查看完整 summary.json 原文
{
"code": "SASDES5",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/nm",
"project": {
"title": "Structural characterization of the third scavenger receptor cysteine-rich domain of murine neurotrypsin.",
"publication": {
"title": "Structural characterization of the third scavenger receptor cysteine-rich domain of murine neurotrypsin.",
"author_list": "Canciani A, Catucci G, Forneris F",
"journal": "Protein Sci",
"doi": "10.1002/pro.3587",
"pmid": "30748049",
"published_date": "2019 Apr"
},
"status": "released",
"submitted_date": "2018-10-03",
"released_date": "2019-02-13"
},
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"experiment": {
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},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
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"type_of_source": "X-ray synchrotron",
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},
"sample": {
"molecule": [
{
"long_name": "Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3",
"short_name": "mmNT-SRCR3",
"sequence": "GTGEFTSGSGFPIRLVDGENKKEGRVEVFVNGQWGTICDDGWTDKHAAVICRQLGYKGPARARTMAYFGEGKGPIHMDNVKCTGNEKALADCVKQDIGRHNCRHSEDAGVICDYLEKKASS",
"organism": "Mus musculus",
"uniprot_code": "O08762",
"uniprot_range_first": 383,
"uniprot_range_last": 494,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MALARCVLAVILGALSVVARADPVSRSPLHRPHPSPPRSQHAHYLPSSRRPPRTPRFPLP\nLRIPAAQRPQVLSTGHTPPTIPRRCGAGESWGNATNLGVPCLHWDEVPPFLERSPPASWA\nELRGQPHNFCRSPDGSGRPWCFYRNAQGKVDWGYCDCGQGPALPVIRLVGGNSGHEGRVE\nLYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQAHFGEGSGPILLDEVRCTGNEL\nSIEQCPKSSWGEHNCGHKEDAGVSCVPLTDGVIRLAGGKSTHEGRLEVYYKGQWGTVCDD\nGWTEMNTYVACRLLGFKYGKQSSVNHFDGSNRPIWLDDVSCSGKEVSFIQCSRRQWGRHD\nCSHREDVGLTCYPDSDGHRLSPGFPIRLVDGENKKEGRVEVFVNGQWGTICDDGWTDKHA\nAVICRQLGYKGPARARTMAYFGEGKGPIHMDNVKCTGNEKALADCVKQDIGRHNCRHSED\nAGVICDYLEKKASSSGNKEMLSSGCGLRLLHRRQKRIIGGNNSLRGAWPWQASLRLRSAH\nGDGRLLCGATLLSSCWVLTAAHCFKRYGNNSRSYAVRVGDYHTLVPEEFEQEIGVQQIVI\nHRNYRPDRSDYDIALVRLQGPGEQCARLSTHVLPACLPLWRERPQKTASNCHITGWGDTG\nRAYSRTLQQAAVPLLPKRFCKERYKGLFTGRMLCAGNLQEDNRVDSCQGDSGGPLMCEKP\nDESWVVYGVTSWGYGCGVKDTPGVYTRVPAFVPWIKSVTSL",
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"number_molecules": 1,
"complex_state": false,
"deuteration": null,
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"molecule_description": null
}
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"pka": null,
"ph": 8.0,
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},
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"name": "Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 (mmNT-SRCR3) - from SEC-SAXS",
"ext_coefficient": null,
"contrast": null,
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},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Italy",
"full_name": "University of Pavia",
"webpage": "http://wcm-3.unipv.it"
}
],
"contributor_name": "Federico",
"contributor_surname": "Forneris",
"orcid": null
}
],
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"date": "2017-05-20",
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"cell_temperature": 20.0,
"exposure_time": 1.0,
"number_of_frames": 22,
"wavelength": 0.09919,
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},
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"user": 294
}
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"experiment_description": "Synchrotron SAXS data, I(s) vs s (where s = 4πsinθ/λ, 2θ is the scattering angle and λ the X-ray wavelength), were collected from a sample of mmNT-SRCR3 using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line of the ESRF (Grenoble France) coupled to a Nexera High Pressure Liquid/Chromatography (HPLC; Shimadzu) system. Data were collected using a Pilatus 1M detector at a sample-detector distance of 2.87 m (s-range; 0.03–4.5 nm-1) at a wavelength of λ = 0.09919 nm (12.5 keV). The SEC mobile phase consisted of 25 mM HEPES/NaOH, 0.1 M NaCl, pH 8, (20°C). The protein was concentrated to 15 mg/mL and a 15 µL of sample was run on a Superdex 75 Increase 3.2/300 column (GE Healthcare). Analysis and of the scattering intensities measured from the SEC-elution sample peak (22 successive 1 s frames) and the subtraction of intensities measured from an appropriate solvent-blank were was performed using CHROMIXS of the ATSAS suite. Comparison of in-solution scattering to crystallographic data was carried out with CRYSOL.",
"tags": [],
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"symmetry": null,
"last_modified": "2024-06-23T13:31:55.412859+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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