The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
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{
"code": "SASDER8",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/nm",
"project": {
"title": "Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4.",
"publication": {
"title": "Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4.",
"author_list": "Giménez-Mascarell P, Oyenarte I, González-Recio I, Fernández-Rodríguez C, Corral-Rodríguez MÁ, Campos-Zarraga I, Simón J, Kostantin E, Hardy S, Díaz Quintana A, Zubillaga Lizeaga M, Merino N, Diercks T, Blanco FJ, Díaz Moreno I, Martínez-Chantar ML, Tremblay ML, Müller D, Siliqi D, Martínez-Cruz LA",
"journal": "Int J Mol Sci",
"doi": "10.3390/ijms20246279",
"pmid": "31842432",
"published_date": "2019 Dec 12"
},
"status": "released",
"submitted_date": "2018-12-06",
"released_date": "2020-01-09"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDER8.dat",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDER8.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDER8_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDER8_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDER8_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDER8_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDER8.sascif",
"experiment": {
"instrument": {
"detector": {
"type": null,
"name": "Pilatus 2M",
"resolution": null
},
"name": "Diamond Light Source",
"city": "Didcot",
"country": "UK",
"beamline_name": "B21",
"beam_geometry": "1 x 5 mm",
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "CNNM4_cNMP",
"short_name": null,
"sequence": "KISPQLLLAAHRFLATEVSQFSPSLISEKILLRLLKYPDVIQELKFDEHNKYYARH\r\nYLYTRNKPADYFILILQGKVEVEAGKENMKFETGAFSYYGTMALTSVPSDRSPAHPTPLS\r\nRSASLSYPDRTDVSTAATLAGSSNQFGSSVLGQYISDFSVRALVDLQYIKITRQQYQNGL\r\nLASRMENSPQ",
"organism": "Homo sapiens",
"uniprot_code": null,
"uniprot_range_first": null,
"uniprot_range_last": null,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": null,
"mw": 20.937,
"total_mw": 41.874,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.4,
"deuteration": null
},
"purity_method": null,
"name": "Cyclic nucleotide homology domain of the human Cyclin and CBS domain divalent metal cation transport mediator-4",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Bari, Italy",
"full_name": "CNR - Institute of Crystallography, Bari, Italy",
"webpage": "http://www.ba.ic.cnr.it"
}
],
"contributor_name": "Dritan",
"contributor_surname": "Siliqi",
"orcid": "https://orcid.org/0000-0003-1415-8725"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2018-02-07",
"storage_temperature": null,
"cell_temperature": 20.0,
"exposure_time": 3.0,
"number_of_frames": 620,
"wavelength": 0.1,
"sample_detector_distance": 4.014,
"concentration_min": null,
"concentration_max": 7.0,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.083,
"s_max": 2.24,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDER8_fit1_model1_img.png",
"software": "GASBOR",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": "2.3i",
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDER8_fit1_model1.pdb",
"model_mw": null,
"bead_radius": 1.9,
"log": "https://www.sasbdb.org/media/log_files/GASBORon_247.log",
"symmetry": null,
"comment": null,
"user": 396
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDER8_fit1_fixed_fit_img.png",
"software": "GASBOR",
"chi_square_value": 2.79,
"p_value": 0.185286,
"fit_residual_plot": "SASDER8_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDER8_fit1.fir",
"fit_log": null,
"software_version": "2.3i",
"description": ""
},
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDER8_fit2_model1_img.png",
"software": "PISA",
"pdb_link": [
{
"pdb_code": "6g52",
"ext_code": "6G52",
"ext_type": "pdb",
"difference_with_model": "identical"
}
],
"model_title": null,
"type_of_model": "atomic",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDER8_fit2_model1.pdb",
"model_mw": 29.84,
"bead_radius": null,
"log": null,
"symmetry": null,
"comment": null,
"user": 396
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDER8_fit2_fixed_fit_img.png",
"software": "CRYSOL",
"chi_square_value": 2.93,
"p_value": 0.131689,
"fit_residual_plot": "SASDER8_fit2_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDER8_fit2.fit",
"fit_log": null,
"software_version": null,
"description": ""
}
],
"estimated_volume_method": null,
"pddf_software": "ScÅtter",
"pddf_software_version": "3.1v",
"i0_calibration_standard": null,
"description": "SEC-SAXS was performed at 20°C using the following parameters: Column: Shodex KW-403 ; Flow rate: 0.08 mL/min; Total acquisition time: 1860s; Sample injection concentration: 7 mg/mL; Injection volume: 45μL.",
"experiment_description": "Synchrotron SAXS data from solutions of Cyclic nucleotide homology domain of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH 7.4 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 camera at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample elution peak (collected as 620 successive 3 second frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.",
"tags": [],
"intensity_unit": null,
"experimental_mw": 32.0,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 54.0,
"porod_mw_error": null,
"pddf_i0": 0.002439,
"pddf_i0_error": 2.979e-05,
"guinier_i0": 0.002476,
"guinier_i0_error": 2.975e-05,
"pddf_rg": 2.57,
"pddf_rg_error": 0.13,
"guinier_rg": 2.552,
"guinier_rg_error": 0.165,
"pddf_dmax": 9.65,
"pddf_dmax_error": null,
"porod_volume": 102.0,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 22,
"guinier_point_last": 188,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDER8_datloglog_img.png",
"symmetry": null,
"last_modified": "2020-01-09T14:41:53.250881+01:00",
"bragg_peak": []
}
