summary:source record
{
  "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/summary.json",
  "sha256": "68aaac1bb9908c449e1b25c49195976a4442ebb144d165b2860e2e6ac1605620",
  "size": 7961,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDEQ2/"
}

All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:168 field values
Field pathRaw value
codeSASDEQ2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleDecoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
project.publication.titleDecoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
project.publication.author_listFuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
project.publication.journalProc Natl Acad Sci U S A
project.publication.doi10.1073/pnas.1704692114
project.publication.pmid28716919
project.publication.published_date2017 Aug 1
project.statusreleased
project.submitted_date2018-08-16
project.released_date2018-08-18
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDEQ2.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDEQ2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEQ2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEQ2_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEQ2_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEQ2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDEQ2.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameInner nuclear membrane protein HEH2
experiment.sample.molecule[0].short_nameNLS-488/594
experiment.sample.molecule[0].sequence> U=p-acetylphenylalanine. C was labeled with Alexa Fluor 594 C5 maleimide. U was labeled with Alexa Fluor 488 C5 hydroxylamine ACETNKRKREQISTDNEAKMQIQEEKSPKKKRKKRSSKANKPPEUA
experiment.sample.molecule[0].organismSaccharomyces cerevisiae
experiment.sample.molecule[0].uniprot_codeQ03281
experiment.sample.molecule[0].uniprot_range_first99
experiment.sample.molecule[0].uniprot_range_last140
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMDHRNLDPKTLKVSQLRRVLVENDVAFPANARKPVLVKLFEEKVRQRLQSSPEASKVRTS IQKVVKSGAKNADRKKTLKSKKLESSSSESKTVKDENVETNKRKREQISTDNEAKMQIQE EKSPKKKRKKRSSKANKPPESPPQSKSDGKATSADLTSELETVEELHKKDSSDDKPRVKE LPKPELPNLKVSNEFLAQLNKELASAATENYDHSIKSTDLSSIRIETEEPVGPSTGAETR NESEVMENINLEVQPEVKEAKEELTKISETFDNQDEEDTSRLSSKKNIRSPKGRTRHFIA NKTKRGIDIMKPFIAHLFIWLWNGAIFLSIICPILFGLWYREQRIQVGYCGHEKPLKSLA ISAFPQTERVDSVLQAYRPNCLECPEHGICSSFMNVECEPGYEPKSSILETYGIIPFPKY CAKDESKEKEVDELVWKVNEYLKKKNAQHECGEGENLFESGETETKLYDIFSHSRPSWES QREFNDHWKNVLEILKKKDDIIWLPLDFETNGKREKSKSNNTNYIYRSTSKKWVTLQCHL EGDIQEYITKYGGSLFITLGVLFLIKKIQSTLDNYVQGEQIIEKLVKEAIDKLKDVKKNK GEEPFLTTVQLRATLLSDIPNIKEQNNLWAQTKEKIMKEQSENIELYLLEENGEIMTCWE WKE
experiment.sample.molecule[0].mw5.406
experiment.sample.molecule[0].total_mw5.406
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameAlexa Fluor™ 594 C5 Maleimide
experiment.sample.molecule[1].short_nameAlexa594
experiment.sample.molecule[1].sequencenull
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeother
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw0.886
experiment.sample.molecule[1].total_mw0.886
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_statenull
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.molecule[2].long_nameAlexa Fluor™ 488 C5 Hydroxylamine
experiment.sample.molecule[2].short_nameAlexa488
experiment.sample.molecule[2].sequencenull
experiment.sample.molecule[2].organismnull
experiment.sample.molecule[2].uniprot_codenull
experiment.sample.molecule[2].uniprot_range_firstnull
experiment.sample.molecule[2].uniprot_range_lastnull
experiment.sample.molecule[2].oligomerizationmonomer
experiment.sample.molecule[2].molecular_typeother
experiment.sample.molecule[2].uniprot_sequencenull
experiment.sample.molecule[2].mw0.7
experiment.sample.molecule[2].total_mw0.7
experiment.sample.molecule[2].number_molecules1
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_descriptionnull
experiment.sample.buffer.name25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLabeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) with denaturant
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressPrague, Czech Republic
experiment.contributor[0].affiliation[0].full_nameInstitute of Biotechnology CAS v.v.i.
experiment.contributor[0].affiliation[0].webpagehttp://www.ibt.cas.cz/
experiment.contributor[0].contributor_nameGustavo
experiment.contributor[0].contributor_surnameFuertes Vives
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-8564-8644
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-01-25
experiment.storage_temperaturenull
experiment.cell_temperature23.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.1
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max2.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.02
experiment.s_max4.509
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).
experiment_descriptionnull
tags[0]idp
intensity_unitarbitrary
experimental_mw7.3
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0208.6
pddf_i0_error4.0
guinier_i0208.2
guinier_i0_error2.6
pddf_rg2.594
pddf_rg_error0.07
guinier_rg2.4
guinier_rg_error0.2
pddf_dmax8.1
pddf_dmax_errornull
porod_volume12.19
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first30
guinier_point_last196
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEQ2_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDEQ2
statussuccess
started_at2026-08-11T13:43:40.037328+00:00
finished_at2026-08-11T13:43:48.891648+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDEQ2/
files[0].path/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/summary.json
files[0].size7961
files[0].sha25668aaac1bb9908c449e1b25c49195976a4442ebb144d165b2860e2e6ac1605620
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDEQ2.dat
files[1].path/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.dat
files[1].size77111
files[1].sha256ac012477eafa900fda332f0885bbb3ab666e7f41ada5227bf26a09bb99e7e4e7
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDEQ2.out
files[2].path/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.out
files[2].size87705
files[2].sha256b93b0a2d7fecb7148223ce4b01a1168511a2eac1dbd2d7cac4df6466b1fb032d
files[2].rolepddf
files[3].statusdownloaded
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDEQ2.sascif
files[3].path/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.sascif
files[3].size153517
files[3].sha2560de7088e0449d4fb5d1c7b554f524a2014e778951f430e45ef989e4c7249cfcf
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDEQ2.zip
files[4].path/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.zip
files[4].size43750
files[4].sha256926d63f63634bc539cc9e37efb1bce6f66e317a4b9093b5bc84a8c20f698a7ec
files[4].rolefull_entry_zip
files[4].zip_member_count2
files[4].zip_validationcentral_directory_readable
View the complete summary.json source
{
  "code": "SASDEQ2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.",
    "publication": {
      "title": "Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.",
      "author_list": "Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA",
      "journal": "Proc Natl Acad Sci U S A",
      "doi": "10.1073/pnas.1704692114",
      "pmid": "28716919",
      "published_date": "2017 Aug 1"
    },
    "status": "released",
    "submitted_date": "2018-08-16",
    "released_date": "2018-08-18"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDEQ2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDEQ2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEQ2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEQ2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEQ2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEQ2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEQ2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Inner nuclear membrane protein HEH2",
          "short_name": "NLS-488/594",
          "sequence": "> U=p-acetylphenylalanine. C was labeled with Alexa Fluor 594 C5 maleimide. U was labeled with Alexa Fluor 488 C5 hydroxylamine\r\nACETNKRKREQISTDNEAKMQIQEEKSPKKKRKKRSSKANKPPEUA",
          "organism": "Saccharomyces cerevisiae",
          "uniprot_code": "Q03281",
          "uniprot_range_first": 99,
          "uniprot_range_last": 140,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MDHRNLDPKTLKVSQLRRVLVENDVAFPANARKPVLVKLFEEKVRQRLQSSPEASKVRTS\nIQKVVKSGAKNADRKKTLKSKKLESSSSESKTVKDENVETNKRKREQISTDNEAKMQIQE\nEKSPKKKRKKRSSKANKPPESPPQSKSDGKATSADLTSELETVEELHKKDSSDDKPRVKE\nLPKPELPNLKVSNEFLAQLNKELASAATENYDHSIKSTDLSSIRIETEEPVGPSTGAETR\nNESEVMENINLEVQPEVKEAKEELTKISETFDNQDEEDTSRLSSKKNIRSPKGRTRHFIA\nNKTKRGIDIMKPFIAHLFIWLWNGAIFLSIICPILFGLWYREQRIQVGYCGHEKPLKSLA\nISAFPQTERVDSVLQAYRPNCLECPEHGICSSFMNVECEPGYEPKSSILETYGIIPFPKY\nCAKDESKEKEVDELVWKVNEYLKKKNAQHECGEGENLFESGETETKLYDIFSHSRPSWES\nQREFNDHWKNVLEILKKKDDIIWLPLDFETNGKREKSKSNNTNYIYRSTSKKWVTLQCHL\nEGDIQEYITKYGGSLFITLGVLFLIKKIQSTLDNYVQGEQIIEKLVKEAIDKLKDVKKNK\nGEEPFLTTVQLRATLLSDIPNIKEQNNLWAQTKEKIMKEQSENIELYLLEENGEIMTCWE\nWKE",
          "mw": 5.406,
          "total_mw": 5.406,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Alexa Fluor™ 594 C5 Maleimide",
          "short_name": "Alexa594",
          "sequence": null,
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 0.886,
          "total_mw": 0.886,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Alexa Fluor™ 488 C5 Hydroxylamine",
          "short_name": "Alexa488",
          "sequence": null,
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 0.7,
          "total_mw": 0.7,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) with denaturant",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Prague, Czech Republic",
            "full_name": "Institute of Biotechnology CAS v.v.i.",
            "webpage": "http://www.ibt.cas.cz/"
          }
        ],
        "contributor_name": "Gustavo",
        "contributor_surname": "Fuertes Vives",
        "orcid": "https://orcid.org/0000-0002-8564-8644"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2014-01-25",
    "storage_temperature": null,
    "cell_temperature": 23.0,
    "exposure_time": 0.05,
    "number_of_frames": 20,
    "wavelength": 0.1,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
    "concentration_max": 2.5,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.02,
    "s_max": 4.509,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).",
  "experiment_description": null,
  "tags": [
    "idp"
  ],
  "intensity_unit": "arbitrary",
  "experimental_mw": 7.3,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 208.6,
  "pddf_i0_error": 4.0,
  "guinier_i0": 208.2,
  "guinier_i0_error": 2.6,
  "pddf_rg": 2.594,
  "pddf_rg_error": 0.07,
  "guinier_rg": 2.4,
  "guinier_rg_error": 0.2,
  "pddf_dmax": 8.1,
  "pddf_dmax_error": null,
  "porod_volume": 12.19,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 30,
  "guinier_point_last": 196,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDEQ2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
  "bragg_peak": []
}
View the complete manifest.json source
{
  "code": "SASDEQ2",
  "status": "success",
  "started_at": "2026-08-11T13:43:40.037328+00:00",
  "finished_at": "2026-08-11T13:43:48.891648+00:00",
  "source_last_modified": "2023-06-21T07:56:39.492710+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDEQ2/",
      "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/summary.json",
      "size": 7961,
      "sha256": "68aaac1bb9908c449e1b25c49195976a4442ebb144d165b2860e2e6ac1605620"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDEQ2.dat",
      "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.dat",
      "size": 77111,
      "sha256": "ac012477eafa900fda332f0885bbb3ab666e7f41ada5227bf26a09bb99e7e4e7",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDEQ2.out",
      "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.out",
      "size": 87705,
      "sha256": "b93b0a2d7fecb7148223ce4b01a1168511a2eac1dbd2d7cac4df6466b1fb032d",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEQ2.sascif",
      "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.sascif",
      "size": 153517,
      "sha256": "0de7088e0449d4fb5d1c7b554f524a2014e778951f430e45ef989e4c7249cfcf",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDEQ2.zip",
      "path": "/data/saxsdb/sasbdb/entries/q2/sasdeq2/source/SASDEQ2.zip",
      "size": 43750,
      "sha256": "926d63f63634bc539cc9e37efb1bce6f66e317a4b9093b5bc84a8c20f698a7ec",
      "role": "full_entry_zip",
      "zip_member_count": 2,
      "zip_validation": "central_directory_readable"
    }
  ]
}